Category Archives: Techniques

May 5, 2022

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Today’s digest features a review on the role of bacterial translocation (especially oral-gut axis) in health and disease, a proof-of-concept study on probiotics on processing of negative emotions in humans, and more. Happy reading!

Let’s talk microbiome science at the next #MVIF!

Sterile (?) environment

Comprehensive human amniotic fluid metagenomics supports the sterile womb hypothesis – HanChen Wang – Scientific Reports

Probiotics

Lactobacillus acidophilus, L. plantarum, L. rhamnosus, and L. reuteri Cell-Free Supernatants Inhibit Candida parapsilosis Pathogenic Potential upon Infection of Vaginal Epithelial Cells Monolayer and in a Transwell Coculture System In Vitro – Luca Spaggiari – Microbiology Spectrum

*Probiotic Mixture Containing Lactobacillus helveticus, Bifidobacterium longum and Lactiplantibacillus plantarum Affects Brain Responses Toward an Emotional Task in Healthy Subjects: A Randomized Clinical Trial – Julia Rode – Frontiers in Nutrition

Vaginal microbiome

Identification and characterization of novel endolysins targeting Gardnerella vaginalis biofilms to treat bacterial vaginosis – Sara Arroyo-Moreno – npj Biofilms and Microbiomes

Gut microbiome

*Review: Deciphering mechanisms and implications of bacterial translocation in human health and disease – Shen Jin – Current Opinion in Microbiology

Two Competing Guilds as a Core Microbiome Signature for Chronic Diseases – Guojun Wu – bioRxiv

Neonatal Immune Challenge influences the Microbiota and Behaviour in a Sexually Dimorphic Manner – A. Cuskelly – Brain, Behavior, and Immunity

Review: Diet- and microbiota-related metabolite, 5-aminovaleric acid betaine (5-AVAB), in health and disease – Retu Haikonen – Trends in Endocrinology & Metabolism

Gut mycobiome

Review: “Molding” immunity—modulation of mucosal and systemic immunity by the intestinal mycobiome in health and disease – Mackenzie W. Gutierrez – Nature Mucosal Immunology

Animal experiments

Evolution of the murine gut resistome following broad-spectrum antibiotic treatment – Laura de Nies – Nature Communications & Behind the paper here.

Plant, root/soil microbiome

*Sugars dominate the seagrass rhizosphere – E. Maggie Sogin – Nature Ecology & Evolution

Viruses

A snapshot of the global drinking water virome: Diversity and metabolic potential vary with residual disinfectant use – Bridget Hegarty – Water Research

Techniques

Fast quantification of gut bacterial species in cocultures using flow cytometry and supervised classification – Charlotte C. van de Velde – ISME Communications

Bioinformatics

A deep siamese neural network improves metagenome-assembled genomes in microbiome datasets across different environments – Shaojun Pan – Nature Communications

HiFine: integrating Hi-c-based and shotgun-based methods to reFine binning of metagenomic contigs – Yuxuan Du and Fengzhu Sun – Bioinformatics

happi: a Hierarchical Approach to Pangenomics Inference – Pauline Trinh – bioRxiv

Investigating the impact of database choice on the accuracy of metagenomic read classification for the rumen microbiome – Rebecca H. Smit – bioRxiv

TAMPA: interpretable analysis and visualization of metagenomics-based taxon abundance profiles – Varuni Sarwal – bioRxiv

April 30, 2022

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Human Microbiome

Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa – Octavio Mondragón-Palomino – PNAS

Global, distinctive, and personal changes in molecular and microbial profiles by specific fibers in humans – Samuel M Lancaster – Cell Host & Microbe

Comprehensive human amniotic fluid metagenomics supports the sterile womb hypothesis – HanChen Wang – Scientific Reports

Disordered development of gut microbiome interferes with the establishment of the gut ecosystem during early childhood with atopic dermatitis – Min-Jung Lee – Gut Microbes

A starch- and sucrose-reduced dietary intervention in irritable bowel syndrome patients produced a shift in gut microbiota composition along with changes in phylum, genus, and amplicon sequence variant abundances, without affecting the micro-RNA levels – Clara Nilholm – United European Gastroenterology Journal

Alterations in intestinal microbiota in ultramarathon runners – Mika Sato – Scientific Reports

Lost microbes of COVID-19: Bifidobacterium, Faecalibacterium depletion and decreased microbiome diversity associated with SARS-CoV-2 infection severity – Sabine Hazan – BMJ Open Gastroenterology

Gut microbiota alterations in critically ill older patients: a multicenter study – Mesa Victoria – BMC Geriatrics

Bone marrow transplantation induces changes in the gut microbiota that chronically increase the cytokine response pattern of splenocytes – Saeed Katiraei – Scientific Reports

Gut microbial β-glucuronidases regulate host luminal proteases and are depleted in irritable bowel syndrome – Adam L Edwinson – Nature Microbiology

Review: Communication between the gut microbiota and peripheral nervous system in health and chronic disease – Tyler M Cook – Gut Microbe

Systematic Review: Adverse events in fecal microbiota transplantation: a systematic review and meta-analysis – Eliot A Rapoport – Annals of Gastroenterology

Review: Eco-evolutionary impact of ultraviolet radiation (UVR) exposure on microorganisms, with a special focus on our skin microbiome – Sandhya Rai – Microbiological Research

Beneficial Microbes and Compounds

Review: The huge clinical potential of microbiota in the treatment of pancreatic cancer: The next frontier – Zhilin Zhang – Biochimica et Biophysica Acta. Reviews on Cancer

Oral Administration of Lactobacillus paracasei N1115 on Neonatal Mice Prevents the Intestinal Inflammation in Adulthood – Yiping Xun – Letters in Applied Microbiology

Animal Model Microbiome

ABO genotype alters the gut microbiota by regulating GalNAc levels in pigs – Hui Yang – Nature

External light-dark cycle shapes gut microbiota through intrinsically photosensitive retinal ganglion cells – Chi-Chan Lee – EMBO Reports

Non-Model Animal Microbiome

Host-Associated Bacterial Communities Vary Between Daphnia galeata Genotypes but Not by Host Genetic Distance – Amruta Rajarajan – Microbial Ecology

Faecal Microbiota Divergence in Allopatric Populations of Podarcis lilfordi and P. pityusensis, Two Lizard Species Endemic to the Balearic Islands – Iris Alemany – Microbial Ecology

Gut microbiota of ring-tailed lemurs (Lemur catta) vary across natural and captive populations and correlate with environmental microbiota – Sally L Bornbusch – Animal Microbiome

Mechanisms of fungal community assembly in wild stoneflies moderated by host characteristics and local environment – Yu-Xi Zhu – NPJ Biofilms and Microbiomes

Changes to the gut microbiota of a wild juvenile passerine in a multidimensional urban mosaic – Öncü Maraci – Scientific Reports

Comparative study on gut microbiota in three Anura frogs from a mountain stream – Zhuo Chen – Ecology and Evolution

Plant Microbiome

Insights into the microbiome assembly during different growth stages and storage of strawberry plants – Expedito Olimi – Environmental Microbiome

More Microbes, Genes, and Proteins

Hypothesis: Does Lactobacillus reuteri influence ergothioneine levels in the human body? – Irwin K Cheah – FEBS Letters

Type VI secretion systems of pathogenic and commensal bacteria mediate niche occupancy in the gut – Antonio Serapio-Palacios – Cell Reports

Review: Utilization of glycosaminoglycans by the human gut microbiota: participating bacteria and their enzymatic machineries – Parkash Singh Rawat – Gut Microbes

Bioinformatics

DEPP: Deep Learning Enables Extending Species Trees using Single Genes – Yueyu Jiang – Systematic Biology

A deep siamese neural network improves metagenome-assembled genomes in microbiome datasets across different environments – Shaojun Pan – Nature Communications

Compositionally Aware Phylogenetic Beta-Diversity Measures Better Resolve Microbiomes Associated with Phenotype – Cameron Martino – mSystems

Review: Could Artificial Intelligence/Machine Learning and Inclusion of Diet-Gut Microbiome Interactions Improve Disease Risk Prediction? Case Study: Coronary Artery Disease – Baiba Vilne – Frontiers in Microbiology

Review: Microbial dark matter: from discovery to applications – Yuguo Zha – Genomics, Proteomics & Bioinformatics

April 8, 2022

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Good morning! Today’s microbiome digest is brought to you by mild insomnia induced by a mid-afternoon coffee. It includes two food related studies, one on pathogenic E. coli found in ready-to-eat foods and the other focused on a antifungal lipopeptide produced by B. velezensis isolated from honey, and an an article focused on a new approach for processing frozen lung and lavage samples for use in microbiome studies. Also included are several articles focused on the gut microbiome, and several preprints focused on soil microbiome studies. Finally, I want to highlight a piece that details the positive outcomes associated with the National Summer Undergraduate Research Project (NSURP). Registration for this year’s program is still open until April 15th, and the organizers are looking for both mentors, mentees, and donors!


Pregnancy and early life

Group B Streptococcus colonization at delivery is associated with maternal peripartum infection – Brigtsen et al. – PLOS ONE

Human gut microbiome

Mycobacterium avium subsp. paratuberculosis and microbiome profile of patients in a referral gastrointestinal diseases centre in the Sudan – Elmagzoub et al. – PLOS ONE

Animal experiments

Vertical transmission of attaching and invasive E. coli from the dam to neonatal mice predisposes to more severe colitis following exposure to a colitic insult later in life – Brand et al. – PLOS ONE

Sex differences in the fecal microbiome and hippocampal glial morphology following diet and antibiotic treatment – Saxena et al. – PLOS ONE

Animal microbiome

Preprint: Anthropogenic disturbance impacts gut microbiome homeostasis in a Malagasy primate – Wasimuddin et al. – bioRxiv

Plant, root, and soil microbiome

Preprint: Microclimate is a strong predictor of the native and invasive plant-associated soil microbiota on San Cristóbal Island, Galápagos archipelago – Schoenborn et al. bioRxiv

Preprint: The activity and functions of soil microbial communities in the Finnish sub-Arctic vary across vegetation types – Vitamaki et al. – bioRxiv

Preprint: Tracing carbon metabolism with stable isotope metabolomics reveals the legacy of diverse carbon sources in soil – Wilhelm et al. – bioRxiv

Water and extremophile microbiome (sea, rivers, lakes, glaciers, permafrost, high-salt)

Preprint: Culexarchaeia, a novel archaeal class of anaerobic generalists inhabiting geothermal environments – Kohtz et al. – bioRxiv

Food microbiology

Processed ready-to-eat (RTE) foods sold in Yenagoa Nigeria were colonized by diarrheagenic Escherichia coli which constitute a probable hazard to human health – Beshiru et al. – PLOS ONE

Purification and characterization of antifungal lipopeptide produced by Bacillus velezensis isolated from raw honey – Ziong et al. – PLOS ONE

Techniques

An optimized approach for processing of frozen lung and lavage samples for microbiome studies – Wiscovitch-Russo et al. – PLOS ONE

Science, publishing, and career

***National Summer Undergraduate Research Project (NSURP): A Virtual Research Experience to Deliver REAL (Retention, Equity, Access, and Life-Changing) Outcomes for Underrepresented Minorities in STEM – Johnson & Knox – JMBE

March 30, 2022

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General microbiome

[REVIEW]Glycan processing in gut microbiomes – La Rosa et al. – Current Opinion in Microbiology

Multi-site human microbiome

[REVIEW]The Oral-Microbiome-Brain Axis and Neuropsychiatric Disorders: An Anthropological Perspective – Bowland & Weyrich – Frontiers in Psychiatry

Human oral microbiome

Microbial and molecular differences according to the location of head and neck cancers – Kim et al. – Cancer Cell International

Microbial differences between active and remission peri-implantitis – Hashimoto et al. – Scientific Reports

Human gut microbiome

Dynamics of the normal gut microbiota: A longitudinal one-year population study in Sweden – Olsson et al. – Cell Host & Microbe

Microbiota-microglia connections in age-related cognition decline – Zhou et al. – Aging Cell

Patients with psoriasis have a dysbiotic taxonomic and functional gut microbiota – Todberg – British Journal of Dermatology

[PREPRINT]Distinct Colon Mucosa Microbiomes associated with Tubular Adenomas and Serrated Polyps – Avelar-Barragan et al. – bioRxiv

Early prediction of incident liver disease using conventional risk factors and gut-microbiome-augmented gradient boosting – Liu et al. – Cell Metabolism

Insights from shotgun metagenomics into bacterial species and metabolic pathways associated with NAFLD in obese youth – Testerman et al. – Hepatology Commonications

Dietary Patterns and Gut Microbiota: the Crucial Actors in Inflammatory Bowel Disease – He et al. – Advances in Nutrition

A Guide to Dietary Pattern–Microbiome Data Integration – Choi et al. – The Journal of Nutrition

Functional Significance of Different Milk Constituents in Modulating the Gut Microbiome and Infant Health – Yadav et al. – J. Agric. Food Chem.

The gut microbiome as mediator between diet and its impact on immune function – Shi et al. – Scientific Reports

Differences in the intestinal microbiome associated with diarrhea during lenvatinib treatment for hepatocellular carcinoma – Inuai et al. – Digestive Diseases

16S rDNA sequencing analyzes differences in intestinal flora of human immunodeficiency virus (HIV) patients and association with immune activation – Mingjun et al. – Bioengineered

[REVIEW]Profile of Gut Microbiota of Adults with Diabetes Mellitus Type 1: A Systematic Review – Moreira et al. – Current Diabetes Reviews

Association between systemic lupus erythematosus and disruption of gut microbiota: a meta-analysis – Xiang et al. – Immunology and Inflammation

[REVIEW]The Bridge Between Ischemic Stroke and Gut Microbes: Short-Chain Fatty Acids – Fang et al. – Cellular and Molecular Neurobiology

Animal experiments (mouse/rat experiments, chickens/pigs on different diets)

Prophylactic effects of hyperforin on anhedonia-like phenotype in chronic restrain stress model: A role of gut microbiota – Zhang et al. – Letters in Applied Microbiology

Metabolome and microbiome multi-omics integration from a murine lung inflammation model of bronchopulmonary dysplasia – Saie et al. – Pediatric Research

Microbiome applications for laying hen performance and egg production – Ricke, Dittoe & Olson – Poultry Science

Animal microbiome (for animals in the wild)

[PREPRINT]The connection between the gut mycobiome community of natural black soldier fly populations and their environment – Vitenberg, Goldway & Opatovsky – bioRxiv

Inhibitory Bacterial Diversity and Mucosome Function Differentiate Susceptibility of Appalachian Salamanders to Chytrid Fungal Infection – Jimenez et al. – Applied and Environmental Microbiology

Composition and short-term stability of gut microbiota in lean and spontaneously overweight healthy Labrador retriever dogs – Söder et al. – Acta Veterinaria Scandinavica

Alterations in common marmoset gut microbiome associated with duodenal strictures – Sheh et al. – Scientific Reports

Plant, root, and soil microbiome

Spatial organization of the kelp microbiome at micron scales – Ramirez-Puebla et al. – Microbiome

Water and extremophile microbiome (sea, rivers, lakes, glaciers, permafrost, high-salt)

Metagenomics reveals global-scale contrasts in nitrogen cycling and cyanobacterial light-harvesting mechanisms in glacier cryoconite – Marakami et al. – Microbiome

Bacterial communities in surface and basal ice of a glacier terminus in the headwaters of Yangtze River on the Qinghai–Tibet Plateau – Ren et al. – Envionmental Microbiome

Built environment (subways, indoor surfaces)

Multikingdom interactions govern the microbiome in subterranean cultural heritage sites – Liu et al. – PNAS

Indoor green can modify the indoor dust microbial communities – Dockx et al. – Indoor Air

Forensics

Microbial community succession of submerged bones in an aquatic habitat – Kaszubinski et al . – Journal of Forensic Sciences

Bioinformatics

Metagenomic strain detection with SameStr: identification of a persisting core gut microbiota transferable by fecal transplantation – Podlesny et al. – Microbiome

[PREPRINT]Detection of Parasites in Microbiomes using Metagenomics – Kirstagler, Aarestrup & Pamp – bioRxiv

Techniques

Tryptophan Supplementation Increases the Production of Microbial-Derived AhR Agonists in an In Vitro Simulator of Intestinal Microbial Ecosystem – Koper et al. – J. Agric. Food Chem.

March 23, 2022

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General microbiome

Microbial communities form rich extracellular metabolomes that foster metabolic interactions and promote drug tolerance – Yu et al. – Nature Microbiology

Human skin microbiome

Skin microbiota diversity among genetically unrelated individuals of Indian origin – Pothbhare et al. – PeerJ

Human gut microbiome

[REVIEW]Gut microbiota and allergic diseases in children – Akagawa & Kaneko – Allergology International

[REVIEW]Gut microbiome: New biomarkers in early screening of colorectal cancer – Zhou et al. – Journal of Clinical Laboratory Analysis

Can gut microbiota throughout the first 10 years of life predict executive functioning in childhood? – Eckermann et al. – Developmental Psychobiology

[PREPRINT]Meta-analysis of the autism gut microbiome identifies factors influencing study discrepancies and machine learning classification – Chavira, Wang & Mills – bioRxiv

Changes of Intestinal Microflora in Colorectal Cancer Patients after Surgical Resection and Chemotherapy – Huang et al. – Computational and Mathematical Methods in Medicine

Animal microbiome

Captivity and Animal Microbiomes: Potential Roles of Microbiota for Influencing Animal Conservation – Dallas & Warne – Microbial Ecology

Changes in gut microbiota composition with age and correlations with gut inflammation in rats – Meng et al. – PLOS One

[REVIEW]Translational multi-omics microbiome research for strategies to improve cattle production and health – Wang & Guan – Emerging Topics in Lefe Sciences

Multi-omics reveals host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine – Zhang et al. – Food & Function

Soil causes gut microbiota to flourish and total serum IgE levels to decrease in mice – Zhou et al. – Environmental Microbiology

Microbiome-metabolomics deciphers the effects of Cistanche deserticola polysaccharides on agedconstipated rats – Liu et al. – Food & Function

Plant, root, and soil microbiome

[PREPRINT]Plant Virus Database: a resource for exploring the diversity of plant viruses and their interactions with hosts – Wu et al. – bioRxiv

Waste microbiome

[PREPRINT]Identification of Microbial Flora and Isolation of Functional Strains Related to the Degradation of Domestic Waste – Huang, Liu & Zong – bioRxiv

Food microbiology

Comprehensive characterization of the bacterial community structure and metabolite composition of food waste fermentation products via microbiome and metabolome analyses – Li et al. – PLOS One

Bioinformatics

MetaPop: a pipeline for macro- and microdiversity analyses and visualization of microbial and viral metagenome-derived populations – Gregory et al. – Microbiome

HAM-ART: An optimised culture-free Hi-C metagenomics pipeline for tracking antimicrobial resistance genes in complex microbial communities – Kalmar et al. – PLOS Genetics

Techniques

[PREPRINT]Vaginal microbiome-host interactions modeled in a human vagina-on-a-chip – Mahajan et al. – bioRxiv

Microbes in the news (popular press, EurekAlert, ScienceDaily, Youtube, NPR, etc)

Florence tomb by Michelangelo restored with aid of bacteria – The Guardian

March 09, 2022

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General microbiome

Public human microbiome data are dominated by highly developed countries – Abdill, Adamowicz & Blekhman – PLOS Biology

Beyond the Microbiome: Germ-ganism? An Integrative Idea for Microbial Existence, Organization, Growth, Pathogenicity, and Therapeutics – Kambouris et al. – OMICS

Quantifying and Cataloguing Unknown Sequences within Human Microbiomes – Modha et al. – mSystems

Human respiratory microbiome

Alterations of lung microbiota in patients with non-small cell lung cancer – Zeng et al. – Bioengineered

Human vaginal microbiome

Evaluation of microbiome enrichment and host DNA depletion in human vaginal samples using Oxford Nanopore’s adaptive sequencing – Marquet et al. – Scientific Reports

Human gut microbiome

[REVIEW]Personal diet–microbiota interactions and weight loss – Roager & Christensen

Integrated analysis of gut microbiome and host immune responses in COVID-19 – Xu et al. – Frontiers in Medicine

Animal experiments (mouse/rat experiments, chickens/pigs on different diets)

Gut microbiome modifications over time when removing in-feed antibiotics from the prophylaxis of post-weaning diarrhea in piglets – Cremonesi et al. – PLOS One

Complete Genome Sequences of Three Staphylococcus haemolyticus Strains Isolated from the Lung of a TGFβ1 Transgenic Mouse with Lung Fibrosis – Abdel-Hamid et al. – Microbiology Resource Announcements

Colitis-associated microbiota drives changes in behaviour in male mice in the absence of inflammation – Vicentini et al. – Brain, Behaviour, and Immunity

Comprehensive mouse microbiota genome catalog reveals major difference to its human counterpart – Kieser, Zdobnov & Trajkovski – PLOS Comp Biol

Plant, root, and soil microbiome

The microbiome and resistome of apple fruits alter in the post-harvest period – Wassermann et al. – Environmental Microbiome

Water and extremophile microbiome (sea, rivers, lakes, glaciers, permafrost, high-salt)

Effects of Ocean Warming on the Underexplored Members of the Coral Microbiome – Maire et al. – Integrative and Comparative Biology

Techniques

[PREPRINT]A comparison of six DNA extraction protocols for 16S, ITS, and shotgun metagenomic sequencing of microbial communities – Shaffer et al. – bioRxiv

[PREPRINT]A comparison of hard and soft direct methods for DNA extraction from soil – Hill et al. – bioRxiv

March 3, 2022

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Today’s digest is heavy on studies focused on the gut and soil microbiomes. It also includes articles focused on microbial indoor air quality as well as bacterial vaginosis and aerobic vaginitis among pregnant women. If you’d still like more, I’ve also included several preprints.

Happy reading!


Human vaginal microbiome

Prevalence of bacterial vaginosis and aerobic vaginitis and their associated risk factors among pregnant women from northern Ethiopia: A cross-sectional study – Yalew et al. – PLOS One

Human gut microbiome

Systematically assessing microbiome–disease associations identifies drivers of inconsistency in metagenomic research – Tierney et al. – PLOS Biology

Preprint: Gut microbiome dysbiosis during COVID-19 is associated with increased risk for bacteremia and microbial translocation – Venzon et al. – bioRxiv

Hydrogen-producing small intestinal bacterial overgrowth is associated with hepatic encephalopathy and liver function – Yokoyama et al. – PLOS One

Plant, root, and soil microbiome

Preprint: Deciphering the potential niche of two novel black yeast fungi from a biological soil crust based on their genomes, phenotypes, and melanin regulation – Carr et al. – bioRxiv

Bacterial age distribution in soil—Generational gaps in adjacent hot and cold spots – Borer & Or – PLOS Computational Biology

Deciphering the influence of Bacillus subtilis strain Ydj3 colonization on the vitamin C contents and rhizosphere microbiomes of sweet peppers – Liang et al. – PLOS One

Antimicrobial effect and mechanism of bovine lactoferrin against the potato common scab pathogen Streptomyces scabiei – Nakamura et al. – PLOS One

Preprint: Arbuscular mycorrhizal fungal composition across US citrus orchards, management strategies, and disease severity spectrum – Xi et al. – bioRxiv

Built environment

Comprehensive health risk assessment of microbial indoor air quality in microenvironments – Kumar et al. – PLOS One

Phages and viruses

Preprint: Modelling the synergistic effect of bacteriophage and antibiotics on bacteria: killers and drivers of resistance evolution – Leclerc et al. -bioRxiv

Bioinformatics

Preprint: Structure-informed microbial population genetics elucidate selective pressures that shape protein evolution – Kiefl et al. – bioRxiv

February 9, 2022

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Today’s digest includes a Nature Medicine news feature on the role of preprints in the COVID-19 pandemic. Also included are an article about unsupervised clustering methods for microbiome data, the response of anammox consortia to nutrient starvation, and a handful of interesting preprints.

Animal experiments

Preprint: Phylogenetic diversity analysis of shotgun metagenomic reads describes gut microbiome development and treatment effects in the post-weaned pig – Gaio et al. – bioRxiv

Plant, root, and soil microbiome

Preprint: Ecological insights into soil health according to the life-history traits and environment-wide associations of bacteria in agricultural soils – Wilhelm et al. – bioRxiv

Water and extremophile microbiome

Response and resilience of anammox consortia to nutrient starvation – Wang et al. – Microbiome

Bioinformatics

Preprint: Model-free prediction of microbiome compositions – Asher & Bashan – bioRxiv

Preprint: Predicting the re-distribution of antibiotic molecules caused by inter-species interactions in microbial communities – Reding – bioRxiv

Performance determinants of unsupervised clustering methods for microbiome data – Shi et al. – Microbiome

Science, publishing, and career

Rise of the preprint: how rapid data sharing during COVID-19 has changed science forever – Watson – Nature Medicine

February 4, 2022

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Today’s digest is super diverse (read cool!): novel genes from uncultivated prokaryotes, a new Latilactobacillus species isolated from leaves of a strawberry plant (read the full 🍓 story behind the paper!), the benchmarking study of taxonomic profilers for long-read shotgun metagenomic datasets, and more. The first Issue of a new international peer-reviewed, open access, microbiome-oriented journal – the Microbiome Research Reports – is out with one original article, one perspective and four reviews.
And, a bit of shameless self-promotion, check out my preprint on urinary microbiome of healthy European women!
Happy Friday!

Global microbiome

*Functional and evolutionary significance of unknown genes from uncultivated taxa – Alvaro Rodríguez – bioRxiv

Ear microbiome

The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes – Maria Burton – PlosOne

Respiratory microbiome

Intestinal Akkermansia muciniphila predicts clinical response to PD-1 blockade in patients with advanced non-small-cell lung cancer – Lisa Derosa – Nature Medicine

Associations and recovery dynamics of the nasopharyngeal microbiota during influenza‑like illness in the aging population – Sudarshan A. Shetty – Scientific Reports

Genomic diversity and antimicrobial resistance of Prevotella species isolated from chronic lung disease airways – Kasey A. Webb – Microbial Genomics

Gut microbiome

Effect of host genetics on the gut microbiome in 7,738 participants of the Dutch Microbiome Project – Esteban A. Lopera-Maya – Nature Genetics


The gut bacterial community potentiates Clostridioides difficile infection severity – Nicholas A Lesniak – bioRxiv

Emergent evolutionary forces in spatial models of microbial growth in the human gut microbiota – Olivia M. Ghosh and Benjamin H. Good – bioRxiv

Review: Microbiome-based therapeutics – Matthew T. Sorbara and Eric G. Pamer – Nature Reviews Microbiology

Review: Multimodal interactions of drugs, natural compounds and pollutants with the gut microbiota –Anna E. Lindell – Nature Reviews Microbiology

Review: Gut microbiome and health: mechanistic insights – Willem M de Vos – Gut

Vaginal microbiome

Gardnerella vaginalis induces matrix metalloproteinases in the cervicovaginal epithelium through TLR-2 activation – Kristin D. Gerson – American Journal of Obstetrics & Gynecology/SMFM 42nd Annual Meeting: The Pregnancy Meeting

Urinary microbiome

*Extended bacterial diversity of the urinary microbiome of reproductive-age healthy European women captured by culturomics and long-read amplicon sequencing – Svetlana Ugarcina Perovic – bioRxiv

Phylogenetic analysis and antimicrobial susceptibility profile of uropathogens – Hanif Ullah – PlosOne

Multidrug-resistant Uro-associated Escherichia coli Populations and Recurrent Urinary Tract Infections in Patients Performing Clean Intermittent Self-catheterisation – Catherine Mowbray – European Urology Open Science

Plant microbiome

*Latilactobacillus fragifolii sp. nov., isolated from leaves of a strawberry plant (Fragaria x ananassa) – Marie Legein – International Journal of Systematic and Evolutionary Microbiology

Ecology and potential functions of plant-associated microbial communities in cold environments – Malek Marian – FEMS Microbiology Ecology

Animal microbiome

Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides – Lucelia Cabral – Nature Communications

Review: Pesticide-induced disturbances of bee gut microbiotas – Michelle Z Hotchkiss – FEMS Microbiology Reviews

Animal experiments

Effect of a freeze-dried coffee solution in a high-fat diet-induced obesity model in rats: Impact on inflammatory response, lipid profile, and gut microbiota – Marilia Hermes Cavalcanti – PlosOne

Gut microbiota and butyrate contribute to nonalcoholic fatty liver disease in premenopause due to estrogen deficiency – Limin Liu – PlosOne

Techniques

BIOME-Preserve: A novel storage and transport medium for preserving anaerobic microbiota samples for culture recovery – Embriette R. Hyde – PlosOne

Species-targeted sorting and cultivation of commensal bacteria from the gut microbiome using flow cytometry under anaerobic conditions – Samuel Bellais – Microbiome

Bioinformatics

*Evaluation of taxonomic profiling methods for long-read shotgun metagenomic sequencing datasets – Daniel M Portik – bioRxiv

AGAMEMNON: an Accurate metaGenomics And MEtatranscriptoMics quaNtificatiON analysis suite – Giorgos Skoufos – Genome Biology

McComedy: A user-friendly tool for next-generation individual-based modeling of microbial consumer-resource systems – André Bogdanowski – Plos Computational Biology

VeChat: Correcting errors in long reads using variation graphs – Xiao Luo – bioRxiv

ReadBouncer: Precise and Scalable Adaptive Sampling for Nanopore Sequencing – Jens-Uwe Ulrich – bioRxiv

📻

January 28, 2022

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Human and General Microbiome

Diet leaves a genetic signature in a keystone member of the gut microbiota – Tanja Dapa – Cell Host & Microbe

Multi-omics investigation of Clostridioides difficile-colonized patients reveals pathogen and commensal correlates of C. difficile pathogenesis – Skye Rs Fishbein – eLife

Large-scale characterization of the macrolide resistome reveals high diversity and several new pathogen-associated genes – David Lund – Microbial Genomics

The small bowel microbiome changes significantly with age and aspects of the ageing process – Gabriela Leite – Microbial Cell

Intestinal microbiota profiles in a genetic model of colon tumorigenesis correlates with colon cancer biomarkers – Francesco Vitali – Scientific Reports

Skin microbiome profile of healthy Cameroonians and Japanese – Kazuhiro Ogai – Scientific Reports

Interactions between human milk oligosaccharides, microbiota and immune factors in milk of women with and without mastitis – Irma Castro – Scientific Reports

Microbiota of the prostate tumor environment investigated by whole-transcriptome profiling – Paul Vinu Salachan – Genome Medicine

Preview: Microbiome engineering: Taming the untractable – James W Marsh – Cell

Review: Microbiomes in physiology: Insights into 21(st) century global medical challenges – Emad Shehata – Experimental Physiology

Respiratory Tract Microbiome – Highlights

Gut microbiota dynamics in a prospective cohort of patients with post-acute COVID-19 syndrome – Qin Liu – Gut

Characteristics of Lung Microbiota in Children’s Refractory Mycoplasma pneumoniae Pneumonia Coinfected with Human Adenovirus B – Wenxiang Zhou – The Canadian Journal of Infectious Diseases & Medical Microbiology

The lung microbiota in children with cystic fibrosis captured by induced sputum sampling – Rebecca Weiser – Journal of Cystic Fibrosis 

Beneficial Microbes and Compounds

Bifidobacterium animalis subsp. lactis BB-12 Has Effect Against Obesity by Regulating Gut Microbiota in Two Phases in Human Microbiota-Associated Rats – Kemin Mao – Frontiers in Nutrition

Effects of a 10-Strain Oral Probiotic on Parameters of Vaginal Health and Microbial Community: A Pilot Clinical Study – Christopher J Martoni – International Journal of Women’s Health

A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of the gut microbiota and relief from chronic constipation – Luyao Wang – Food & Function

Vaginal states: detection of conversion processes in women using contraception and characterization of vaginal Lactobacillus species – Sonia Elena María Fosch – Infectious Disorders Drug Targets

The probiotic properties and potential of vaginal Lactobacilli spp. isolated from healthy women against some vaginal pathogens – Arezoo Asadi – Letters in Applied Microbiology

Review: Protective Effects of Natural Polysaccharides on Intestinal Barrier Injury: A Review – Jiaying Huo – Journal of Agricultural and Food Chemistry

Animal Microbiome

Nitrogen recycling via gut symbionts increases in ground squirrels over the hibernation season – Matthew D Regan – Science

Robust host source tracking building on the divergent and non-stochastic assembly of gut microbiomes in wild and farmed large yellow croaker – Jun Zhu – Microbiome

Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis – Muhamad Firdaus Syahmi Sam-On – Microbial Pathogenesis

Gut Microbial Trimethylamine is Elevated in Alcohol-Associated Hepatitis and Contributes to Ethanol-Induced Liver Injury in Mice – Robert N Helsley – eLife

Intestinal gluconeogenesis shapes gut microbiota, fecal and urine metabolome in mice with gastric bypass surgery – Justine Vily-Petit – Scientific Reports

Food Microbiome

To culture or not to culture: careful assessment of metabarcoding data is necessary when evaluating the microbiota of a modified-atmosphere-packaged vegetarian meat alternative throughout its shelf-life period – E Duthoo – BMC Microbiology

Plant Microbiome

Root Morphogenesis of Arabidopsis thaliana Tuned by Plant Growth-Promoting Streptomyces Isolated From Root-Associated Soil of Artemisia annua – Wenbo Fu – Frontiers in Plant Science

Environmental Microbiome

Soil microbial community development across a 32-year coastal wetland restoration time series and the relative importance of environmental factors – Katherine M Abbott – The Science of the Total Environment

Emerging frontiers in microbe mediated pesticide remediation: Unveiling role of omics and In silico approaches in engineered environment – Muneer Ahmad Malla – Environmental Pollution 

Review: Emerging investigator series: microplastic sources, fate, toxicity, detection, and interactions with micropollutants in aquatic ecosystems – a review of reviews – Mohamed Ateia – Environmental Science. Processes & Impacts

Techniques

Alternating Dual-Collision Energy Scanning Mass Spectrometry Approach: Discovery of Novel Microbial Bile-Acid Conjugates – Quan-Fei Zhu – Analytical Chemistry

Genetic tools for the redirection of the central carbon flow towards the production of lactate in the human gut bacterium Phocaeicola (Bacteroides) vulgatus – Rebecca Lück – Applied Microbiology and Biotechnology

Lipidomics Analysis of Outer Membrane Vesicles and Elucidation of the Inositol Phosphoceramide Biosynthetic Pathway in Bacteroides thetaiotaomicron – Mariana G Sartorio – Microbiology Spectrum