Hi all,

I have decided to take (at least) a year off from paid work to focus on my research integrity work. Since 2013, I have worked on finding plagiarism and image duplication in scientific papers. Every free minute I searched for papers, made reports highlighting the potential problems, and wrote to journals and institutions about these concerns.

Together with Arturo Casadevall, Ferric Feng and other co-authors, we published three papers about our work on the frequency of image duplication in biomedical papers. You can find them here:

• The Prevalence of Inappropriate Image Duplication in Biomedical Research Publications – Elisabeth M. Bik, Arturo Casadevall, Ferric C. Fang – mBio. 2016 Jun 7;7(3). pii: e00809-16. doi: 10.1128/mBio.00809-16
• Testing Hypotheses on Risk Factors for Scientific Misconduct via Matched-Control Analysis of Papers Containing Problematic Image Duplications – Daniele Fanelli, Rodrigo Costas, Ferric C. Fang, Arturo Casadevall, Elisabeth M. Bik – Sci Eng Ethics. 2018 Feb 19. doi: 10.1007/s11948-018-0023-7
• Analysis and Correction of Inappropriate Image Duplication: the Molecular and Cellular Biology Experience – Elisabeth M. Bik, Ferric C. Fang, Amy L. Kullas, Roger J. Davis, Arturo Casadevall – Mol Cell Biol. 2018 Sep 28;38(20). pii: e00309-18. doi: 10.1128/MCB.00309-18

As of today, I have reported 85 papers and theses for extensive plagiarism, and over 1200 papers for potential image duplication. But I have many more that I still need to report, and I am getting more and more requests for help, and the work – all done in my spare time – was piling up.

So after working for 2.5 years in microbiome startup companies, I have decided to take at least a year off – maybe longer – so that I will have much more time to work on this project. I will still closely follow the microbiome field, of course, as well.

Thank you all, for your support!

Elisabeth Bik

Here is the unroll of a Twitter thread on this topic, as done by ThreadReader.

I am taking a year off from paid work to focus more on my science misconduct volunteer work. Science needs more help to detect image duplication, plagiarism, fabricated results, and predatory publishers.

Most of the work detecting these problems in science papers is done by volunteers like me. It takes perseverance and patience. Many journals, authors, and academic institutions will not take action.

Even if they respond, It might take years before papers with serious flaws are corrected. All that time, those papers are not flagged by the journals, and others researchers might cite them or base their research on them.

As of now, we can only flag papers on @PubPeer and install their plugin so you can see which papers have a comment, e.g. when doing literature searches.

And I will still write to journals or institutions about all papers with concerns that I found so far. Even if it takes hours to find their contact info.

I still have 100s of papers that I need to officially report and 100s of reported ones to follow up on. The only way I felt I can catch up on that is to quit my paid job. Which is scary.

It would be nice if journals, institutions, funding agencies, and countries would care more about the quality of their research. If they had more guts to respond to concerns raised by readers – and take action.

The work that volunteers like us do is not very rewarding obviously. No one likes criticism. It can also be dangerous. Authors might start personal attacks on us and sue us for libel.

I am also very aware of the collateral damage, e.g. of coauthors who did not commit misconduct, others workers in those labs, and the effects on family members.

With that in mind, it is important to focus on facts, on the potential problems in the papers and how to address those. The focus should be on the papers, not the authors.

I might make an exception in cases of authors using false affiliations and fake coauthors.

The more you dig into these cases the more other weird stuff you find. I can probably do this full-time for the rest of my life. Maybe I will.

An open peer review of a preprint paper by Hatch A et al. from Viome, posted on OSF Preprints, January 2019.

Full disclosure: I worked for Viome’s competitor uBiome from October 2016 – December 2018. I am currently an independent consultant.

A robust metatranscriptomic technology for population-scale studies of diet, gut microbiome, and human health

Andrew Hatch, James Horne, Ryan Toma, Brittany L. Twibell, Kalie M. Somerville, Benjamin Pelle, Kinga P. Canfield, Guruduth Banavar, Ally Perlina, Helen Messier, Niels Klitgord and Momchilo Vuyisich*

Viome, Inc, Los Alamos, NM 87544, United States.

Background information

In a recent paper authored by Stanford researchers, biotech startups were criticized for not sharing their discoveries through peer-reviewed research studies. So in that light, it is great to see a biotech company such as Viome publish a study about their microbiome consumer product. Viome’s leaders have been very vocal about the superiority of their product – which is based on RNA transcription – over that of other microbiome consumer tests, which are based on DNA amplification and sequencing. But until this preprint came out, no research on the Viome product had been published. So I was excited to hear about this preprint!

It is important to first point out that this paper is not a peer-reviewed paper. It is a preprint, which means it is written as an academic paper, but it has not been peer-reviewed by other scientists. It is a first step, however, to share the work that Viome did to build a metatranscriptomics platform, and show some of their first results. I hope my comments will be useful in the process of getting this study published in a peer-reviewed journal.

The paper describes Viomega, Viome’s automated stool metatranscriptomics method that involves RNA extraction from stool samples, sequencing, and bioinformatics analysis. Let’s go over each of the sections.

Introduction

The introduction of the paper is mostly stating how metatranscriptomics is superior to other techniques. It has a somewhat oversimplified table showing other methods (bad!) to their own method (good!).

For example, under “Method Biases”, the 16S Gene Sequencing column states “Heavily influenced by amplification method, but also sequencing quality, sample lysis, and bioinformatics“, where “heavily influenced” sounds a bit denigrating, while sequencing quality and bioinformatics are conveniently left out in the Metatranscriptomics column.

The “identifies all living organisms” appears oversimplified as well. First, is a phage or virus alive? Then, how about a bacterium that is in a viable-non-culturable or spore-state? It is alive, but it is probably not transcribing much RNA – can metatranscriptomics detect those?

The statement that Metatranscriptomics “allows assessment of pathway activities that can lead to personalized health insights and recommendations with molecular-level precision” is overly subjective and is not proven in this paper. That last part of the sentence sounds like a Viome commercial, not like something that belongs in a scientific paper.

The introduction text states that 16S gene sequencing misses archaea or eukaryotes, but fails to acknowledge that most of these can be identified with broad-range primers as well.

In short, the Introduction shows a too black- and white comparison between different microbial community methods that is not very objective.

Methods

Not unexpectedly from a biotech company, the paper does not provide a lot of technical details on sample extraction, library preparation, or bioinformatics analysis. In order to pass peer review, the authors will likely need to provide more details on their methods – so that others can easily replicated them. The statements about participant consent and IRB approval were also very short; most journals and peer reviewers would like to see something more than “all study procedures were approved by an IRB” from a non-academic institution. 

Results: Validation and reproducibility

The first part of the Results shows that the Viomega method can detect a range of different microorganisms with relative equal efficiency and precision. Using mock communities, the results are accurate, and reported to contain no false positives or negatives or sample-to-sample crosstalk. However, data given here were very sparse.

Figure 4 is a colorful representation of the mock community sequencing experiment, but is lacking percentages and other details, such as number of reads. Where there really no false positive or false negative reads? Not a single one? There is also no word on how the negative controls did perform over their tests of 10,000 samples. It would have been really valuable to have compared this mock community using all three methods compared in Table 1; 16S, metagenomics, and metatranscriptomics.

This section of the Results also shows that the Viome test is reproducible. Three different experiments were performed in which small numbers of participants (3 to 7 persons) collected stool samples testing the following:

1. stool collection from different part of their stool sample (Figure 5)
2. waiting 0, 3, or 10 min before adding stool to the kit tube (Figure 6)
3. collecting stool on 3 different days over a period of two week (Figure 7)

In all three experiments, samples from the same stool specimen or individual were very similar to each other, showing that the Viome test results are reproducible (the dark squares along the diagonal in Figures 5, 6, 7).

Is Gene Function Composition better than simple Taxonomy?

Figures 5/6/7 show the test results in two different ways. The A panels in each figure are based on which active microbes are present in the samples, so based on the taxonomy of the RNA reads. The B panels are the results based on gene function composition (which genes are being expressed in that sample).

It was interesting to see that in all cases the microbial composition (taxonomy; A panels) was better able to tell individuals apart than the gene expression (B panels).

Detailed explanation: If you look at the A panels, the comparison of each person to other samples from the same person shows they are very similar (purple) while each individual is very different (light blue) from other persons. In the B panels, the individuals do not differ that much from each other; all individual-to-individual comparisons are darker shades of purple that are more difficult to tell apart. 

That is a bit ironical, because the preprint states at the beginning (Abstract and Table 1) that functional gene analysis, not microbial composition based on taxonomy, is needed for personalized health insights. Instead, the paper appears to show that each person has their own, personal gut microbiome, while the functional capacities appear to be pretty similar between individuals.

The paper does not provide an answer how these small person-to-person variations in microbial gene expression will lead to the “goal to develop personalized nutrition algorithms” as stated in the Abstract.

Transcriptomics taxonomy prevalence data from 10,000 samples

Metatranscriptomics is superior to other techniques, stated the paper in the Introduction and Table 1, but the paper does not do anything to prove that. There was no comparison of 16S sequencing vs metatranscriptomics on e.g., the mock community shown in Figure 4. Such a comparison could add a lot of value to the paper, and could support the bold statements made in the Introduction.

Also, the amount of viruses, archaea, and eukaryotes in the sample set was not very high, suggesting that 16S sequencing does not miss as much diversity as the authors claim in the introduction. For example, strain level analysis (Table S1) shows crAssphage as the most prevalent virus, but it is ranked #144 of all taxa (26%). Similarly, Methanobrevibacter clocks at #269 (14%), while Entamoeba is the most prevalent eukaryote at #336 (10% of samples).

Many of the viruses/phages found in the stool samples appear to be plant associated (Suppl tables). For example, Phaseolus vulgaris endornavirus (beans), Pepper mild mottle virus, Cannabis cryptic virus, and shallot latent virus are among the most prevalent viruses. Could the authors comment on this? Could these be transient microbiome components that were part of a food item, or are they present in the same person over longer periods of time? It would be nice to see some data on that.

Several taxa appear to behave very differently on strain/species/genus level (Tables S1-S3). Examples where genus-level prevalence is much lower than expected based on strain prevalence are given below. In other examples, taxa with high prevalence at genus level seem to disappear at species or strain level; also given below. 

Unfortunately, relative abundance data appears to be missing. How abundant are viruses, archaea and eukaryotes ? Which percentage of the transcriptomics reads were assigned to each of these groups? These numbers, now missing, would tell us which groups 16S sequencing would miss and would allow for a better comparison of different microbial community analysis tools.

Can Viome’s microbiome analysis connect the diet and the gut metatranscriptome?

The paper contained very few functional results on the data from 10,000 Viome samples. Viome has repeatedly claimed that transcriptomics are much more informative than just lists of microbial taxa, and the abstract promises “several small clinical studies to demonstrate the connections between diet and the gut metatranscriptome.”

Therefore, it was disappointing to see that the paper was mainly limited to taxonomic assignments. This dataset, which is one of the largest of its kind, sounded very promising, and I had hoped to see many more functional analyses on these samples.

Table 4 is the only part of the study that analyzes the functional capacity of the data from 10,000 samples. It lists the top 12 KEGG functions (although the legend says “top 10”; see below). Unfortunately, this table is not very informative and might contain some errors (see below). Most importantly, it is just a list of genes without any discussion on their function.

In addition, if the prevalence of these genes is over 99.9% (meaning that almost every person’s microbiome contains those genes), how can one use that for the correlation of microbial taxa or genes with lifestyle or diet? Based on the top 10 or top 100 (Table S4) KEGG functions, all subjects’ microbiomes appear to have the exact same genes. How is this functional data superior to the big inter-individual differences that are found by  the more conventional 16S types of microbiome analyses?  The paper would be much stronger if this important point would be discussed.

Summary of findings

In summary, the strengths of this paper are the experiments showing that the Viomega technique is reproducible. That is a great paper by itself, but the current title and abstract are promising much more than the paper currently delivers. With a transcriptomics dataset of 10,000 stool samples, and repeated claims that transcriptomics will give much more functional insight than just a list of taxa, it is disappointing to see that the results are limited to microbial taxon prevalence and not any functional analysis.

Based on this paper, Viome’s claims that their test can connect the microbiome transcriptome to a subject’s diet – let alone give dietary advice – appear to be very far-fetched.

Specific / detailed comments

1. Introduction: Reference 1, Nasca 2018, does not appear to be about the microbiome but about Acetyl-L-carnitine deficiency in people with depression
2. “16S gene” should be “16S rRNA gene”, at least the first mention in the manuscript
3. Not all 16S sequencing uses a short piece, in several cases one can identify to species level, depending on length of gene fragment used
4. “In addition, it misses some bacteria, most archaea, and all eukaryotic organisms and viruses [19].” Actually, reference 19 shows that great archaeal diversity can be captured using archeaon specific 16S primers (which is still 16S sequencing) – as long as you use different primers. Also this reference does not support the statement that 16S sequencing misses eukaryotic organisms and viruses.
5. Table 1 is not very objective (see above). The last 2 rows appear to say the same thing; statement in the right/bottom cell is too strong. Top right cell: only if metabolically active. 
6. Results: the first section (“Validation…”) might be better moved to the Methods.
7. Figure 1. It is not quite clear what the input here was. The RNA yield is given, but how were the input amounts relative to each other? Were equal number of cells added, or equal amount of turbidity? Were samples normalized for e.g. genome size?
8. “Ambient sample transportation”: The statement “There has been unfounded criticism of metatranscriptomics ” does not appear to be very scientific, and it lacks a reference.
9. Figure 2: It is not clear which of the peaks shows the RNA. The peaks in B are much lower; it would be better to show both panels at the same Y-axis scale. Also, if these are two “representative” samples, did they contain the same original sample?
10. Page 6: “Viomega eliminates STSC altogether” is not true – while it is low, it is not 0, as shown in Figure 3, so this needs to be reworded.
11. Figure 3: “Cross talk” is a very technical term – the figure legend should define how this is measured (text in Results was more helpful).
12. Page 7: “more than 1,000 fold lower ” – this data is not shown – either provide a reference or do the experiment.
13. Page 7, “Background contamination”. “Since any metagenomic or metatranscriptomic analysis identifies all taxa in a sample” is only true for live, transcribing organisms.
14. This section also states that background contamination would be picked up by the PPC sample (positive control) but is much more likely to be detected by the negative control sample. However, the study does not show how many reads were detected in the NC samples. Could the authors add that data? The Salter paper clearly shows that background taxa are rarely detected in samples with high amount of “real” taxa – they only show up when there is very low amount of real nucleic acid to detect.
15. What is Table 2 showing? Number of contaminating reads in PPC samples? More detail is needed here. How can these 1.4 reads per sample be distinguished from the “Sample to Sample Cross Talk” reads? Both Figure 3 as well as Table 2 appear to be studying the same thing, that is the number of reads that were assigned to different taxa than included in the PPC mixture. Could the authors clarify the difference?
16.  “Findings from the Viomega taxonomic classification”. The main text says that Tables S1-S3 include the top 100 taxa, but this appears incorrect. E.g., S1 lists 2723 taxa (strain level), so there are many more taxa than 100.
17. Table 4
    1. The table lists 12 KEGGS, so it is not a top 10 as mentioned in the text
    2. The top 10 KEGGS (table 4) and the top listings in Table S4 do not match. K00540 (#5 in Table S4) K03296 (#7) and K06950 (#10) are not present in Table 4. In contrast, K03737 (#3 in table 4), K00975 (#8), K00705 (#9) are not present in the top 10 in Table S4.
    3. Tables 4/S4: a short description of what these functions entail would be welcome.
    4. KEGG K00936 appears to be a two-component system, sensor histidine kinase PdtaS [EC:2.7.13.3] – not E2.7.3.-[EC:2.7.3.-] as denoted.
18. Page 11. Subject 11’s stool appears to show some clustering per stool section (1/2/3).  “Biological replicates were indistinguishable from the technical replicates.” might therefore be incorrect; some statistical analysis is needed (.e.g Lin’s correlation) to see which factor (technical vs biological replicate vs subject) is the strongest.
19. Page 11. “While there have been claims of large intra-sample variability” – this refers to a paper (#34) that tested samples from different persons and different anatomical sites which – not surprisingly – were found to vary. However, in the current study, the authors tests biological and technical replicates, something that was not tested in Reference 34.
20. Tables S1-S3: Several taxa appear to behave very differently on strain/species/genus level.

Examples where genus-level prevalence is much lower than expected based on strain prevalence:

Eggerthella
Strain: Eggerthella lenta 1_1_60AFAA, at 97.08% prevalence
Species: Eggerthella lenta at 91.76%
Genus: Eggerthella at 61.12%
Veillonella
Strain: Veillonella dispar A at 92.34
Species: Veillonella dispar at 88.99%
Genus: Veillonella at 78.62%
Entamoeba:
Strain: Entamoeba nuttalli P19 at 9.85% / Entamoeba dispar SAW760 0.74%
Species: Entamoeba nuttalli: 9.87% / Entamoeba dispar 0.74%
Genus: Entamoeba: 0.79%

In other examples, taxa with high prevalence at genus level seem to disappear at species or strain level:

Lactococcus
Strain: Lactococcus piscium MKFS47 at 0.99% / Lactococcus raffinolactis NBRC 100932 at 0.23%
Species: Lactococcus piscium at 0.98%
Genus: Lactococcus at 96.88%
Escherichia
Strain level: Escherichia coli isolate 15 at 37.8%; Escherichia coli strain LS5218 at 9.5%, Escherichia coli M17 at 7.7%.
Species level: Escherichia coli at 27.40%
Genus level: Escherichia: 85.92% prevalence
Saccharomyces
Strain: Saccharomyces sp. ‘boulardii’ strain unique28 is present at 1.82% of samples, as the most prevalent Saccharomyces strain. Also Saccharomyces cerevisiae S288C at 0.80%.
Species level: S. cerevisiae is present in 3.9% of samples.
Genus level: Saccharomyces it is present in 25.44%.
Streptococcus
Strain: Streptococcus sp. 263_SSPC 5.56% / Streptococcus mutans U138 at 1.62%
Species: Streptococcus thermophilus 28.15% / Streptococcus mutans 9.92% / Streptococcus parasanguinis 7.54%
Genus: Streptococcus 90.47%
Salmonella
Strain: Salmonella enterica subsp. enterica strain SE696A 2.30%; Salmonella enterica subsp. enterica strain ADRDL-LA-5-2013 2.28% / Salmonella enterica subsp. enterica serovar Typhimurium strain 1.52%
Species: Salmonella enterica 11.71%
Genus: Salmonella 15.61%

(Posting for our new team member, Zehra Tüzün Güvener.)

Good Morning! Highlights from Monday’s digest include a widely distributed efflux pump in saprophytic soil bacteria, genomic analysis of plant-growth-promoting rhizobacteria, identification of novel mammalian viruses from bats, rectal swabs to study gut microbiomes and TrueBiome’s solution to reduce variability in animal experiments.

General Microbiology

Efflux pumps in Chromobacterium species and their involvement in antibiotic tolerance and survival in a co-culture competition model – Benomar et al. – BioRxiv

Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli – Joers et al. –  BioRxiv
Human oral microbiome

Current Understanding of the Gut Microflora in Subjects with Nutrition-Associated Metabolic Disorder Such as Obesity and/or Diabetes: Is There Any Relevance with Oral Microflora? – Yumoto et al. –  Current Oral Health Reports
Human skin microbiome

Scalp bacterial shift in Alopecia areata – Pinto et al. –  PLOS ONE
Human gut microbiome

Factors influencing the gut microbiome in children: from infancy to childhood – Kumbhare et al. – Journal of Biosciences
Animal microbiomes

Defining Escherichia coli as a health-promoting microbe against intestinal Pseudomonas aeruginosa – Christofi et al. – BioRxiv

Glyphosate, but not its metabolite AMPA, alters the honeybee gut microbiota – Blot et al. – PLOS ONE
Plant, root and soil microbiome

**MiniReview: Gene mobility in microbiomes of the mycosphere and mycorrhizosphere –role of plasmids and bacteriophages – Pratama et al. –  FEMS Microbial Ecology

**Review: Response of microbial communities to biochar-amended soils: a critical review – Palansooriya et al. –  Biochar

The effects of soil phosphorous content on microbiota are driven by the plant phosphate starvation response – Finkel et al. – – BioRxiv

Comparative genomic analysis of Bacillus paralicheniformis MDJK30 with its closely related species reveals an evolutionary relationship between B. paralicheniformis and B. licheniformis – Du et al. – BMC Genomics

Are drivers of root-associated fungal community structure context specific? – Alzarhani et al. –  The ISME Journal
Water and extremophile microbiome

Impact of NaCl, nitrate and temperature on the microbial community of a methanol-fed, denitrifying marine biofilm – Villemur et al.  – BioRxiv

Metabolic potential of uncultured bacteria and archaea associated with petroleum seepage in deep-sea sediments – Dong et al. – Nature Communications
Probiotics / prebiotics

Screening of single or combined administration of 9 probiotics to reduce ammonia emissions from laying hens – Mi et al. – Poultry Science
Phages and viruses

A viral metagenomic survey identifies known and novel mammalian viruses in bats from Saudi Arabia – Mishra et al. – PLOS ONE
Techniques

Metaproteomics of Freshwater Microbial Communities – Russo et al. –  Mass Spectometry of Proteins (Methods in Molecular Biology)

Functional annotation of orthologs in metagenomes: a case study of genes for the transformation of oceanic dimethylsulfoniopropionate – Gonzales et al. –  The ISME Journal

Quantitative and qualitative evaluation of the impact of the G2 enhancer, bead sizes and lysing tubes on the bacterial community composition during DNA extraction from recalcitrant soil core samples based on community sequencing and qPCR – Gobbi et al. — PLOS ONE

Usability of rectal swabs for microbiome sampling in a cohort study of hematological and oncological patients – Biehl et al. –  PLOS ONE
Microbes in the news

** Report – North America Human Microbiome Market to 2025 – Regional Analysis and Forecasts by Product, Disease, Application, and Country –  Research and Markets
Microbes on the market

** Newsletter – Addressing experimental variability: Taconic launches TruBiome – Outsourcing-Pharma.com

Good morning everyone! In today’s microbiome digest, we have multiple research and review articles focusing on the role of microbiome in Prostate Cancer, Breast Cancer, Gastric Cancer, Allergy, Gut-Brain axis, Schizophrenia, Paediatric Crohn’s Disease, Nonalcoholic Fatty Liver Disease, Alveolar Osteitis, Renal Failure, Cholecystokinin Expression, Lipid Metabolism Disorder, Regulation of Blood Pressure and an independent section on Fecal Microbiota Transplantation. Additionally, two very interesting metagenomic studies have discovered specific microbial signature as well as link with choline degradation in the colorectal cancer patients. Enjoy reading.

General microbiome

Reivew: The Human Microbiota and Prostate Cancer: Friend or Foe? – Francesco Massari – Cancers

Microbiome—Microbial Metabolome—Cancer Cell Interactions in Breast Cancer—Familiar, but Unexplored – Edit Miko – Cells

Review: Fighting AMR in the Healthcare Environment: Microbiome-Based Sanitation Approaches and Monitoring Tools – Maria D’Accolti – Molecular Sciences

Gut microbiome

Gut microbial dysbiosis is associated with allergen-specific IgE responses in young children with airway allergies – Chih-Yung Chiu – World Allergy Organization Journal

Review: The gut virome: the ‘missing link’ between gut bacteria and host immunity? – Indrani Mukhopadhya – Therapeutic Advances in Gastroenterology

Regulatory T Cells and Plasmacytoid Dendritic Cells Within the Tumor Microenvironment in Gastric Cancer Are Correlated With Gastric Microbiota Dysbiosis: A Preliminary Study – Zongxin Ling – Frontiers in Immunology

Review: Impact of Different Types of Diet on Gut Microbiota Profiles and Cancer Prevention and Treatment – Rainer J. Klement – Medicina

Review: Glutamatergic Signaling Along The Microbiota-Gut-Brain Axis – Andreina Baj – Molecular Sciences

Investigation of the Gut Microbiome in Patients with Schizophrenia and Clozapine-Induced Weight Gain: Protocol and Clinical Characteristics of First Patient Cohorts –Gorbovskaya I. –  Neuropsychobiology

Review: Sex Differences in Gut Microbiota – Yong Sung Kim – The World Journal of MEN’s HEALTH

The Microbiome in Paediatric Crohn’s Disease—A Longitudinal, Prospective, 
Single-Centre Study – Shivani Kansal – Journal of Crohn’s and Colitis

A gut microbiome signature for cirrhosis due to nonalcoholic fatty liver disease – Cyrielle Caussy – Nature Communications

Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation – Andrew Maltez Thomas – Nature Medicine

Meta-analysis of fecal metagenomes reveals global microbial signatures that are specific for colorectal cancer – Jakob Wirbel – Nature Medicine

Ceftriaxone and cefotaxime have similar effects on the intestinal microbiota in human volunteers treated by standard doses regimens – Charles Burdet – Antimicrobial Agents and Chemotherapy

Human respiratory microbiome

Mycobiome Sequencing and Analysis Applied to Fungal Community Profiling of the Lower Respiratory Tract During Fungal Pathogenesis – Lisa R. McTaggart – Frontiers in Microbiology

Reproductive tract microbiome

Identification and evaluation of the microbiome in the female and male reproductive tracts – Rivka Koedooder – Human reproduction update

Skin microbiome

Mast cell recruitment is modulated by hairless skin microbiome – Chia-Chi Wu – The Journal of Allergy and Clinical Immunology

Oral microbiome

A metagenomic study of patients with alveolar osteitis after tooth extraction. A preliminary case-control study – Laura Aguilar-Duran – Clinical Oral Investigations

Animal microbiome / Animal experiments

Interactions Between Commensal Bacteria and Enteric Neurons, via FPR1 Induction of ROS, Increase Gastrointestinal Motility in Mice – Bindu Chandrasekharan – Gastroenterology

Danshen can interact with intestinal bacteria from normal and chronic renal failure rats – Hongdie Cai – Biomedicine and Pharmacotherapy

Review: A place for taxonomic profiling in the study of the coral prokaryotic microbiome – Alejandra Hernandez-Agreda – FEMS Microbiology Letters

Fructose malabsorption induces cholecystokinin expression in the ileum and cecum by changing microbiota composition and metabolism – Xufei Zhang – THE FASEB JOURNAL

Spinach consumption ameliorates the gut microbiota and dislipaemia in rats with diet-induced non-alcoholic fatty liver disease (NAFLD) – Elvira-Torales LI – Food and Functions

Microbial Colonization in Adulthood Shapes the Intestinal Macrophage Compartment – Franziska Schmidt – Journal of Chron’s and Colitis

Feeding modes shape the acquisition and structure of the initial gut microbiota in newborn lambs – Yanliang Bi – Environmental Microbiology

Effects of dietary lipid sources on the intestinal microbiome and health of golden pompano (Trachinotus ovatus) – Cuihong You – Fish & Shellfish Immunology

Gut microbiota characterization and lipid metabolism disorder found in PCB77-treated female mice – Chi Y – Toxicology

Changes of gut microbiota between different weight reduction programs – Belle Yanyu Lin – Surgery for Obesity and Related Diseases

Microbiome-metabolomic analysis of the impact of Zizyphus jujuba cv. Muzao polysaccharides consumption on colorectal cancer mice fecal microbiota and metabolites – Xiaolong Ji – International Journal of Biological Macromolecules

Fecal Microbiota Transplantation

Critical Role of the Interaction Gut Microbiota – Sympathetic Nervous System in the Regulation of Blood Pressure – Marta Toral – Frontiers in Physiology

Faecal microbiota transplantation for the decolonisation of antibiotic-resistant bacteria in the gut: a systematic review and meta-analysis – Vera Tavoukjian – The Journal of Hospital Infection

Prebiotics/Probiotics

Effect of a multistrain probiotic (Lactoflorene^®Plus) on inflammatory parameters and microbiota composition in subjects with stress-related symptoms – Sara Soldi – Neurobiology of Stress

Probiotics, prebiotics, and low FODMAP diet for irritable bowel syndrome – What is the current evidence? – Soo Liang Ooi – Complementary Therapies in Medicine

Water and extremophile microbiome

Microbial Community of Saline, Alkaline Lakes in the Nebraska Sandhills Based on 16S rRNA Gene Amplicon Sequence Data – Nicole A. Fiore – Microbiology Resource Announcements

Methods

A Customized At-Home Stool Collection Protocol for Use in Microbiome Studies Conducted in Cancer Patient Populations – Stephanie R. Hogue – Microbial Ecology

Effect of storage and DNA extraction method on 16S rRNA-profiled fecal microbiota in Japanese adults – Yuki Kawada – Journal of Clinical Biochemistry and Nutrition

Bioinformatics

𝑊∗𝑑Wd∗-test: robust distance-based multivariate analysis of variance – Bashir Hamidi – Microbiome

Identification and reconstruction of novel antibiotic resistance genes from metagenomes – Fanny Berglund – Microbiome

In today’s microbiome digest, multiple research and review articles focusing on the role of host-associated microbiomes in various diseases including Type 2 Diabetes, Obesity, Urolithiasis, Bronchial Asthma, Cancer Immunotherapy, Hepatic Glycan Storage, Uveitis, Schizophrenia and the Development of the immune system. Enjoy reading.

General microbiome

Harnessing microbiota interactions to produce bioactive metabolites: communication signals and receptor proteins – Juan F Martin – Current Opinion in Pharmacology

Review: Prevention of allergy with diverse and healthy microbiota:an update – Diana Chernikova – Current Opinion in Pediatrics

State of the Art: Pharmacomicrobiomics: The holy grail to variability in drug response? – Anukriti Sharma – Clinical Pharmacology and Therapeutics

Gut microbiome

The alteration of gut microbiota in newly diagnosed type 2 diabetic patients – Pei-Chi Chen – Nutrition

Defining Dysbiosis in Patients with Urolithiasis – Anna Zampini – Scientific Reports

Review: Gut Microbiota, Host Organism, and Diet Trialogue in Diabetes and Obesity – Veronica Lazar – Frontiers in Nutrition

Metabolic activity of intestinal microflora in patients with bronchial asthma – Vladimir Irashkin – Clinics and Practice

Pharmacologic and Nonpharmacologic Therapies for the Gut Microbiota in Type 2 Diabetes – Robert Caesar – Canadian Journal of Diabetes

Review: Modulation of cancer immunotherapy efficacy by gut microbiota – Huo S – Discovery Medicine

Hepatic glycogen storage diseases are associated to microbial dysbiosis – Karina Colonetti – PLOS ONE

Implicating Dysbiosis of the Gut Fungal Microbiome in Uveitis, an Inflammatory Disease of the Eye – Rajagopalaboopathi Jayasudha – Immunology and Microbiology

Cohort profile: The LoewenKIDS Study – life-course perspective on infections, the microbiome and the development of the immune system in early childhood – Cornelia Gottschick – International Journal of Epidemiology

Case Report: Decreased Clostridium Abundance after Electroconvulsive Therapy in the Gut Microbiota of a Patient with Schizophrenia – Misako Kanayama – Case Reports in Psychiatry

Reproductive tract microbiome

Effects of oral probiotic supplements on vaginal microbiota during pregnancy: a randomised, double‐blind, placebo‐controlled trial with microbiome analysis – S Husain – BJOG 

Skin microbiome

Review: The Cutaneous Microbiome: Implications for Dermatology Practice – Megan A. Sander

Animal microbiome / Animal experiments

Intestinal microbiota of broilers submitted to feeding restriction and its relationship to hepatic metabolism and fat mass: Fast-growing strain – Raquel Lunedo – Journal of Animal Physiology and Animal Nutrition

Phytate degradation, intestinal microbiota, microbial metabolites and immune values are changed in growing pigs fed diets with varying calcium–phosphorus concentration and fermentable substrates – Charlotte Maria Elisabeth Heyer – Journal of Animal Physiology and Animal Nutrition

Review: The importance of scale in comparative microbiome research: New insights from the gut and glands of captive and wild lemurs – Lydia K. Greene – American Journal of Primatology

A dietary isothiocyanate-enriched moringa (Moringa oleifera) seed extract improves glucose tolerance in a high-fat-diet mouse model and modulates the gut microbiome – Asha Jaja-Chimedza – Journal of Functional Foods

Musca domestica Cecropin (Mdc) Alleviates Salmonella typhimurium-Induced Colonic Mucosal Barrier Impairment: Associating With Inflammatory and Oxidative Stress Response, Tight Junction as Well as Intestinal Flora – Lun Zhang – Frontiers in Microbiology

The Effects of Unfermented and Fermented Cow and Sheep Milk on the Gut Microbiota – Elizabeth A. Rettedal – Frontiers in Microbiology

Water and extremophile microbiome

Microbiota of the Hickey Run Tributary of the Anacostia River – Robin Cagle – Microbiology Resource Announcements

Bioinformatics

Review: Statistical Analysis of Metagenomics Data – M. Luz Calle – Genomics and Informatics

Good morning everyone! In today’s microbiome digest, we have multiple research and review articles about the role of microbiome in various diseases including neurodevelopmental, biliary Cholangitis, obesity-associated CRC, bariatric surgery, atopic dermatitis, schizophrenia, respiratory tract infection and autoimmunity, and an independent section on Fecal Microbiota Transplantation. Enjoy reading.

General microbiome

Talking about a microbiome revolution – John F. Cryn – Nature Microbiology

Review: Crosstalk between neutrophils and the microbiota – Dachuan Zhang – Blood

 Gut microbiome

Alteration of gut microbiota by a westernized lifestyle and its correlation with insulin resistance in non‐diabetic Japanese men – Mami Yamashita – Journal of Diabetes Investigation

Association of the Infant Gut Microbiome With Early Childhood Neurodevelopmental Outcomes – Joanne E. Sordillo – JAMA Netw Open

Comprehensive Analysis of Serum and Fecal Bile Acid Profiles and Interaction with Gut Microbiota in Primary Biliary Cholangitis – Weihua Chen – Clinical Reviews in Allergy & Immunology

Gut microbiome meta-analysis reveals dysbiosis is independent of body mass index in predicting risk of obesity-associated CRC – K Leigh Greathouse – BMJ Open Gastroenterology

Gut microbiota specific signatures are related to the successful rate of bariatric surgery – Gutiérrez-Repiso C – American Journal of Transplantation Research

Differences in Systemic IgA Reactivity and Circulating Th Subsets in Healthy Volunteers With Specific Microbiota Enterotypes – Christina Grosserichter-Wagener – Frontiers in Immunology

Gut microbiota profile in children affected by atopic dermatitis and evaluation of intestinal persistence of a probiotic mixture – Sofia Reddel – Scientific Reports

Prebiotic effect of predigested mango peel on gut microbiota assessed in a dynamic in vitromodel of the human colon (TIM-2) – Sonia G. Sáyago-Ayerdi – Food Research International

Review: The gut microbiota promotes the pathogenesis of schizophrenia via multiple pathways – Xiuxia Yuan – Biochemical and Biophysical Research Communications

Evidence for an association of gut microbial Clostridia with brain functional connectivity and gastrointestinal sensorimotor function in patients with irritable bowel syndrome, based on tripartite network analysis – Jennifer S. Labus – Microbiome

A multi-omic cohort as a reference point for promoting a healthy human gut microbiome – Zhuye Jie – bioRxiv

Human respiratory microbiome

The microbiological characteristics of lower respiratory tract infection in patients with neuromuscular disorders: An investigation based on a multiplex polymerase chain reaction to detect viruses and a clone library analysis of the bacterial 16S rRNA gene sequence in sputum samples – Masato Ogawa – Journal of Microbiology, Immunology and Infection

Vaginal micorbiome

Cervicovaginal microbiota and local immune response modulate the risk of spontaneous preterm delivery – Michal A. Elovitz – Nature Communications

Animal microbiome / Animal experiments

Autoimmunity-Associated Gut Commensals Modulate Gut Permeability and Immunity in Humanized Mice – Baskar Balakrishnan – Military Medicine

Microbe and host interaction in gastrointestinal homeostasis – Rachael Horne – Psychopharmacology

Broiler gut microbiota and expressions of gut barrier genes affected by cereal type and phytogenic inclusion – VasileiosParaskeuas – Animal Nutrition

Manipulation of gut microbiota blunts the ventilatory response to hypercapnia in adult rats – Karen M. O’Connor – EbioMedicine

Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice – Lihua Han – Nutrients

Effects of gut-derived endotoxin on anxiety-like and repetitive behaviors in male and female mice – Christopher T. Fields – Biology of Sex Differences

Fecal Microbiota Transplantation

Recent Research on Fecal Microbiota Transplantation in Inflammatory Bowel Disease Patients – Monika Fischer – Gastroenterology and Hepatology

Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes – Wei Wang – Nature Communications

Do we really understand how faecal microbiota transplantation works? Authors’ reply – Lito E. Papanicolas – EbioMeidicine

Water and extremophile microbiome

Mobile antibiotic resistome in wastewater treatment plants revealed by Nanopore metagenomic sequencing – You Che – Microbiome

Evolution of microbial community and drug resistance during enrichment of tetracycline-degrading bacteria – Qiang Wang – Ecotoxicology and Environmental Safety

Bioinformatics

A generic multivariate framework for the integration of microbiome longitudinal studies with other data types – Antoine Bodein – bioRxiv