March 26, 2015

General microbiome

Note: not about metagenomics but 16S rRNA gene: The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies – J Paul Brooks – BMC Microbiology

Human respiratory microbiome

Spatial Variation in the Healthy Human Lung Microbiome and the Adapted Island Model of Lung Biogeography – Robert P. Dickson – Annals ATS

A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients – Mohammad A. Tariq – Frontiers in Microbiology

Perspective: Meningitis in adolescents: the role of commensal microbiota – James W.B. Moir – Trends in Microbiology

Human gut microbiome

An n=1 case report of a child with autism improving on antibiotics and a father’s quest to understand what it may mean – John Rodakis – Microbial Ecology of Health and Disease

Animal microbiome

Sponges: Culturable associated-bacteria of the sponge Theonella swinhoei show tolerance to high arsenic concentrations – Ray Keren – Frontiers in Microbiology

Soil, sediment, and plant root microbiome

Community Dynamics of Arbuscular Mycorrhizal Fungi in High-Input and Intensively Irrigated Rice Cultivation Systems – Yutao Wang – Applied and Environmental Microbiology

Analysis of methane-producing and metabolizing archaeal and bacterial communities in sediments of the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes – Zhichao Zhou – Frontiers in Microbiology

Isolation and characterization of culturable bacteria from bulk soil samples and the rhizosphere of arid-adapted Tylosema esculentum (Burchell). A. Schreiber (Marama bean) in Namibia – Olaf S. Kandjimi – African Journal of Biotechnology

Water microbiome

Prokaryotic assemblages and metagenomes in pelagic zones of the South China Sea – Ching-Hung Tseng – BMC Genomics

Spatial distribution of bacterial communities driven by multiple environmental factors in a beach wetland of the largest freshwater lake in China – Xia Ding- Frontiers in Microbiology

Review: Advancing the science of microbial symbiosis to support invasive species management: a case study on Phragmites in the Great Lakes – Kurt P. Kowalski – Frontiers in Microbiology

Extremophile microbiome (by special request!)

Colonization of plant substrates at hydrothermal vents and cold seeps in the northeast Atlantic and Mediterranean and occurrence of symbiont-related bacteria – Kamil M. Szafranski – Frontiers in Microbiology

Out of the dark: transitional subsurface-to-surface microbial diversity in a terrestrial serpentinizing seep (Manleluag, Pangasinan, the Philippines) – Kristin M. Woycheese – Frontiers in Microbiology

Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China – Brian P. Hedlund – Frontiers in Microbiology

Fatty Acids Profile of Microbial Populations in a Mining Reclaimed Region Contaminated with Metals: Relation with Ecological Characteristics and Soil Respiration – Ramya Narendrula and Kabwe K. Nkongolo – Journal of Bioremediation & Biodegradation

Bioreactor microbiome

Nitrogen addition, not initial phylogenetic diversity, increases litter decomposition by fungal communities – Anthony S. Amend – Frontiers in Microbiology

Food microbiology

Lipidomics as an important key for identification of beer-spoilage bacteria – Tomáš Řezanka – Letters in Applied Microbiology

Metabolomics

Combining reverse genetics and NMR-based metabolomics unravels trypanosome-specific metabolic pathways – Frédéric Bringaud – Molecular Microbiology

Resource niche overlap promotes stability of bacterial community metabolism in experimental microcosms – Ellard R. Hunting – Frontiers in Microbiology

Techniques

Enriching distinctive microbial communities from marine sediments via an electrochemical-sulfide-oxidizing process on carbon electrodes – Shiue-Lin Li and Kenneth H. Nealson – Frontiers in Microbiology

Microbes in presentations

Video: Dissertation seminar for Suzanne Ishaq – comparative analysis of the moose rumen microbiota

Video: Vance Kramer of Syngenta discussing microbiome research and comparing soil microbe communities across the US, Europe, and Brazil.

Video: Dr. Jan Jones from AgBiome discussing the plant-soil microbiome

Microbes in the news
Hidden Life Forms: Investigating Microbial Diversity on Our Bodies and in Our Homes – Jennifer Cutraro – New York Times

My uBiome Sleep Hacking Update – Alexandra Carmichael – uBiome

Antimicrobial CRISPR-Cas systems may be better weapons against bacteria than antibiotics – The Conversation

Secret Science Club Post-Lecture Recap: Big Data, Genomics and Urban Microbiomes – Big Bad Bald Bastard

Antibiotics For Gut Bacteria Show Surprising Results In Treating Autism – Dianne Depra – Tech Times

Harmless bacteria may be helpful against meningococcal outbreaks – Eureka Alert

New Software Offers Improved Read-based Identification in Metagenome Studies – Andrew P. Han – Genome Web

Not sure which one, but here it is: I am the bacterium that lives in your intestine – MicrobeWorld

FMTUpdate on OpenBiome – Paul Levy – Not Running A Hospital

Workshop on Planetary Protection Knowledge Gaps for Human Extraterrestrial Missions (Intro) – Jenna Lang – Microbe.net

Microbes and art

A Dress Made From Beer And Bacteria: C’est Chic Or C’est Ick? – Charles Poladian – IB Times

Science, publishing, and career

Developing microbiological learning materials for schools: best practice – James Redfern – FEMS Microbiology Letters

Sequencing and characterizing the genome of Estrella lausannensis as an undergraduate project: training students and biological insights – Claire Bertelli – Frontiers in Microbiology

Calling attention to poor speaker gender ratio – even when it hurts – Jonathan Eisen – The Tree of Life

The 5 Biases Pushing Women Out of STEM – Joan C. Williams – Harvard Business Review

Bik’s Picks

Jurrassic Park, but then with more furWoolly mammoth could be revived after scientists paste DNA into elephant’s genetic code – Andrew Griffin – The Independent

The Fitness Guru of the Trucking Industry – The Atlantic

On Friday, half a pair of twins leaves for a year in space. Here’s why that’s awesome. – Rachel Feltman – Washington Post

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March 25, 2015

General microbiome

Introducing npj Biofilms and Microbiomes – Staffan Normark – NPJ Biofilms and Microbiomes – includes:

Clinical Implications of Basic Science Discoveries: Immune Homeostasis and the Microbiome—Dietary and Therapeutic Modulation and Implications for Transplantation – J. A. Fishman, and A. W. Thomson – American Journal of Transplantation

Human respiratory microbiome

Clinical Outcomes and Microbiological Characteristics of Severe Pneumonia in Cancer Patients: A Prospective Cohort Study – Ligia S. C. F. Rabello – PLOS ONE

Human gut microbiome

Subsistence strategies in traditional societies distinguish gut microbiomes – Alexandra J. Obregon-Tito – Nature Communications

Variation in rural African gut microbiomes is strongly shaped by parasitism and diet – Elise R Morton – BioRxiv

Some problems with JAMA website, not sure if link works: Association of Gastrointestinal Symptoms With Autism – JAMA Psychiatry

Contrasting effects of fresh and fermented kimchi consumption on gut microbiota composition and gene expression related to metabolic syndrome in obese Korean women – Kyungsun Han – Molecular Nutrition & Food Research

Review: Alcohol and gut microbiota – the possible role of gut microbiota modulation in the treatment of alcoholic liver disease – G. Vassallo – Alimentary Pharmacology & Therapeutics

Perspective: Studies of the Gut: Overhyped or Underappreciated? – Tom Bartlett – The Chronicle of Higher Education

Review: Microbial biofilms and the human intestinal microbiome – Willem M de Vos – NPJ Biofilms and Microbiomes

Review: Microbial endocrinology: host-bacteria communication within the gut microbiome – Sara Sandrini – Journal of Endocrinology

Dead body microbiome

Potential Use of Bacterial Community Succession in Decaying Human Bone for Estimating Postmortem Interval – Franklin E. Damann – Journal of Forensic Sciences

Life after death: shallow-water Mediterranean invertebrate communities associated with mammal bones – Sergi Taboada – Marine Ecology

Animal microbiome

Monkeys: Adenovirus and Herpesvirus Diversity in Free-Ranging Great Apes in the Sangha Region of the Republic of Congo – Tracie A. Seimon – PLOS ONE

Birds (not birds and bees): Pathogenic bacteria and timing of laying – Anders Pape Møller – Ecology and Evolution

Arthropods: Metagenomic Profile of the Viral Communities in Rhipicephalus spp. Ticks from Yunnan, China – Han Xia – PLOS ONE

Nematodes: Associated bacteria of different life stages of Meloidogyne incognita using pyrosequencing-based analysis – Yi Cao – Journal of Basic Microbiology

Corals: Microbial Communities Associated with Healthy and White Syndrome-Affected Echinopora lamellosa in Aquaria and Experimental Treatment with the Antibiotic Ampicillin – David Smith – PLOS ONE

Water microbiome

Plant pathogenic bacteria in open irrigation systems: what risk for crop health? – J. R. Lamichhane and C. Bartoli – Plant Pathology

Elements of metacommunity structure and community-environment relationships in stream organisms – Jani Heino – Freshwater Biology

Opportunistic Pathogens and Elements of the Resistome that Are Common in Bottled Mineral Water Support the Need for Continuous Surveillance – Maria Fernanda Falcone-Dias – PLOS ONE

Bioinformatics

MUSiCC: a marker genes based framework for metagenomic normalization and accurate profiling of gene abundances in the microbiome – Ohad Manor and Elhanan Borenstein – Genome Biology

Average genome size estimation improves comparative metagenomics and sheds light on the functional ecology of the human microbiome – Stephen Nayfach and Katherine S Pollard – Genome Biology

Novel R Pipeline for Analyzing Biolog Phenotypic Microarray Data – Minna Vehkala – PLOS ONE

Ecology

Probabilistic Models to Describe the Dynamics of Migrating Microbial Communities – Joanna L Schroeder – PLOS ONE

Scanning the Landscape of Genome Architecture of Non-O1 and Non-O139 Vibrio cholerae by Whole Genome Mapping Reveals Extensive Population Genetic Diversity – Carol Chapman – PLOS ONE

Archaea

Phylogeny and Taxonomy of Archaea: A Comparison of the Whole-Genome-Based CVTree Approach with 16S rRNA Sequence Analysis – Guanghong Zuo – Life

Techniques

Open Labware: 3-D Printing Your Own Lab Equipment – Tom Baden – PLOS Biology

Microbes in the news

Treponema are not “ancient” but absence from some human’s guts is very interesting – Jonathan Eisen – Tree of Life
Refers to:
Ancient bacteria found in hunter-gatherer guts – Ann Gibbons – Science

With recipes: Upcoming book by Justin and Erica Sonnenburg: “The Good Gut” – Amazon

The ‘intraterrestrials’: New viruses discovered in ocean depths – National Science Foundation

Martian Microbes Could Be Eating Carbon Monoxide From the Atmosphere – Popular Mechanics

Kids with Autism Are More Likely to Have Gastrointestinal Problems – Rachael Rettner – LiveScience

Examining the connection between infant gut bacteria and food allergies – HealthCanal

Repeated Use of Antibiotics May Raise Diabetes Risk – Agata Blaszczak-Boxe – LiveScience

Bacteria in bud prompts B.C. medical marijuana firm to recall product – Canadian Press

Twin astronauts provide Earth-bound control for orbital health study – Phys.org

Baylor College of Medicine: Certain makeup can cause bacterial infections – John Brannen – Click2Houston

The Bathroom Blast Radius (And Your Toothbrush) – Dollar Shave Club

The Antibiotics Problem in Meat – Olga Khazan – The Atlantic

Satire: Biotechnology pressure group calls for an end to Saccharomyces abuse – The Allium

Science, publishing, and career

The 2015 Salary Survey Is Here – The Scientist

Women still find it tough to reach the top in science – Sharon Bell – The Conversation

Bik’s Picks

Ancient Egyptian skeleton shows signs of breast cancer – Elahe Izadi – Washington Post

Unravelling the hidden ancestry of American admixed populations – Francesco Montinaro – Nature Communications

Short Circuit in Magnets Postpone Re-Launch of CERN Collider – InsightTicker

Why the world needs biodiversity – Scientists have engineered the food that will help save a starving, warming planet – Jack Aldwinckle – Quartz

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March 24, 2015

General microbiome

Review: Biomarkers for the 21st Century: Listening to the Microbiome – Rodney Reynolds Dietert* and Ellen Kovner Silbergeld – Toxicological Sciences

Nice overview [PDF]: FASEB Releases “The Human Microbiome: Your Own Personal Ecosystem” – FASEB

Microbiome of pregnancy and birth

Bacterial diversity and Clostridia abundance decrease with increasing severity of necrotizing enterocolitis – Valarie E McMurtry – Microbiome

Human vaginal microbiome

Vaginal Microbiota of Adolescent Girls Prior to the Onset of Menarche Resemble Those of Reproductive-Age Women – Roxana J. Hickey – mBio

Human gut microbiome

The detection of vector-borne-disease-related DNA in human stool paves the way to large epidemiological studies – Alpha Kabinet Keita – European Journal of Epidemiology

Animal and other models

A Modified R-Type Bacteriocin Specifically Targeting Clostridium difficile Prevents Colonization of Mice without Affecting Gut Microbiota Diversity – Dana Gebhart – mBio

Effects of Irradiation on Epithelial Wound Healing and Microbial Diversity in an
in-vitro Oral Mucosa Model – Tine De Ryck – Nuclear Medicine & Radiation
Therapy

Microbes on Mars? Carbon monoxide as a metabolic energy source for extremely halophilic microbes: Implications for microbial activity in Mars regolith – Gary M. King – PNAS USA

Animal microbiome

Rabbit: The bacterial communities associated with fecal types and body weight of rex rabbits – Bo Zeng – Scientific Reports

Plant microbiome

Lots of omics words in this book: Microbiomics: An Approach to Community Microbiology – Pankaj Sharma – In: PlantOmics

Soil and plant root microbiome

The Soil Microbiome Influences Grapevine-Associated Microbiota – Iratxe Zarraonaindia – mBio

Elevated CO2 shifts the functional structure and metabolic potentials of soil microbial communities in a C4 agroecosystem – Jinbo Xiong – Scientific Reports

Isolation of a significant fraction of non-phototroph diversity from a desert Biological Soil Crust – Ulisses Nunes da Rocha – Frontiers in Microbiology

Nucleotide Sequences of Giant Viruses Found in Soil Samples of the Mojave Desert, the Prairie, the Tundra and the Antarctic Dry Valleys – Csaba Kerepesi – ArXiv

Impacts of bulk soil microbial community structure on rhizosphere microbiomes of Zea mays – Matthew G. Bakker – Plant and Soil

Pyrosequencing Reveals Changes in Soil Bacterial Communities after Conversion of Yungas Forests to Agriculture – Marcela S. Montecchia – PLOS ONE

Priority effects during fungal community establishment in beech wood – Jennifer Hiscox – The ISME Journal

Water microbiome

Very cool, with longitudinal plots: The Source of the River as a Nursery for Microbial Diversity – Luiz Felipe Valter de Oliveira, Rogério Margis – PLOS ONE

Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps – Adrien Vigneron – Applied and Environmental Microbiology

Site history and edaphic features override the influence of plant species on microbial communities in restored tidal freshwater wetlands – Christine E. Prasse – Applied and Environmental Microbiology

Multivariate and Phylogenetic Analyses Assessing the Response of Bacterial Mat Communities from an Ancient Oligotrophic Aquatic Ecosystem to Different Scenarios of Long-Term Environmental Disturbance – Silvia Pajares – PLOS ONE

Physiological and evolutionary potential of microorganisms from the Canterbury Basin subseafloor, a metagenomic approach – Frédéric Gaboyer – FEMS Microbiology Ecology

Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source – Eleftheria Antoniou – Frontiers in Microbiology

Built environment microbiome

Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments – Christine Moissl-Eichinger – Scientific Reports

Effect of Monochloramine Treatment on the Microbial Ecology of Legionella and Associated Bacterial Populations in a Hospital Hot Water System – Julianne L. Baron – Systematic and Applied Microbiology

Non-culturable bioaerosols in indoor settings: impact on health and molecular approaches for detection – Pascale Blais-Lecours – Atmospheric Environment

Probiotics

Development and Utility of the FDA “GutProbe” DNA Microarray for Identification, Genotyping, and Metagenomic Analysis of Commercially Available Probiotics – Jennifer N. Patro – Journal of Applied Microbiology

Antibiotics

Global trends in antimicrobial use in food animals – Thomas P. Van Boeckel – PNAS USA

Metagenomics and bioinformatics

Grid-Assembly: An oligonucleotide composition-based partitioning strategy to aid metagenomic sequence assembly – Tarini Shankar Ghosh – Journal of Bioinformatics and Computational Biology

Multiplexed metagenome mining using short DNA sequence tags facilitates targeted discovery of epoxyketone proteasome inhibitors – Jeremy G. Owen – PNAS USA

Insight into the evolution and origin of leprosy bacilli from the genome sequence of Mycobacterium lepromatosis – Pushpendra Singh – PNAS USA

Metabolomics / Ecology

Species interactions differ in their genetic robustness – Lon M. Chubiz – Frontiers in Microbiology

Microbes in the news

With a picture you will never forget: Acne and the Microbiome: Christi Investigates – Alexandra Carmichael

Vineyard’s soil microbes shape grapes’ microbial community – Science Daily

Crohn’s Disease: Gut Microbial Collaborations Trigger Inflammatory Diseases – PR NewsWire

Feed the microbes so they don’t feed on you: Fiber-Famished Gut Microbes Linked to Poor Health – Katherine Harmon Courage – Scientific American

Harnessing The Microbiome To Curb Hunger – Chemical and Engineering News

New Research On Appetite-Suppressing Bacteria Could Help Fight Obesity – Anna Almendrala

The MinION Access Programme (MAP) for portable DNA sequencing – Science Daily

Viruses, Archaea Self-mutate to Survive – Laboratory Equipment

Engineering and waterborne bacteria in building water systems – Colum Ó Bric -EngineersJournal

Gut and Mood: The Microbiome in Anxiety and Depression – Nina Weisbroth – Translational Microbiome

Not so fast, FastTree – Darling lab

SatireEach bioinformatician to have their own personal short-read aligner by 2016 – The Science Web

Not about Dr. WhoThe Doctor Who Tested Infectious Bacteria On Himself, And Won A Nobel – Ria Misra

Comparing the genomes of the leprosy bacteria – Phys.org

#BacteriaHysteria: “Beer Pong” Associated with Frequent Bacterial Transmissions – Jacquelyn Gray – HCPlive

#BacteriaHysteria: Clean machines: how to look after your tech and avoid bacteria bombs – David Howell – TechRadar

Science and art

Workshop, April 6, 2015: Paint with Bacteria – GenSpace, New York City’s Community Biolab

Science, publishing, and career

Opinion: Lay summaries needed to enhance science communication – Lauren M. Kuehne and Julian D. Olden – PNAS USA

50 min podcast: Women in Oceanography: Lots of Students, Not So Many Professors – Heather Goldstone – Capeandislands.com

This is why you shouldn’t believe that exciting new medical study – Julia Belluz – Vox.com

Stubborn Pay Gap Is Found in Nursing – Catherine Saint Louis – Catherine Saint Louis – New York Times

It’s time to reboot bioinformatics education – Todd Harris

Bik’s Picks

Macaques Exhibit a Naturally-Occurring Depression Similar to Humans – Fan Xu – Scientific Reports

Earth’s most powerful physics machine gets back in action – Marcus Woo – Wired.com

Scientists want museums to cut their ties with David Koch – Science Recorder

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March 23, 2015

General microbiome

Perspective: Separating the microbiome from the hyperbolome – Fergus Shanahan – Genome Medicine

Human oral microbiome

Interkingdom networking within the oral microbiome – Angela H. Nobbs, Howard F. Jenkinson – Microbes and Infection

Poster: Composition, Taxonomy And Functional Diversity Of The Oropharynx Microbiome In Individuals With Schizophrenia And Controls – Eduardo Castro Nallar – FigShare

Water microbiome

Targeted diversity generation by intraterrestrial archaea and archaeal viruses – Blair G. Paul – Nature Communications

Microbes in the News

Your poop could be a literal goldmine of precious metals – Rachel Feltman – Washington Post

Chlorine use in sewage treatment could promote antibiotic resistance – Science Daily

50 min podcast: Dr. Seth Bordenstein: Seeing Science and Symbiosis Through the Lens of an Evolutionary Microbiologist – People behind the science

New strategy to protect healthy gut microbes from antibiotics – Science Daily

Human parasites found in medieval cesspit reveal links between Middle East and Europe – Science Daily

Special Programmed Microbes May Help You Beat Obesity, Vanderbilt University Study – BioSpace

Skin microbiome may hold answers to protect threatened gold frogs from lethal fungus – Science Daily

Surviving in Hostile Territory: UCSB scientists discover a mechanism of self-mutation in mysterious viruses and microbes from within the Earth – Julie Cohen – UC Santa Barbara

Soaring antibiotic use in animals fuels ‘super bug’ fears – Chris Arsenault – Reuters

MRSA Is More Widespread Than You Think – Jason Tetro – Huffington Post Canada

Wolbachia Bacteria Can Control Mosquitoes with Fewer Chemicals – Erin Weeks – Entomology Today

Science, publishing and career

Social Media and Internet Driven Study Recruitment: Evaluating a New Model for Promoting Collaborator Engagement and Participation – Chetan Khatri – PLOS ONE

University of North Georgia apologized and agreed to stop distributing a course catalog with an denigrating picture – Travis Gettys – Raw Story

Bik’s Picks – Ancient rocks edition

When A Meteorite Hits Snow It Forms A “Snow Carrot” Instead Of A Crater – Mika McKinnon – Space io9

Telling stories from stone tools – Bruce Bower – Science News

Double Impact Crater in Canada Formed in Two Separate Impacts – Space Daily

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March 22, 2015

Human oral microbiome

Effect of dental cements on peri-implant microbial community: comparison of the microbial communities inhabiting the peri-implant tissue when using different luting cements – Michael Korsch – Clinical Oral Implants Research

Human vaginal microbiome

Fine-scale analysis of 16S rRNA sequences reveals a high level of taxonomic diversity among vaginal Atopobium spp. – Helena Mendes-Soares – Pathogens and Disease

Human gut microbiome

Fecal Microbiota in Patients with Irritable Bowel Syndrome Compared with Healthy Controls Using Real-Time Polymerase Chain Reaction: An Evidence of Dysbiosis – Ratnakar Shukla – Digestive Diseases and Sciences

Review: Psychobiotics and the gut–brain axis: in the pursuit of happiness – Zhou L – Neuropsychiatric Disease and Treatment

Microbiome of pregnancy and birth

The metabolomics of necrotising enterocolitis in preterm babies: an exploratory study – Amy Wilcock – The Journal of Maternal-Fetal & Neonatal Medicine

Colonisation of the gut by bifidobacteria is much more common in vaginal deliveries than Caesarean sections – Sarka Musilova – Acta Paediatrica

Review: The Birth of the Holobiont: Multi-species Birthing Through Mutual Scaffolding and Niche Construction – Lynn Chiu, Scott F. Gilbert – Biosemiotics

Animal models

Manipulation of the Quorum Sensing Signal AI-2 Affects the Antibiotic-Treated Gut Microbiota – Jessica Ann Thompson – Cell Reports

Vagotomy prevents the effect of probiotics on caspase activity in a model of postmyocardial infarction depression – M. Malick – Neurogastroenterology & Motility

Faecalibacterium prausnitzii prevents physiological damage in a chronic low-grade inflammation murine model – Rebeca Martín – BMC Microbiology

Peyer’s patch innate lymphoid cells regulate commensal bacteria expansion – Masaaki Hashiguchi – Immunology Letters

Animal microbiome

Review: A Review of Infectious Agents in Polar Bears (Ursus maritimus) and Their Long-Term Ecological Relevance – Anna C. Fagre – EcoHealth

Molecular characterization and diversity analysis of bacterial communities associated with Dialeurolonga malleswaramensis (Hemiptera: Aleyrodidae) adults using 16S rDNA amplicon pyrosequencing and FISH – Neeti Pandey andRaman Rajagopal – Insect Science

Genetic diversity of cyanophages of the myoviridae family as a constituent of the associated community of the Baikal sponge Lubomirskia baicalensis – T. V. Butina – Russian Journal of Genetics

Soil and plant root microbiome

Effects of Thinning and Induced Drought on Microbiological Soil Properties and Plant Species Diversity at Dry and Semiarid Locations – Javier Hedo de Santiago – Land Degradation & Development

Effect of Nitrogen Management on Soil Microbial Community and Enzymatic Activities in Jatropha curcas L. Plantation – Doongar Ram Chaudhary – CLEAN-Soil, Air, Water

Water microbiome

Quantifying the effects of geographical and environmental factors on distribution of stream bacterioplankton within nature reserves of Fujian, China – Yongming Wang – Environmental Science and Pollution Research

Spatial distribution of marine airborne bacterial communities – Jasmin S. Seifried – Microbiology Open

Microbiome of the built environment

Interphase microbial community characteristics in the fermentation cellar of Chinese Luzhou-flavor liquor determined by PLFA and DGGE profiles – Xiaofei Ding – Food Research International

Bioreactor microbiology

Microbial structures and community functions of anaerobic sludge in six full-scale wastewater treatment plants as revealed by 454 high-throughput pyrosequencing – Duntao Shu – Bioresource Technology

Cu and Cr enhanced the effect of various carbon nanotubes on microbial communities in an aquatic environment – Fei Wang – Journal of Hazardous Materials

Review: Utilization of microbial community potential for removal of chlorpyrifos: a review – Maya Yadav – Critical Reviews in Biotechnology

Metagenomics and Bioinformatics

A robust approach for identifying differentially abundant features in metagenomic samples – Michael B. Sohn – Bioinformatics

DomSign: a top-down annotation pipeline to enlarge enzyme space in the protein universe – Tianmin Wang – BMC Bioinformatics

The impact of quality filter for RNA-Seq – Pablo H.C.G. de Sáa – Gene

Poster: MetaGenSense: a web application for analysis and visualization of high throughput sequencing metagenomic data – Damien Correia – F1000 posters

SISPA-Seq for rapid whole genome surveys of bacterial isolates – Meredith S. Wright – Infection, Genetics and Evolution

Metabolomics

The metabolomics of necrotising enterocolitis in preterm babies: an exploratory study – Amy Wilcock – The Journal of Maternal-Fetal & Neonatal Medicine

Deep metabotyping of the murine gastro-intestinal tract for visualization of digestion and microbial metabolism – Silke S Heinzmann and Philippe Schmitt-Kopplin – Journal of Proteome Research

Application of a new Dynamic Gastrointestinal Simulator (SIMGI) to study the impact of red wine in colonic metabolism – C. Cueva – Food Research International

Isolation of dextran-hydrolyzing intestinal bacteria and characterization of their dextranolytic activities – Jin Kyoung Kim – Biopolymers

Diet-induced obesity causes metabolic impairment independent of alterations in gut barrier integrity – Caroline Kless – Molecular Nutrition & Food Research

Other microbial science papers

Detailed Contact Data and the Dissemination of Staphylococcus aureus in Hospitals – Thomas Obadia – PLOS Computational Biology

How bacterial pathogens colonize their hosts and invade deeper tissues – David Ribet, Pascale Cossart – Microbes and Infection

Antimicrobial stewardship: the role of scientists? – Laura Bowater – Journal of Antimicrobial Chemotherapy

Cyanobacteria: the bright and dark sides of a charming group – Katia Sciuto, Isabella Moro – Biodiversity and Conservation

Microbes in the news
The ChuckNorrisMicrobiome will get you, and you, and you … – Jonathan Eisen – The Tree of Life

The Oral Microbiota Affects More Than Just the Mouth – Lily Berrin – PLOS blog

This Week in Microbiology #100: Omnis cellula e cellula – MicrobeWorld

Wisconsin Longitudinal StudyDecades later, benchmark study yields data on human condition – Pamela Herd – Madison.com

Special microbes make anti-obesity molecule in the gut – American Chemical Society – Eureka Alert

About the baboon microbiome study: Are our gut bacteria swapped when we snuggle up? – Tim Sandle – Digital Journal

Wireless Sensors Help Scientists Map Staph Spread Inside Hospital – Scott Hensley – NPR

Science spreads antibiotic resistance by genetic experimentation – Karen Hardison – Digital Journal
Great #openaccess collection of papers on RNA – Jonathan Eisen – MicroBEnet

Visualizing the deadly threat of meningitis on campuses – Jamie Lampros – Standard Examiner

Epidemic Predicted by Bill Gates Who Pleads: Microbes Not Missiles – Jeanette Smith – Guardian LV

Science, publishing, and career

Forget Harvard and Stanford. It really doesn’t matter where you go to college – Jeffrey J. Selingo

Wolf Prize for Agricultural Science for Linda Saif: Award shines light on Ohio State scientist – Laura Arenschield – The Columbus Dispatch

Michigan judge asks PubPeer to turn over anonymous user information – Kelly Servick – Science Insider

In science, irreproducible research is a quiet crisis – Carolyn Johnson – Boston Globe

Bik’s Picks

Copy Cat: Someone Keeps Photocopying Their Cat at the University of Wisconsin Library – Samantha Grossman – Time.com

First fine-scale genetic map of the British Isles – Science Daily

Dude, why is my mushroom glowing? Scientists have the answer – Will Dunham – Reuters

March 20, 2015

General microbiome

Ribosomal RNA, the lens into life – Daniel McDonald, Zhenjiang Xu, Embriette R. Hyde and Rob Knight – RNA

Upcoming event: Cell Symposium: Human Immunity and the Microbiome in Health and Disease – September 27-29, 2015, Montreal, Canada

Human gut microbiome

Review: Firmicutes and Bacteroidetes involved in insulin resistance by mediating levels of glucagon-like peptide 1 – Claire Greenhill – Nature Reviews Endocrinology

Review: The impact of phytochemicals present in the diet on microbial signalling in the human gut – R.R. McCarthy – Journal of Functional Foods

Review: Targeting gut microbiota as a possible therapy for diabetes – Canxia He – Nutrition Research

Human oral microbiome

Guest editorial for special oral microbes edition – Robert Dwayne Lunsford – Microbes and Infection

Animal microbiome

Cattle: Microbial diversity analysis of subclinical mastitis in dairy cattle in Northeast China – Qingyu Jiang – African Journal of Microbiology Research

Worms: The effects of earthworms Eisenia spp. on microbial community are habitat dependent – Anna Koubová – European Journal of Soil Biology

Review: Insect Behaviour and the Microbiome – Zenobia Lewis, Anne Lizé – Current Opinion in Insect Science

Soil, sediments, and plant root microbiome

Carnivory does not change the rhizosphere bacterial community of the plant Drosera intermedia – Manuel Aira – Applied Soil Ecology

Microbial diversity and community respiration in freshwater sediments influenced by artificial light at night – Franz Hölker – Philosophical Transactions B
and
The biological impacts of artificial light at night: the research challenge – Kevin J. Gaston – Philosophical Transactions B

Microbial abundance and community in subsurface flow constructed wetland microcosms: role of plant presence – Qian Wang – Environmental Science and Pollution Research

Analysis of a population of magnetotactic bacteria of the Gulf of Gabès, Tunisia – Nathalie Pradel – Environmental Science and Pollution Research

Review: Linkages of plant-soil feedbacks and underlying invasion mechanisms – Inderjit and James F. Cahill Jr. – AoB Plants

Response of microbial communities to bioturbation by artificially introducing macrobenthos to mudflat sediments for in situ bioremediation in a typical semi-enclosed bay, southeast China – Ying Ma – Marine Pollution Bulletin

Water and snow microbiome

Microbial abundance and community structure in a melting alpine snowpack – Anna Lazzaro – Extremophiles

Diverse arsenic- and iron-cycling microbial communities in arsenic-contaminated aquifers used for drinking water in Bangladesh – Zahid Hassan – FEMS Microbiology Ecology

Metagenomics, proteomics, bioinformatics

Editorial: Microbial Diversity: A Bonanza of Phyla – Laura Eme, W. Ford Doolittle – Current Biology
belongs to study reconstructing Archaea genomes from metagenomic data: Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling – Cindy J. Castelle – Current Biology

Book Chapter: Evaluation of Combined Genome Assemblies: A Case Study with Fungal Genomes – Mostafa M. Abbas – Bioinformatics and Biomedical Engineering

Navigating through metaproteomics data – a logbook of database searching – Thilo Muth – Proteomics

Microbes in the news

Quorum-Sensing Molecule Modifies Gut Microbiota – Anna Azvolinsky – The Scientist

Functional Metagenomics Enables First Study of Bacterial Fitness in the Gut Microbiome – Chris Williams – Columbia Systems Biology

How to Study a Complex Microbial World- Part 1: DNA sequencing – Kevin Bonham -Scientific American

How to Study a Complex Microbial World – Part 2: Next Generation Sequencing Technology – Kevin Bonham -Scientific American

Biological oceanography: Life in the deepest depths
– Beth N. Orcutt – Nature Geoscience

1h Podcast/article: Say Cheese! Cheese history and science – GastroPod

Jailbreaking yeast could amp up wine’s health benefits, reduce morning-after headaches – Phys.org

29 min podcast: Queen Bee Microbiome (starts at 8 min), Yams, Resistence To Organic Checkoff – PRX Earth Eats

The World’s First €100M Microbiome-focused Venture Fund opened in France – Labiotech

Bioinformatics tool for metagenome analysis – Nancy Ambrosiano – R&D Magazine

Andreessen-Backed uBiome Is Now Doing An Indiegogo Campaign To Check Out Dental Bacteria – Kim-Mai Cutler – TechCrunch

Science, publishing, and career

“Canadian” Open-Access Journal Doesn’t Look Very Canadian – Jeffrey Beall – ScholarlyOA

Science and art

Art project: What Bacteria And Smell Have To Do With Feminism Today – Katherine Brooks – Huffington Post

Bik’s Picks

Morphological Mutations: Lessons from the Cockscomb Major comb variants and their genetic basis – Denis Headon – PLOS GeneticsWhat’s that thing?

How to Google Something You Don’t Know How to Describe – Adrienne Lafrance – The Atlantic

New Breast Milk Products Are on the Rise, but Commercialization Troubles Many – Andrew Pollack – New York Times

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March 19, 2015

Just a short digest today!

General microbiome

Review: The role of the microbiota in ageing: current state and perspectives – Denise B. Lynch – Wiley Interdisciplinary Reviews: Systems Biology and Medicine

Human gut microbiome / proteomics

Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota – Alessandro Tanca – Proteomics

Animal microbiome

AbaloneTemporal fluctuation in the abundance of alginate-degrading bacteria in the gut of abalone Haliotis gigantea over 1 year – Reiji Tanaka – Aquaculture Research

Soil microbiome

Native California soils are selective reservoirs for multidrug-resistant bacteria – Amanda C. Hollowell – Environmental Microbiology Reports

Techniques

Growing microbes in millifluidic droplets – Laurent Boitard – Engineering in Life Sciences

Other microbiology

Application of Whole-Genome Sequencing for Bacterial Strain Typing in Molecular Epidemiology – Stephen J. Salipante – Journal of Clinical Microbiology

Microbes in the news

Adventures in exploring the great indoors – Anne A. Madden – Fierer Lab

MO BIO Laboratories, Inc. presents the Second Annual MO BIO Microbiome Awards, offering more than $10,000 in prizes! – MoBio

uBiome — Explore Your Dental Microbiome – IndieGoGo
uBiome Launches World’s First Dental Citizen Science Campaign – Global Newswire

Humans Need ‘Stronger’ Breast-Milk Than Most Mammals – Brian Stallard – Nature World News

Interactive/videos: The good, the bad and the ugly in everyday bacteria – CBC news

Los Alamos scientists develop new method for metagenomic analysis – News Medical Net

This week’s  Google Plus Microbiology Journal club: Cheese Microbe Communities – GPlusMicroJC

Researchers have a gut feeling – could chronic pain be caused by bacteria? – Michael Brooks – New Statesman

MeMed’s blood test accurately distinguishes bacterial and viral infections, research shows – Medical Xpress

Turning the gut microbiome into a chat room – Tina Hesman Saey – ScienceNews

Science, publishing, and career

A grad school survival guide – Andrew Gaudet – Science

Bik’s Picks

The New York Times’ smartwatch cancer article is bad, and they should feel bad – Russell Brandom – The Verge

Unmasked: – What 10 million passwords reveal – about the people who choose them – WP engine

All other languages tired of Python’s sh!t – The Science Web
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March 18, 2015

Human skin microbiome

Book (hopefully also covering women’s skin): The Epidermis of Man: Co-existing with Commensals – Eds. Peter J Heidt et al.

Human oral microbiome

Salivary Microbiota Associated with Immunoglobulin A Nephropathy – Maria Piccolo – Microbial Ecology

Human gut microbiome

ReviewGut bacteria in children with autism spectrum disorders: challenges and promise of studying how a complex community influences a complex disease – Rosa Krajmalnik-Brown – Microbial Ecology in Health and Disease

Microbiome of pregnancy and birth

The Perinatal Microbiome and Pregnancy: Moving Beyond the Vaginal Microbiome –
Prince AL – CSH Perspect Med (only NCBI link available as of now)

Association between breastfeeding and intelligence, educational attainment, and income at 30 years of age: a prospective birth cohort study from Brazil – Cesar G Victora – The Lancet

Review: Individualized medicine and the microbiome of the reproductive tract – Andrea G. Braundmeier – Frontiers in Physiology

Animal and other models

The Quorum Sensing Peptides PhrG, CSP and EDF Promote Angiogenesis and Invasion of Breast Cancer Cells In Vitro – Bart De Spiegeleer – PLOS ONE

Registered report of a replication study: Intestinal inflammation targets cancer-inducing activity of the microbiota – Kate Eaton – eLIFE

Ablation of Tumor Necrosis Factor Is Associated with Decreased Inflammation and Alterations of the Microbiota in a Mouse Model of Inflammatory Bowel Disease – Yava L. Jones-Hall – PLOS ONE

Phytase Modulates Ileal Microbiota and Enhances Growth Performance of the Broiler Chickens – Anna Ptak – PLOS ONE

Intestinal Microbiota Succession and Immunomodulatory Consequences after Introduction of Lactobacillus reuteri I5007 in Neonatal Piglets – Chengli Hou – PLOS ONE

Animal microbiome

MonkeysMetagenomic analysis of the Rhinopithecus bieti fecal microbiome reveals a broad diversity of bacterial and glycoside hydrolase profiles related to lignocellulose degradation – Bo Xu – BMC Genomics

FrogsComposition of symbiotic bacteria predicts survival in Panamanian golden frogs infected with a lethal fungus – Matthew H. Becker – Proceedings of the Royal Society B

CowsAltered Microbiomes in Bovine Digital Dermatitis Lesions, and the Gut as a Pathogen Reservoir – Martin Zinicola – PLOS ONE

Culture based, horses: Occurrence of bacteria and polymorphonuclear leucocytes (PMNLs) in foetal compartments at parturition; relationships with foal and mare health in the peripartum period- E. Hemberg – Theriogenology

BeesVariation in Honey Bee Gut Microbial Diversity Affected by Ontogenetic Stage, Age and Geographic Location – Zuzana Hroncova – PLOS ONE

Bees: Deep Sequencing and Ecological Characterization of Gut Microbial Communities of Diverse Bumble Bee Species – Haw Chuan Lim – PLOS ONE

Sponges: Molecular investigation of the microbial community associated with the fire sponge, Tedania ignis, in Bermuda – Nicholas J Jouett – PeerJ

Millipedes: Microbial dynamics in food, intestine and fecal pellets of two endemic pill-millipedes (Arthrosphaera: Sphaerotheriida) of the Western Ghats [PDF] – C.N. Ambarish and K.R. Sridhar – Journal of Agricultural Technology

Mosquitoes: RNA shotgun metagenomic sequencing of northern California (USA) mosquitoes uncovers viruses, bacteria, and fungi – James Angus Chandler – Frontiers in Microbiology

Soil and plant root microbiome

Impact of metagenomic DNA extraction procedures on the identifiable endophytic bacterial diversity in Sorghum bicolor (L. Moench) – Mapula Kgomotso Annah Maropola – Journal of Microbiological Methods

Rhizosphere microbial community manipulated by 2 years of consecutive biofertilizer application associated with banana Fusarium wilt disease suppression – Zongzhuan Shen – Biology and Fertility of Soils

The Effect of Phylogenetically Different Bacteria on the Fitness of Pseudomonas fluorescens in Sand Microcosms – Olaf Tyc – PLOS ONE

Organic amendments in avocado crops induce suppressiveness and influence the composition and activity of soil microbial communities – Nuria Bonilla – Applied and Environmental Microbiology

Water microbiome

The biogeography of abundant and rare bacterioplankton in the lakes and reservoirs of China – Lemian Liu – The ISME Journal

Marine Fungi from Deep Subseafloor Sediments: Species richness and Adaptation -Vanessa Rédou – Applied and Environmental Microbiology

Microbial Methane Potential for the South Sumatra Basin Coal: Formation Water Screening and Coal Substrate Bioavailability – Rita Susilawati – Energy Procedia

Site history and edaphic features override the influence of plant species on microbial communities in restored tidal freshwater wetlands – Christine E. Prasse – Applied and Environmental Microbiology

Spatial variability of microbial assemblages associated with a dominant habitat-forming seaweed – Alexandra H. Campbell – Frontiers in Microbiology

Bioreactor microbiome

Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing – Jianhua Guo – Microbial Cell Factories

Anaerobic codigestion of pretreated wheat straw with cattle manure and analysis of the microbial community – Zilin Song – Bioresource Technology

Analysis of Microbial Communities in the Oil Reservoir Subjected to CO2-Flooding by Using Functional Genes as Molecular Biomarkers for Microbial CO2 Sequestration – Jin-Feng Liu – Frontiers in Microbiology

Bacterial and archaeal guilds associated with electrogenesis and methanogenesis in paddy field soil – Bo Lin – Geoderma

Food microbiology

Comparing natural and selected starter cultures in meat and cheese fermentations – Daniela Bassi – Current Opinion in Food Science

Phages and viruses

PerspectiveThe Next Epidemic — Lessons from Ebola – Bill Gates – The New England Journal of Medicine

Genomic characteristics and environmental distributions of the uncultivated Far-T4 phages – Simon Roux – Frontiers in Microbiology

Isolation of Phages for Phage Therapy: A Comparison of Spot Tests and Efficiency of Plating Analyses for Determination of Host Range and Efficacy – Mohammadali Khan Mirzaei – PLOS ONE

Evaluation of a Viral Microarray Based on Simultaneous Extraction and Amplification of Viral Nucleotide Acid for Detecting Human Herpesviruses and Enteroviruses – Yi Liu – PLOS ONE

Review: Origins and evolution of viruses of eukaryotes: The ultimate modularity – Eugene V. Koonin – Virology

Metagenomics and bioinformatics

Accurate read-based metagenome characterization using a hierarchical suite of unique signatures – Tracey Allen K. Freitas – Nucleic Acid Research

k-mer analysisCrossing the streams: a framework for streaming analysis of short DNA sequencing reads – Qingpeng Zhang – PeerJ

Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum – Keith H. Turner – PNAS USA

Hypothesis: Systems modeling approaches for microbial community studies: From metagenomics to inference of the community structure – Mark Hanemaaijer – Frontiers in Microbiology

Metabolomics

Review: Role of Resistant Starch in Improving Gut Health, Adiposity, and Insulin Resistance – Michael J Keenan – Advances in Nutrition

Microbial Degradation of Whole-Grain Complex Carbohydrates and Impact on Short-Chain Fatty Acids and Health – Knud Erik Bach Knudsen – Advances in Nutrition

Microbes in the news

Pet Peeves on 16S rRNA gene sequencing – Noah Fierer – Fierer lab

Can Probiotic Bacteria Save An Endangered Frog? – Ed Yong – National Geographic

New paper examines social effects on the gut microbiome of wild baboons – William G. Gilroy – Notre Dame News

Breast-Feeding Boosts Chances Of Success, Study In Brazil Finds – Rob Stein – NPR

Breast-Feeding May Have Benefits Decades Later – Nicholas Bakalar – New York Times

Lab creates bioinformatics tool for metagenome analysis – Phys.org

Could Man’s Best Friend Be Man’s Best Medicine? – Alexis Blue – University of Arizona

Dog germs may be good for you – Bradley J. Fikes – UT San Diego

The vaginal microbiome changes during and after pregnancy – American Microbiome Institute

Can the bacteria in your bowels increase your cancer risk? – Amy Yurkanin – AL.com

Your microbiome: groundbreaking research about our gut bacteria shows it could be the key to health – Jacqui Gibbons – High 50 Health

From human gut to the smartphone, new health monitoring – Tim Sandle – Digital Journal

Parasite turns shrimp into voracious cannibals – Chris Bunting – University of Leeds

Gut Bacteria, ASD, and Probiotics: Exploring the Relationship – Elizabeth Gough-Gordon – EMPR

New Molecular Tool Assesses Vaginal Microbiome Health, Diagnosis Infections-Fast – ASM

Technology helps unlock secrets of rare bacteria – Phys.org

Stanford researchers unravel secrets of shape-shifting bacteria – Tom Abate and Chris Cesare – Stanford News

VideoAlexander Fleming – Inventor of Penicillin – Scientiflix

Bik’s Picks

Ultrasoundings: monitoring a pregnant dolphin – Bill Van Bonn – Shedd Aquarium

Why are there so many freaking beetles? Science may finally have the answer. – Rachel Feltman – Washington Post

The myopia boom: Short-sightedness is reaching epidemic proportions. Some scientists think they have found a reason why – Elie Dolgin – Nature

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March 17, 2015

Happy St. Patrick’s day! Enjoy the green microbial fermented fluids.

Human oral microbiome

Metagenomic sequencing reveals altered metabolic pathways in the oral microbiota of sailors during a long sea voyage – Weiwei Zheng – Scientific Reports

A Filifactor alocis-centered co-occurrence group associates with periodontitis across different oral habitats – Hui Chen – Scientific Reports

Review: Polymicrobial nature of chronic oral disease – Stuart Dashper – Microbiology Australia

Animal models

Intestinal Microbiota Composition Modulates Choline Bioavailability from Diet and Accumulation of the Proatherogenic Metabolite Trimethylamine-N-Oxide – Kymberleigh A. Romano – mBio

Animal microbiome

Social networks predict gut microbiome composition in wild baboons – Jenny Tung – eLIFE

Unfolding the secrets of coral–algal symbiosis – Nedeljka Rosic – The ISME Journal

Water microbiome

Presence of oxygen and aerobic communities from sea floor to basement in deep-sea sediments – Steven D’Hondt – Nature Geoscience

Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea – Mohd Ikram Ansari – Scientific Reports

Oceanographic structure drives the assembly processes of microbial eukaryotic communities – Adam Monier – The ISME Journal

Other microbial science

Microbial reflectance spectra: Surface biosignatures of exo-Earths: Remote detection of extraterrestrial life – Siddharth Hegde – PNAS USA

Viruses and phages

Membrane vesicles in natural environments: a major challenge in viral ecology – Nicolas Soler – The ISME Journal

Lytic phages obscure the cost of antibiotic resistance in Escherichia coli – Samuel J Tazzyman and Alex R Hall – The ISME Journal

Metagenomics

Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy – Masaki Shintani – Frontiers in Microbiology

Bioinformatics

Minimum entropy decomposition: Unsupervised oligotyping for sensitive partitioning of high-throughput marker gene sequences – A Murat Eren – The ISME Journal

Microbes in the news

Baboon friends swap gut germs – Science Daily

No limit to life in deep sediment of ocean’s “deadest” region – National Science Foundation

The microbiome of alcoholics may contribute to pathologies – American Microbiome Institute

Reflectance spectra from microbes: This Alien Color Catalog May Help Us Spot Life on Other Planets – Jesse Emspak – Smithsonian

New Data May Help Identify Extraterrestrial Life – William Large – Clapway

Science, publishing, and career

Competitive Science: Is Competition Ruining Science? – Ferric C. Fang and Arturo Casadevall – Infection and Immunity

Bik’s Picks – Marine Mammal Edition

Starving sea lion pups inundate Southern California rescue centers – Marty Graham – Reuters

Sciencespeak: Whale Pump. Whales can poop almost anywhere they want. – Brian Switek – National Geographic

Long-lost whale fossil sheds light on East Africa’s evolution – Bianca Nogrady – ABC Science

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The Vaginal Microbiome

An overview of large studies on the human vaginal microbiome. Please leave a comment if I missed an important paper, which is extremely likely. This is just a first draft, and this post is meant to be continuously updated.

Scientific papers, most recent first

The vaginal microbiome during pregnancy and the postpartum period in a European population – David A. MacIntyre – Scientific Reports – March 2015

  • Subjects: 42 British women with term delivery, sampled longitudinally, 3 ethnic groups
  • DNA extraction: bead-beating + QIAamp DNA Mini kit
  • Sequencing: V1-V2 region, MiSeq, 2,113,251 reads, 14088 reads/sample (rarefied to 1916?)

The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women – Roberto Romero – Microbiome – February 2014

  • Subjects: 32 non-pregnant women, 22 pregnant women who delivered on time
  • DNA extraction: Bead-beating, ZR Fecal DNA extraction kit (ZYMO Research)
  • Sequencing: V1-V2 region, 454 FLX Titanium, 2,946,507 reads, 2878 reads/sample

Diversity of the Vaginal Microbiome Correlates With Preterm Birth – Richard W. Hyman – Reproductive Sciences – January 2014

  • Subjects: 88 pregnant women (various ethnicities), 143 swabs
  • DNA extraction: QIAgen DNeasy Tissue and Blood Kit
  • Sequencing: 8F-1492R, clone library, Sanger sequencing, read from one end? not clear.
    Number of clones not clear?

Diverse Vaginal Microbiomes in Reproductive-Age Women with Vulvovaginal Candidiasis
Mu-Biao Liu – PLOS ONE – November 2013

  • Subjects: 95 women, some with BV or VVC, 226 swabs
  • DNA extraction: DNA Magnetic Sand Extract kit (BioEAsy,China)
  • Sequencing: V6 region, Illumina HiSeq 2000, 1,026,382 reads, 4,542 reads/sample

A Metagenomic Approach to Characterization of the Vaginal Microbiome Signature in Pregnancy – Kjersti Aagaard – PLOS ONE – June 2012

  • Subjects: 24 pregnant women, 60 non-pregnant controls, multiple timepoints/subject
  • DNA extraction: PowerSoil DNA Isolation Kit
  • Sequencing: V3-V5 region, 454 FLX Titanium, 2.5 million reads, 6837 reads/sample

$$ Temporal Dynamics of the Human Vaginal Microbiota – Pawel Gajer – Science Translational Medicine – May 2012

  • Subjects: 32 women, 937 samples
  • DNA extraction: Bead-beating with ZR Fecal DNA extraction kit (Zymo Research)
  • Sequencing: V1-V2 region, 454 Titanium, 2,522,080 reads, 2692 sequences/sample

Vaginal Microbiome and Epithelial Gene Array in Post-Menopausal Women with Moderate to Severe Dryness – Ruben Hummelen – PLOS ONE – November 2011

  • Subjects: 32 post-menopausal women, some sampled over 10 weeks
  • DNA extraction: Instagene (Bio-Rad)
  • Sequencing: V6 region, Illumina, 1,758,430, 17,410 reads/sample

Vaginal microbiome of reproductive-age women – Jacques Ravel – PNAS USA – March 2011

  • Subjects: 396 North American women representing four ethnic groups
  • DNA extraction: Bead beating combined with QIAamp DNA mini kit
  • Sequencing: V1-V2 region, 454 FLX, 897,345 good-quality reads, 2200 reads/sample

Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women – Xia Zhou – The ISME Journal – May 2007

  • Subjects: 144 women, 2 ethnicities
  • DNA extraction: not listed
  • Sequencing: T-RFLP, clone library , 6000 clones, 90 clones/sample

Microbes on the human vaginal epithelium – Richard W. Hyman – PNAS USA – April 2005

  • Subjects: 20 women
  • DNA extraction: QIAgen DNAeasy
  • Sequencing: 8F x 1492R, ~1000-2000 clones per subject

Review papers

The vaginal microbiome: rethinking health and diseases – Bing Ma – Annual Reviews in Microbiology – September 2013

Understanding vaginal microbiome complexity from an ecological perspective – Roxana J. Hickey – Translational Research – October 2012

A New Era of the Vaginal Microbiome: Advances Using Next-Generation Sequencing – Jennifer M. Fettweis – Chemistry & Biodiversity – May 2012

The vaginal microbiome in health and disease – Bryan A. White – Trends in Endocrinology & Metabolism – October 2011

The vaginal microbiome: new information about genital tract flora using molecular based techniques – RF Lamont – BJOG: An International Journal of Obstetrics & Gynaecology
– April 2011

News articles

Sweet Peach won’t make vaginas smell like fruit or taste of Diet Coke – Hannah Jane Parkinson – The Guardian – November 2014

What’s in Your Vagina? A healthy microbiome, hopefully. Moises Velasquez-Manoff – Slate – January 2013

Characterizing the “Healthy” Vagina – Jef Akst – The Scientist – May 2014

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