February 1, 2023

General microbiome

Multi-dimensional experimental and computational exploration of metabolism pinpoints complex probiotic interactions – Zampieri, Efthimiou & Angione – Metabolic Engineering

[REVIEW]Advancing CAR T cell therapy through the use of multidimensional omics data – Yamg et al. – Nature Reviews Clinical Oncology

Pregnancy and early life

[REVIEW]Microbial transmission, colonisation and succession: from pregnancy to infancy – Xiao & Zhai – Gut

Human gut microbiome

Changes in the Gut Microbiome Associated with Intussusception in Patients with Peutz-Jeghers Syndrome – Wang et al. – Microbiology Spectrum

[PREPRINT]Probiotics supplementation to adult human small intestinal stoma microbiota causes dynamic increase in the community resistance to perturbations and nutrient utilization – Jansma et al. – bioRxiv

Animal experiments (mouse/rat experiments, chickens/pigs on different diets)

Pathometagenomics reveals susceptibility to intestinal infection by Morganella to be mediated by the blood group-related B4galnt2 gene in wild mice – Vallier et al. – Gut Microbes

Host-microbe co-metabolism via MCAD generates circulating metabolites including hippuric acid – Pruss et al. – Nature Communications

Disruption of the Intestinal Mucosal Barrier Induced by High Fructose and Restraint Stress Is Regulated by the Intestinal Microbiota and Microbiota Metabolites – Yu et al. – Microbiology Spectrum

[PREPRINT]Shift from a Simplified to Complex Gut Microbiota Reduces Adenoma Burden in a Preclinical Rat Model of Colon Cancer – Busi et al. – bioRxiv

Water and extremophile microbiome (sea, rivers, lakes, glaciers, permafrost, high-salt)

Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms – Vincent et al. – Nature Communications

High-resolution metagenomic reconstruction of the freshwater spring bloom – Kavagutti et al. – Microbiome


[PREPRINT]Accounting for 16S rRNA copy number prediction uncertainty and its implications in microbial diversity analyses – Gao & Wu – bioRxiv


[PREPRINT]Methods for the analysis of skin microbiomes: a comparison of sampling processes and 16S rRNA hypervariable regions – Alyami et al. – bioRxiv

January 18, 2023

Human oral microbiome

[PRIMER]A possible role for taste receptor cells in surveying the oral microbiome – Heisey & McCluskey – PLOS Biology

Type II taste cells participate in mucosal immune surveillance – Qin et al. – PLOS One

Human gut microbiome

[NEWS]How our microbiome is shaped by family, friends and even neighbours – Callaway – Nature

The person-to-person transmission landscape of the gut and oral microbiomes – Valles-Colomer et al. – Nature

[REVIEW]The gut microbiota in obesity and weight management: microbes as friends or foe? – van Hul & Cani – Nature Reviews Endocrinology

Dynamics of the gut microbiome, IgA response, and plasma metabolome in the development of pediatric celiac disease – Girdhar et al. – Microbiome

Multi-omics profiles of the intestinal microbiome in irritable bowel syndrome and its bowel habit subtypes – Jacobs et al. – Microbiome

Microbiome Features Differentiating Unsupervised-Stratification-Based Clusters of Patients with Abnormal Glycometabolism – Xu et al. – mBio

Gut Bacterial Dysbiosis in Irritable Bowel Syndrome: a Case-Control Study and a Cross-Cohort Analysis Using Publicly Available Data Sets – Kim, Lee & Shim – Microbiology Spectrum

Harnessing gut microbes for glycan detection and quantification – Modesto, Pearce & Townsend Il – Nature Communications

The gut-microbiota-brain axis in a Spanish population in the aftermath of the COVID-19 pandemic: microbiota composition linked to anxiety, trauma, and depression profiles – Malan – Müller et al. – Gut Microbes

Animal microbiome

Expanded catalogue of metagenome-assembled genomes reveals resistome characteristics and athletic performance-associated microbes in horse – Li et al. – Microbiome

The bovine foot skin microbiota is associated with host genotype and the development of infectious digital dermatitis lesions – Bay et al. – Microbiome

Plant, root, and soil microbiome

A framework for the targeted recruitment of crop-beneficial soil taxa based on network analysis of metagenomics data – Merihu et al. – Microbiome

Diversity and functional analysis of rumen and fecal microbial communities associated with dietary changes in crossbreed dairy cattle – Kibegwa et al. – PLOS One


Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Assessing reference database, variable region and pre-processing bias in classification of mock bacterial community samples – Maki et al. – PLOS One

December 12, 2022

Today’s digest has the kind of articles I like best–they are all focused on the unique relationship between microbial communities and their niche environments. These environments span everything from rice seeds to the Mariana Trench to the gut microbiome and even Appalachian coalbeds. Happy reading!

Human gut microbiome

Gut microbiome of helminth-infected indigenous Malaysians is context dependent – Tee et al. – Microbiome

Animal experiments

Taurine metabolism is modulated in Vibrio-infected Penaeus vannamei to shape shrimp antibacterial response and survival – Wang et al. – Microbiome

Plant, root, and soil microbiome

Multi-genome metabolic modeling predicts functional inter-dependencies in the Arabidopsis root microbiome – Mataigne et al. – Microbiome

Dynamics of rice microbiomes reveal core vertically transmitted seed endophytes – Zhang et al. – Microbiome

Water and extremophile microbiome

Comparison of prokaryotes between Mount Everest and the Mariana Trench – Liu et al. – Microbiome

Built environment

Predominance of Methanomicrobiales and diverse hydrocarbon-degrading taxa in the Appalachian coalbed biosphere revealed through metagenomics and genome-resolved metabolisms – Ross et al. – Environmental Microbiology

Evaluation of the Microbial Community and Geochemistry in Produced Waters Collected from CO2 EOR in the Niagaran Pinnacle Reef – Stemple et al. – ACS Earth Space Chem

December 12, 2022

Human and General Microbiome

Dental calculus microbiome correlates with dietary intake – Gabriel Innocenti – Molecular Oral Microbiology

Ruminococcus gnavus plays a pathogenic role in diarrhea-predominant irritable bowel syndrome by increasing serotonin biosynthesis – Lixiang Zhai – Cell Host & Microbe

The gut microbiome and child mental health: a population-based study – Robert Kraaij – Brain, Behavior, and Immunity

Gut microbiota profile of COVID-19 patients: Prognosis and risk stratification (MicroCOVID-19 study) – José Guilherme Nobre – Frontiers in Microbiology

Systematic Review: Gut microbiota diversity and composition in predicting immunotherapy response and immunotherapy-related colitis in melanoma patients: A systematic review – Oliver Oey – World Journal of Clinical Oncology

Review: Scientometric analysis of kidney disease and gut microbiota from 2001 to 2020 based on Web of Science – Ran Zhang – Medicine

Review: Crosstalk between liver macrophages and gut microbiota: An important component of inflammation-associated liver diseases – Ziyuan Zhou – Frontiers in Cell and Developmental Biology

Review: Dental calculus – oral health, forensic studies and archaeology: a review – Roger Forshaw – British Dental Journal

Animal Microbiome

Inputs don’t equal outputs: Bacterial microbiomes of the ingesta, gut, and feces of the keystone deposit feeder Ilyanassa obsoleta – Craig J Plante – FEMS Microbiology Ecology

Urinary neopterin reflects immunological variation associated with age, helminth parasitism, and the microbiome in a wild primate – India A Schneider-Crease – Scientific Reports

Dark-purple rice extract modulates gut microbiota composition in acetic acid- and indomethacin-induced inflammatory bowel disease in rats – Kornsuda Thipart – International Microbiology

Review: Nasonia-microbiome associations: a model for evolutionary hologenomics research – Zhengyu Zhu – Trends in Parasitology

Microbiome Analysis

Rapid separation of bacteria from primary nasal samples using inertial microfluidics – Jesus Shrestha – Lab on a Chip

November 8, 2022

Greetings from Pittsburgh, PA!

It’s election day here, which means I have low grade nausea and am looking for a distraction. If you’re in the same boat, check out today’s awesome articles on a holobiont deep sea shrimp, plant-microbiome assemblies of poplar trees, and viral community-wide auxiliary metabolic genes. I also included a few interesting preprints, my favorite of which is noted below and uses a microbiome public dataset to compare different normalization and transformation methods.

Animal microbiome

A novel and dual digestive symbiosis scales up the nutrition and immune system of the holobiont Rimicaris exoculata – Aubé et al. – Microbiome

Plant, root, and soil microbiome

Plant sex affects plant-microbiome assemblies of dioecious Populus cathayana trees under different soil nitrogen conditions – Guo et al. – Microbiome

Phages and viruses

Viral community-wide auxiliary metabolic genes differ by lifestyles, habitats, and hosts – Luo et al. – Microbiome


*Preprint: Alpha and beta-diversities performance comparison between different normalization methods and centered log-ratio transformation in a microbiome public dataset – Bars-Cortina – bioRxiv

Preprint: Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts – McCallum et al. – bioRxiv

Preprint: Targeted metagenomics using probe capture detects a larger diversity of nitrogen and methane cycling genes in complex microbial communities than traditional metagenomics – Siljanen et al. – bioRxiv

October 17, 2022

Human and General Microbiome

Comparison of microbial signatures between paired faecal and rectal biopsy samples from healthy volunteers using next-generation sequencing and culturomics – Indrani Mukhopadhya – Microbiome

Depression and fatigue in active IBD from a microbiome perspective-a Bayesian approach to faecal metagenomics – Anne Kerstin Thomann – BMC Medicine

Dynamics of human milk oligosaccharides in early lactation and relation with growth and appetitive traits of Filipino breastfed infants – Tinu M Samuel – Scientific Reports

The gut microbiome-metabolome dataset collection: a curated resource for integrative meta-analysis – Efrat Muller – NPJ Biofilms and Microbiomes

Review: Strains to go: interactions of the skin microbiome beyond its species – Ryan Caldwell – Current Opinion in Microbiology

Gut bacterial extracellular vesicles: important players in regulating intestinal microenvironment – Xiao Liang – Gut Microbes

Brief overview of dietary intake, some types of gut microbiota, metabolic markers and research opportunities in sample of Egyptian women – Nayera E Hassan – Scientific Reports

Profile of gut microbiota and serum metabolites associated with metabolic syndrome in a remote island most afflicted by obesity in Japan – Tsugumi Uema – Scientific Reports

The intestinal clock drives the microbiome to maintain gastrointestinal homeostasis – Marjolein Heddes – Nature Communications

Identification of bacterial lipopeptides as key players in IBS – Camille Petitfils – Gut

Gut-innervating nociceptors regulate the intestinal microbiota to promote tissue protection – Wen Zhang – Cell

BefA, a microbiota-secreted membrane disrupter, disseminates to the pancreas and increases β cell mass – Jennifer Hampton Hill – Cell Metabolism

Alterations in the nasopharyngeal microbiome associated with SARS-CoV-2 infection status and disease severity – Nick P G Gauthier – PloS one

preprint: Ultra-deep Sequencing of Hadza Hunter-Gatherers Recovers Vanishing Gut Microbes – Bryan D Merrill – bioRxiv

Beneficial Microbes and Compounds

Long-term use of Lacticaseibacillus paracasei N1115 from early life alleviates high-fat-diet-induced obesity and dysmetabolism in mice – Z H Miao – Beneficial Microbes

Animal Model Microbiome

Trimethylamine, a gut bacteria metabolite and air pollutant, increases blood pressure and markers of kidney damage including proteinuria and KIM-1 in rats – Klaudia M Maksymiuk – Journal of Translational Medicine

Animal Microbiome

Molecular detection of Coxiella-like endosymbionts and absence of Coxiella burnetii in Amblyomma mixtum from Veracruz, Mexico – Estefanía Grostieta – Experimental & Applied Acarology

Environmental Microbiome

An enriched ammonia-oxidizing microbiota enables high removal efficiency of ammonia in antibiotic production wastewater – Jiamin Chen – Chemosphere

October 14, 2022

Today’s digest features manipulation of crop microbiome to minimise impact of climate change on agricultural productivity, turtle microbiome, #microber2022, and more. Happy Friday!

See you next week at #MVIF!

Human microbiome

Review: Microbiome epidemiology and association studies in human health – Hannah VanEvery – Nature Reviews Genetics

Gut microbiome

The effects of fermented vegetable consumption on the composition of the intestinal microbiota and levels of inflammatory markers in women: A pilot and feasibility study – Amy E. Galena – PlosOne

Functional screens of barcoded expression libraries uncover new gene functions in carbon utilization among gut Bacteroidales – Yolanda Y. Huang – bioRxiv

Advancing human gut microbiota research by considering gut transit time – Nicola Procházková – Gut

Food Additive Emulsifiers and Their Impact on Gut Microbiome, Permeability, and Inflammation: Mechanistic Insights in Inflammatory Bowel Disease – Aaron S Bancil – Journal of Crohn’s and Colitis

Influence of the microbiome, diet and genetics on inter-individual variation in the human plasma metabolome – Lianmin Chen – Nature Medicine

Vaginal microbiome

N-glycosylation of cervicovaginal fluid reflects microbial community, immune activity, and pregnancy status – Gang Wu – Scientific Reports

Animal microbiome

*More than just hitchhikers: a survey of bacterial communities associated with diatoms originating from sea turtles Get access Arrow – Klara Filek – FEMS Microbiology Ecology

Plant microbiome

*Next generation tools for crop-microbiome manipulation to mitigate the impact of climate change – Bruna D. Batista & Brajesh K. Singh – Environmental Microbiology

Soil microbiome

Defending Earth’s terrestrial microbiome – Colin Averill – Nature Microbiology


GTDB-Tk v2: memory friendly classification with the Genome Taxonomy Database – Pierre-Alain – Bioinformatics

binny: an automated binning algorithm to recover high-quality genomes from complex metagenomic datasets – Oskar Hickl – bioRxiv

VEBA: a modular end-to-end suite for in silico recovery, clustering, and analysis of prokaryotic, microeukaryotic, and viral genomes from metagenomes – Josh L. Espinoza & Chris L. Dupont – BMC Bioinformatics

September 22, 2022

Happy almost Friday! Today’s digest includes a time series tracking changes after fecal transplant, biofilm-formation analysis from isolations from swine slaughterhouses as well as techniques to improve computational notebooks, rapid genotyping, and more.


Human gut microbiome

Time series strain tracking analysis post fecal transplantation identifies individual specific patterns of fecal dominant donor, recipient, and unrelated microbial strains – Koo & Morrow – PLOS One

Animal experiments

Microbiome complexity shapes metabolism – Litichevskiy & Thaiss – PLOS Biology

Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating – Heras & Martin – PLOS One

Molecular characterization and biofilm-formation analysis of Listeria monocytogenes, Salmonella spp., and Escherichia coli isolated from Brazilian swine slaughterhouses – Santos et al. – PLOS One

Animal microbiome

Ecology and distribution of Leptospira spp., reservoir hosts and environmental interaction in Sri Lanka, with identification of a new strain – Sluydts et al. – PLOS Neglected Tropical Diseases


A simple kit to use computational notebooks for more openness, reproducibility, and productivity in research – Figueiredo et al. – PLOS Computational Biology

Bacterial concentration and Campylobacter spp. quantification differ when fresh or ultra-frozen samples are analysed over time using molecular biology and culture-based methods – Khattak et al. – Plos One

Robust inference of population size histories from genomic sequencing data – Upadhya & Steinrücken – PLOS Computational Biology

Rapid genotyping of targeted viral samples using Illumina short-read sequencing data – Váradi et al. – PLOS One

Improved assessments of bulk milk microbiota composition via sample preparation and DNA extraction methods – Xue & Marco – PLOS ONE

September 8, 2022

Today’s microbiome digest includes ten rules for teaching yourself R, food safety profiles of ready-to-eat raw minced meat, teaching that addresses eco-anxiety, as well as both pathogenic and entomopathogenic fungi!

Human gut microbiome

Meals, Microbiota and Mental Health in Children and Adolescents (MMM-Study): A protocol for an observational longitudinal case-control study – Asbjornsdottir et al. – PLOS One

Strongyloides stercoralis infection induces gut dysbiosis in chronic kidney disease patients – Hai et al. – PLOS Neglected Tropical Diseases

Plant, root, and soil microbiome

Mycoparasitism capability and growth inhibition activity of Clonostachys rosea isolates against fungal pathogens of grapevine trunk diseases suggest potential for biocontrol – Geiger et al. – PLOS One

Impact of endophytic colonization by entomopathogenic fungi on the behavior and life history of the tobacco peach aphid Myzus persicae var. nicotianae – Wilberts et al. – PLOS One

Food microbiology

Bacteriological quality and antimicrobial susceptibility profiles of isolates of ready-to-eat raw minced meat from hotels and restaurants in Arba Minch, Ethiopia – Tonjo et al. – PLOS One


Ten simple rules for teaching yourself R – Lawlor et al. – PLOS Computational Biology


Laboratory evaluation of RealStar Yellow Fever Virus RT-PCR kit 1.0 for potential use in the global yellow fever laboratory network – Basile et al. – PLOS Neglected Tropical Diseases

Science, publishing, and career )

Insufficient yet improving involvement of the global south in top sustainability science publications – Dangles et al. – PLOS One

Adapt conservation biology teaching to address eco-anxiety in students – São Pedro et al. – PLOS Biology

August 19, 2022

Today’s digest is short and sweet! We have articles on the gut microbiota in rats, the influence of host genetics on the leaf fungal microbiome, and biofilm fouling in drinking water. There are also two manuscripts which provide tips for pathway enrichment analysis and biomarker discovery.

Happy reading! Also, if you’re in Scotland, consider checking out the enormous E. coli in Edinburgh this weekend!

Animal experiments

Effects of sea salt intake on metabolites, steroid hormones, and gut microbiota in rats – Chanmuang et al. – PLOS One

Plant, root, and soil microbiome

The changing influence of host genetics on the leaf fungal microbiome throughout plant development – Karasov & Lundberg – PLOS Biology

Water and extremophile microbiome

Impacts of temperature and hydraulic regime on discolouration and biofilm fouling in drinking water distribution systems – Fish et al. – PLOS Water


Nine quick tips for pathway enrichment analysis – Chicco & Agapito – PLOS Computational Biology

Ten quick tips for biomarker discovery and validation analyses using machine learning – Diaz-Uriarte et al. – PLOS Computational Biology