March 26, 2015

General microbiome

Note: not about metagenomics but 16S rRNA gene: The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies – J Paul Brooks – BMC Microbiology

Human respiratory microbiome

Spatial Variation in the Healthy Human Lung Microbiome and the Adapted Island Model of Lung Biogeography – Robert P. Dickson – Annals ATS

A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients – Mohammad A. Tariq – Frontiers in Microbiology

Perspective: Meningitis in adolescents: the role of commensal microbiota – James W.B. Moir – Trends in Microbiology

Human gut microbiome

An n=1 case report of a child with autism improving on antibiotics and a father’s quest to understand what it may mean – John Rodakis – Microbial Ecology of Health and Disease

Animal microbiome

Sponges: Culturable associated-bacteria of the sponge Theonella swinhoei show tolerance to high arsenic concentrations – Ray Keren – Frontiers in Microbiology

Soil, sediment, and plant root microbiome

Community Dynamics of Arbuscular Mycorrhizal Fungi in High-Input and Intensively Irrigated Rice Cultivation Systems – Yutao Wang – Applied and Environmental Microbiology

Analysis of methane-producing and metabolizing archaeal and bacterial communities in sediments of the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes – Zhichao Zhou – Frontiers in Microbiology

Isolation and characterization of culturable bacteria from bulk soil samples and the rhizosphere of arid-adapted Tylosema esculentum (Burchell). A. Schreiber (Marama bean) in Namibia – Olaf S. Kandjimi – African Journal of Biotechnology

Water microbiome

Prokaryotic assemblages and metagenomes in pelagic zones of the South China Sea – Ching-Hung Tseng – BMC Genomics

Spatial distribution of bacterial communities driven by multiple environmental factors in a beach wetland of the largest freshwater lake in China – Xia Ding- Frontiers in Microbiology

Review: Advancing the science of microbial symbiosis to support invasive species management: a case study on Phragmites in the Great Lakes – Kurt P. Kowalski – Frontiers in Microbiology

Extremophile microbiome (by special request!)

Colonization of plant substrates at hydrothermal vents and cold seeps in the northeast Atlantic and Mediterranean and occurrence of symbiont-related bacteria – Kamil M. Szafranski – Frontiers in Microbiology

Out of the dark: transitional subsurface-to-surface microbial diversity in a terrestrial serpentinizing seep (Manleluag, Pangasinan, the Philippines) – Kristin M. Woycheese – Frontiers in Microbiology

Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China – Brian P. Hedlund – Frontiers in Microbiology

Fatty Acids Profile of Microbial Populations in a Mining Reclaimed Region Contaminated with Metals: Relation with Ecological Characteristics and Soil Respiration – Ramya Narendrula and Kabwe K. Nkongolo – Journal of Bioremediation & Biodegradation

Bioreactor microbiome

Nitrogen addition, not initial phylogenetic diversity, increases litter decomposition by fungal communities – Anthony S. Amend – Frontiers in Microbiology

Food microbiology

Lipidomics as an important key for identification of beer-spoilage bacteria – Tomáš Řezanka – Letters in Applied Microbiology

Metabolomics

Combining reverse genetics and NMR-based metabolomics unravels trypanosome-specific metabolic pathways – Frédéric Bringaud – Molecular Microbiology

Resource niche overlap promotes stability of bacterial community metabolism in experimental microcosms – Ellard R. Hunting – Frontiers in Microbiology

Techniques

Enriching distinctive microbial communities from marine sediments via an electrochemical-sulfide-oxidizing process on carbon electrodes – Shiue-Lin Li and Kenneth H. Nealson – Frontiers in Microbiology

Microbes in presentations

Video: Dissertation seminar for Suzanne Ishaq – comparative analysis of the moose rumen microbiota

Video: Vance Kramer of Syngenta discussing microbiome research and comparing soil microbe communities across the US, Europe, and Brazil.

Video: Dr. Jan Jones from AgBiome discussing the plant-soil microbiome

Microbes in the news
Hidden Life Forms: Investigating Microbial Diversity on Our Bodies and in Our Homes – Jennifer Cutraro – New York Times

My uBiome Sleep Hacking Update – Alexandra Carmichael – uBiome

Antimicrobial CRISPR-Cas systems may be better weapons against bacteria than antibiotics – The Conversation

Secret Science Club Post-Lecture Recap: Big Data, Genomics and Urban Microbiomes – Big Bad Bald Bastard

Antibiotics For Gut Bacteria Show Surprising Results In Treating Autism – Dianne Depra – Tech Times

Harmless bacteria may be helpful against meningococcal outbreaks – Eureka Alert

New Software Offers Improved Read-based Identification in Metagenome Studies – Andrew P. Han – Genome Web

Not sure which one, but here it is: I am the bacterium that lives in your intestine – MicrobeWorld

FMTUpdate on OpenBiome – Paul Levy – Not Running A Hospital

Workshop on Planetary Protection Knowledge Gaps for Human Extraterrestrial Missions (Intro) – Jenna Lang – Microbe.net

Microbes and art

A Dress Made From Beer And Bacteria: C’est Chic Or C’est Ick? – Charles Poladian – IB Times

Science, publishing, and career

Developing microbiological learning materials for schools: best practice – James Redfern – FEMS Microbiology Letters

Sequencing and characterizing the genome of Estrella lausannensis as an undergraduate project: training students and biological insights – Claire Bertelli – Frontiers in Microbiology

Calling attention to poor speaker gender ratio – even when it hurts – Jonathan Eisen – The Tree of Life

The 5 Biases Pushing Women Out of STEM – Joan C. Williams – Harvard Business Review

Bik’s Picks

Jurrassic Park, but then with more furWoolly mammoth could be revived after scientists paste DNA into elephant’s genetic code – Andrew Griffin – The Independent

The Fitness Guru of the Trucking Industry – The Atlantic

On Friday, half a pair of twins leaves for a year in space. Here’s why that’s awesome. – Rachel Feltman – Washington Post

[hr]

March 25, 2015

General microbiome

Introducing npj Biofilms and Microbiomes – Staffan Normark – NPJ Biofilms and Microbiomes – includes:

Clinical Implications of Basic Science Discoveries: Immune Homeostasis and the Microbiome—Dietary and Therapeutic Modulation and Implications for Transplantation – J. A. Fishman, and A. W. Thomson – American Journal of Transplantation

Human respiratory microbiome

Clinical Outcomes and Microbiological Characteristics of Severe Pneumonia in Cancer Patients: A Prospective Cohort Study – Ligia S. C. F. Rabello – PLOS ONE

Human gut microbiome

Subsistence strategies in traditional societies distinguish gut microbiomes – Alexandra J. Obregon-Tito – Nature Communications

Variation in rural African gut microbiomes is strongly shaped by parasitism and diet – Elise R Morton – BioRxiv

Some problems with JAMA website, not sure if link works: Association of Gastrointestinal Symptoms With Autism – JAMA Psychiatry

Contrasting effects of fresh and fermented kimchi consumption on gut microbiota composition and gene expression related to metabolic syndrome in obese Korean women – Kyungsun Han – Molecular Nutrition & Food Research

Review: Alcohol and gut microbiota – the possible role of gut microbiota modulation in the treatment of alcoholic liver disease – G. Vassallo – Alimentary Pharmacology & Therapeutics

Perspective: Studies of the Gut: Overhyped or Underappreciated? – Tom Bartlett – The Chronicle of Higher Education

Review: Microbial biofilms and the human intestinal microbiome – Willem M de Vos – NPJ Biofilms and Microbiomes

Review: Microbial endocrinology: host-bacteria communication within the gut microbiome – Sara Sandrini – Journal of Endocrinology

Dead body microbiome

Potential Use of Bacterial Community Succession in Decaying Human Bone for Estimating Postmortem Interval – Franklin E. Damann – Journal of Forensic Sciences

Life after death: shallow-water Mediterranean invertebrate communities associated with mammal bones – Sergi Taboada – Marine Ecology

Animal microbiome

Monkeys: Adenovirus and Herpesvirus Diversity in Free-Ranging Great Apes in the Sangha Region of the Republic of Congo – Tracie A. Seimon – PLOS ONE

Birds (not birds and bees): Pathogenic bacteria and timing of laying – Anders Pape Møller – Ecology and Evolution

Arthropods: Metagenomic Profile of the Viral Communities in Rhipicephalus spp. Ticks from Yunnan, China – Han Xia – PLOS ONE

Nematodes: Associated bacteria of different life stages of Meloidogyne incognita using pyrosequencing-based analysis – Yi Cao – Journal of Basic Microbiology

Corals: Microbial Communities Associated with Healthy and White Syndrome-Affected Echinopora lamellosa in Aquaria and Experimental Treatment with the Antibiotic Ampicillin – David Smith – PLOS ONE

Water microbiome

Plant pathogenic bacteria in open irrigation systems: what risk for crop health? – J. R. Lamichhane and C. Bartoli – Plant Pathology

Elements of metacommunity structure and community-environment relationships in stream organisms – Jani Heino – Freshwater Biology

Opportunistic Pathogens and Elements of the Resistome that Are Common in Bottled Mineral Water Support the Need for Continuous Surveillance – Maria Fernanda Falcone-Dias – PLOS ONE

Bioinformatics

MUSiCC: a marker genes based framework for metagenomic normalization and accurate profiling of gene abundances in the microbiome – Ohad Manor and Elhanan Borenstein – Genome Biology

Average genome size estimation improves comparative metagenomics and sheds light on the functional ecology of the human microbiome – Stephen Nayfach and Katherine S Pollard – Genome Biology

Novel R Pipeline for Analyzing Biolog Phenotypic Microarray Data – Minna Vehkala – PLOS ONE

Ecology

Probabilistic Models to Describe the Dynamics of Migrating Microbial Communities – Joanna L Schroeder – PLOS ONE

Scanning the Landscape of Genome Architecture of Non-O1 and Non-O139 Vibrio cholerae by Whole Genome Mapping Reveals Extensive Population Genetic Diversity – Carol Chapman – PLOS ONE

Archaea

Phylogeny and Taxonomy of Archaea: A Comparison of the Whole-Genome-Based CVTree Approach with 16S rRNA Sequence Analysis – Guanghong Zuo – Life

Techniques

Open Labware: 3-D Printing Your Own Lab Equipment – Tom Baden – PLOS Biology

Microbes in the news

Treponema are not “ancient” but absence from some human’s guts is very interesting – Jonathan Eisen – Tree of Life
Refers to:
Ancient bacteria found in hunter-gatherer guts – Ann Gibbons – Science

With recipes: Upcoming book by Justin and Erica Sonnenburg: “The Good Gut” – Amazon

The ‘intraterrestrials’: New viruses discovered in ocean depths – National Science Foundation

Martian Microbes Could Be Eating Carbon Monoxide From the Atmosphere – Popular Mechanics

Kids with Autism Are More Likely to Have Gastrointestinal Problems – Rachael Rettner – LiveScience

Examining the connection between infant gut bacteria and food allergies – HealthCanal

Repeated Use of Antibiotics May Raise Diabetes Risk – Agata Blaszczak-Boxe – LiveScience

Bacteria in bud prompts B.C. medical marijuana firm to recall product – Canadian Press

Twin astronauts provide Earth-bound control for orbital health study – Phys.org

Baylor College of Medicine: Certain makeup can cause bacterial infections – John Brannen – Click2Houston

The Bathroom Blast Radius (And Your Toothbrush) – Dollar Shave Club

The Antibiotics Problem in Meat – Olga Khazan – The Atlantic

Satire: Biotechnology pressure group calls for an end to Saccharomyces abuse – The Allium

Science, publishing, and career

The 2015 Salary Survey Is Here – The Scientist

Women still find it tough to reach the top in science – Sharon Bell – The Conversation

Bik’s Picks

Ancient Egyptian skeleton shows signs of breast cancer – Elahe Izadi – Washington Post

Unravelling the hidden ancestry of American admixed populations – Francesco Montinaro – Nature Communications

Short Circuit in Magnets Postpone Re-Launch of CERN Collider – InsightTicker

Why the world needs biodiversity – Scientists have engineered the food that will help save a starving, warming planet – Jack Aldwinckle – Quartz

[hr]

March 24, 2015

General microbiome

Review: Biomarkers for the 21st Century: Listening to the Microbiome – Rodney Reynolds Dietert* and Ellen Kovner Silbergeld – Toxicological Sciences

Nice overview [PDF]: FASEB Releases “The Human Microbiome: Your Own Personal Ecosystem” – FASEB

Microbiome of pregnancy and birth

Bacterial diversity and Clostridia abundance decrease with increasing severity of necrotizing enterocolitis – Valarie E McMurtry – Microbiome

Human vaginal microbiome

Vaginal Microbiota of Adolescent Girls Prior to the Onset of Menarche Resemble Those of Reproductive-Age Women – Roxana J. Hickey – mBio

Human gut microbiome

The detection of vector-borne-disease-related DNA in human stool paves the way to large epidemiological studies – Alpha Kabinet Keita – European Journal of Epidemiology

Animal and other models

A Modified R-Type Bacteriocin Specifically Targeting Clostridium difficile Prevents Colonization of Mice without Affecting Gut Microbiota Diversity – Dana Gebhart – mBio

Effects of Irradiation on Epithelial Wound Healing and Microbial Diversity in an
in-vitro Oral Mucosa Model – Tine De Ryck – Nuclear Medicine & Radiation
Therapy

Microbes on Mars? Carbon monoxide as a metabolic energy source for extremely halophilic microbes: Implications for microbial activity in Mars regolith – Gary M. King – PNAS USA

Animal microbiome

Rabbit: The bacterial communities associated with fecal types and body weight of rex rabbits – Bo Zeng – Scientific Reports

Plant microbiome

Lots of omics words in this book: Microbiomics: An Approach to Community Microbiology – Pankaj Sharma – In: PlantOmics

Soil and plant root microbiome

The Soil Microbiome Influences Grapevine-Associated Microbiota – Iratxe Zarraonaindia – mBio

Elevated CO2 shifts the functional structure and metabolic potentials of soil microbial communities in a C4 agroecosystem – Jinbo Xiong – Scientific Reports

Isolation of a significant fraction of non-phototroph diversity from a desert Biological Soil Crust – Ulisses Nunes da Rocha – Frontiers in Microbiology

Nucleotide Sequences of Giant Viruses Found in Soil Samples of the Mojave Desert, the Prairie, the Tundra and the Antarctic Dry Valleys – Csaba Kerepesi – ArXiv

Impacts of bulk soil microbial community structure on rhizosphere microbiomes of Zea mays – Matthew G. Bakker – Plant and Soil

Pyrosequencing Reveals Changes in Soil Bacterial Communities after Conversion of Yungas Forests to Agriculture – Marcela S. Montecchia – PLOS ONE

Priority effects during fungal community establishment in beech wood – Jennifer Hiscox – The ISME Journal

Water microbiome

Very cool, with longitudinal plots: The Source of the River as a Nursery for Microbial Diversity – Luiz Felipe Valter de Oliveira, Rogério Margis – PLOS ONE

Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps – Adrien Vigneron – Applied and Environmental Microbiology

Site history and edaphic features override the influence of plant species on microbial communities in restored tidal freshwater wetlands – Christine E. Prasse – Applied and Environmental Microbiology

Multivariate and Phylogenetic Analyses Assessing the Response of Bacterial Mat Communities from an Ancient Oligotrophic Aquatic Ecosystem to Different Scenarios of Long-Term Environmental Disturbance – Silvia Pajares – PLOS ONE

Physiological and evolutionary potential of microorganisms from the Canterbury Basin subseafloor, a metagenomic approach – Frédéric Gaboyer – FEMS Microbiology Ecology

Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source – Eleftheria Antoniou – Frontiers in Microbiology

Built environment microbiome

Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments – Christine Moissl-Eichinger – Scientific Reports

Effect of Monochloramine Treatment on the Microbial Ecology of Legionella and Associated Bacterial Populations in a Hospital Hot Water System – Julianne L. Baron – Systematic and Applied Microbiology

Non-culturable bioaerosols in indoor settings: impact on health and molecular approaches for detection – Pascale Blais-Lecours – Atmospheric Environment

Probiotics

Development and Utility of the FDA “GutProbe” DNA Microarray for Identification, Genotyping, and Metagenomic Analysis of Commercially Available Probiotics – Jennifer N. Patro – Journal of Applied Microbiology

Antibiotics

Global trends in antimicrobial use in food animals – Thomas P. Van Boeckel – PNAS USA

Metagenomics and bioinformatics

Grid-Assembly: An oligonucleotide composition-based partitioning strategy to aid metagenomic sequence assembly – Tarini Shankar Ghosh – Journal of Bioinformatics and Computational Biology

Multiplexed metagenome mining using short DNA sequence tags facilitates targeted discovery of epoxyketone proteasome inhibitors – Jeremy G. Owen – PNAS USA

Insight into the evolution and origin of leprosy bacilli from the genome sequence of Mycobacterium lepromatosis – Pushpendra Singh – PNAS USA

Metabolomics / Ecology

Species interactions differ in their genetic robustness – Lon M. Chubiz – Frontiers in Microbiology

Microbes in the news

With a picture you will never forget: Acne and the Microbiome: Christi Investigates – Alexandra Carmichael

Vineyard’s soil microbes shape grapes’ microbial community – Science Daily

Crohn’s Disease: Gut Microbial Collaborations Trigger Inflammatory Diseases – PR NewsWire

Feed the microbes so they don’t feed on you: Fiber-Famished Gut Microbes Linked to Poor Health – Katherine Harmon Courage – Scientific American

Harnessing The Microbiome To Curb Hunger – Chemical and Engineering News

New Research On Appetite-Suppressing Bacteria Could Help Fight Obesity – Anna Almendrala

The MinION Access Programme (MAP) for portable DNA sequencing – Science Daily

Viruses, Archaea Self-mutate to Survive – Laboratory Equipment

Engineering and waterborne bacteria in building water systems – Colum Ó Bric -EngineersJournal

Gut and Mood: The Microbiome in Anxiety and Depression – Nina Weisbroth – Translational Microbiome

Not so fast, FastTree – Darling lab

SatireEach bioinformatician to have their own personal short-read aligner by 2016 – The Science Web

Not about Dr. WhoThe Doctor Who Tested Infectious Bacteria On Himself, And Won A Nobel – Ria Misra

Comparing the genomes of the leprosy bacteria – Phys.org

#BacteriaHysteria: “Beer Pong” Associated with Frequent Bacterial Transmissions – Jacquelyn Gray – HCPlive

#BacteriaHysteria: Clean machines: how to look after your tech and avoid bacteria bombs – David Howell – TechRadar

Science and art

Workshop, April 6, 2015: Paint with Bacteria – GenSpace, New York City’s Community Biolab

Science, publishing, and career

Opinion: Lay summaries needed to enhance science communication – Lauren M. Kuehne and Julian D. Olden – PNAS USA

50 min podcast: Women in Oceanography: Lots of Students, Not So Many Professors – Heather Goldstone – Capeandislands.com

This is why you shouldn’t believe that exciting new medical study – Julia Belluz – Vox.com

Stubborn Pay Gap Is Found in Nursing – Catherine Saint Louis – Catherine Saint Louis – New York Times

It’s time to reboot bioinformatics education – Todd Harris

Bik’s Picks

Macaques Exhibit a Naturally-Occurring Depression Similar to Humans – Fan Xu – Scientific Reports

Earth’s most powerful physics machine gets back in action – Marcus Woo – Wired.com

Scientists want museums to cut their ties with David Koch – Science Recorder

[hr]

March 23, 2015

General microbiome

Perspective: Separating the microbiome from the hyperbolome – Fergus Shanahan – Genome Medicine

Human oral microbiome

Interkingdom networking within the oral microbiome – Angela H. Nobbs, Howard F. Jenkinson – Microbes and Infection

Poster: Composition, Taxonomy And Functional Diversity Of The Oropharynx Microbiome In Individuals With Schizophrenia And Controls – Eduardo Castro Nallar – FigShare

Water microbiome

Targeted diversity generation by intraterrestrial archaea and archaeal viruses – Blair G. Paul – Nature Communications

Microbes in the News

Your poop could be a literal goldmine of precious metals – Rachel Feltman – Washington Post

Chlorine use in sewage treatment could promote antibiotic resistance – Science Daily

50 min podcast: Dr. Seth Bordenstein: Seeing Science and Symbiosis Through the Lens of an Evolutionary Microbiologist – People behind the science

New strategy to protect healthy gut microbes from antibiotics – Science Daily

Human parasites found in medieval cesspit reveal links between Middle East and Europe – Science Daily

Special Programmed Microbes May Help You Beat Obesity, Vanderbilt University Study – BioSpace

Skin microbiome may hold answers to protect threatened gold frogs from lethal fungus – Science Daily

Surviving in Hostile Territory: UCSB scientists discover a mechanism of self-mutation in mysterious viruses and microbes from within the Earth – Julie Cohen – UC Santa Barbara

Soaring antibiotic use in animals fuels ‘super bug’ fears – Chris Arsenault – Reuters

MRSA Is More Widespread Than You Think – Jason Tetro – Huffington Post Canada

Wolbachia Bacteria Can Control Mosquitoes with Fewer Chemicals – Erin Weeks – Entomology Today

Science, publishing and career

Social Media and Internet Driven Study Recruitment: Evaluating a New Model for Promoting Collaborator Engagement and Participation – Chetan Khatri – PLOS ONE

University of North Georgia apologized and agreed to stop distributing a course catalog with an denigrating picture – Travis Gettys – Raw Story

Bik’s Picks – Ancient rocks edition

When A Meteorite Hits Snow It Forms A “Snow Carrot” Instead Of A Crater – Mika McKinnon – Space io9

Telling stories from stone tools – Bruce Bower – Science News

Double Impact Crater in Canada Formed in Two Separate Impacts – Space Daily

[hr]

March 19, 2015

Just a short digest today!

General microbiome

Review: The role of the microbiota in ageing: current state and perspectives – Denise B. Lynch – Wiley Interdisciplinary Reviews: Systems Biology and Medicine

Human gut microbiome / proteomics

Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota – Alessandro Tanca – Proteomics

Animal microbiome

AbaloneTemporal fluctuation in the abundance of alginate-degrading bacteria in the gut of abalone Haliotis gigantea over 1 year – Reiji Tanaka – Aquaculture Research

Soil microbiome

Native California soils are selective reservoirs for multidrug-resistant bacteria – Amanda C. Hollowell – Environmental Microbiology Reports

Techniques

Growing microbes in millifluidic droplets – Laurent Boitard – Engineering in Life Sciences

Other microbiology

Application of Whole-Genome Sequencing for Bacterial Strain Typing in Molecular Epidemiology – Stephen J. Salipante – Journal of Clinical Microbiology

Microbes in the news

Adventures in exploring the great indoors – Anne A. Madden – Fierer Lab

MO BIO Laboratories, Inc. presents the Second Annual MO BIO Microbiome Awards, offering more than $10,000 in prizes! – MoBio

uBiome — Explore Your Dental Microbiome – IndieGoGo
uBiome Launches World’s First Dental Citizen Science Campaign – Global Newswire

Humans Need ‘Stronger’ Breast-Milk Than Most Mammals – Brian Stallard – Nature World News

Interactive/videos: The good, the bad and the ugly in everyday bacteria – CBC news

Los Alamos scientists develop new method for metagenomic analysis – News Medical Net

This week’s  Google Plus Microbiology Journal club: Cheese Microbe Communities – GPlusMicroJC

Researchers have a gut feeling – could chronic pain be caused by bacteria? – Michael Brooks – New Statesman

MeMed’s blood test accurately distinguishes bacterial and viral infections, research shows – Medical Xpress

Turning the gut microbiome into a chat room – Tina Hesman Saey – ScienceNews

Science, publishing, and career

A grad school survival guide – Andrew Gaudet – Science

Bik’s Picks

The New York Times’ smartwatch cancer article is bad, and they should feel bad – Russell Brandom – The Verge

Unmasked: – What 10 million passwords reveal – about the people who choose them – WP engine

All other languages tired of Python’s sh!t – The Science Web
[hr]

March 17, 2015

Happy St. Patrick’s day! Enjoy the green microbial fermented fluids.

Human oral microbiome

Metagenomic sequencing reveals altered metabolic pathways in the oral microbiota of sailors during a long sea voyage – Weiwei Zheng – Scientific Reports

A Filifactor alocis-centered co-occurrence group associates with periodontitis across different oral habitats – Hui Chen – Scientific Reports

Review: Polymicrobial nature of chronic oral disease – Stuart Dashper – Microbiology Australia

Animal models

Intestinal Microbiota Composition Modulates Choline Bioavailability from Diet and Accumulation of the Proatherogenic Metabolite Trimethylamine-N-Oxide – Kymberleigh A. Romano – mBio

Animal microbiome

Social networks predict gut microbiome composition in wild baboons – Jenny Tung – eLIFE

Unfolding the secrets of coral–algal symbiosis – Nedeljka Rosic – The ISME Journal

Water microbiome

Presence of oxygen and aerobic communities from sea floor to basement in deep-sea sediments – Steven D’Hondt – Nature Geoscience

Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea – Mohd Ikram Ansari – Scientific Reports

Oceanographic structure drives the assembly processes of microbial eukaryotic communities – Adam Monier – The ISME Journal

Other microbial science

Microbial reflectance spectra: Surface biosignatures of exo-Earths: Remote detection of extraterrestrial life – Siddharth Hegde – PNAS USA

Viruses and phages

Membrane vesicles in natural environments: a major challenge in viral ecology – Nicolas Soler – The ISME Journal

Lytic phages obscure the cost of antibiotic resistance in Escherichia coli – Samuel J Tazzyman and Alex R Hall – The ISME Journal

Metagenomics

Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy – Masaki Shintani – Frontiers in Microbiology

Bioinformatics

Minimum entropy decomposition: Unsupervised oligotyping for sensitive partitioning of high-throughput marker gene sequences – A Murat Eren – The ISME Journal

Microbes in the news

Baboon friends swap gut germs – Science Daily

No limit to life in deep sediment of ocean’s “deadest” region – National Science Foundation

The microbiome of alcoholics may contribute to pathologies – American Microbiome Institute

Reflectance spectra from microbes: This Alien Color Catalog May Help Us Spot Life on Other Planets – Jesse Emspak – Smithsonian

New Data May Help Identify Extraterrestrial Life – William Large – Clapway

Science, publishing, and career

Competitive Science: Is Competition Ruining Science? – Ferric C. Fang and Arturo Casadevall – Infection and Immunity

Bik’s Picks – Marine Mammal Edition

Starving sea lion pups inundate Southern California rescue centers – Marty Graham – Reuters

Sciencespeak: Whale Pump. Whales can poop almost anywhere they want. – Brian Switek – National Geographic

Long-lost whale fossil sheds light on East Africa’s evolution – Bianca Nogrady – ABC Science

[hr]

The Vaginal Microbiome

An overview of large studies on the human vaginal microbiome. Please leave a comment if I missed an important paper, which is extremely likely. This is just a first draft, and this post is meant to be continuously updated.

Scientific papers, most recent first

The vaginal microbiome during pregnancy and the postpartum period in a European population – David A. MacIntyre – Scientific Reports – March 2015

  • Subjects: 42 British women with term delivery, sampled longitudinally, 3 ethnic groups
  • DNA extraction: bead-beating + QIAamp DNA Mini kit
  • Sequencing: V1-V2 region, MiSeq, 2,113,251 reads, 14088 reads/sample (rarefied to 1916?)

The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women – Roberto Romero – Microbiome – February 2014

  • Subjects: 32 non-pregnant women, 22 pregnant women who delivered on time
  • DNA extraction: Bead-beating, ZR Fecal DNA extraction kit (ZYMO Research)
  • Sequencing: V1-V2 region, 454 FLX Titanium, 2,946,507 reads, 2878 reads/sample

Diversity of the Vaginal Microbiome Correlates With Preterm Birth – Richard W. Hyman – Reproductive Sciences – January 2014

  • Subjects: 88 pregnant women (various ethnicities), 143 swabs
  • DNA extraction: QIAgen DNeasy Tissue and Blood Kit
  • Sequencing: 8F-1492R, clone library, Sanger sequencing, read from one end? not clear.
    Number of clones not clear?

Diverse Vaginal Microbiomes in Reproductive-Age Women with Vulvovaginal Candidiasis
Mu-Biao Liu – PLOS ONE – November 2013

  • Subjects: 95 women, some with BV or VVC, 226 swabs
  • DNA extraction: DNA Magnetic Sand Extract kit (BioEAsy,China)
  • Sequencing: V6 region, Illumina HiSeq 2000, 1,026,382 reads, 4,542 reads/sample

A Metagenomic Approach to Characterization of the Vaginal Microbiome Signature in Pregnancy – Kjersti Aagaard – PLOS ONE – June 2012

  • Subjects: 24 pregnant women, 60 non-pregnant controls, multiple timepoints/subject
  • DNA extraction: PowerSoil DNA Isolation Kit
  • Sequencing: V3-V5 region, 454 FLX Titanium, 2.5 million reads, 6837 reads/sample

$$ Temporal Dynamics of the Human Vaginal Microbiota – Pawel Gajer – Science Translational Medicine – May 2012

  • Subjects: 32 women, 937 samples
  • DNA extraction: Bead-beating with ZR Fecal DNA extraction kit (Zymo Research)
  • Sequencing: V1-V2 region, 454 Titanium, 2,522,080 reads, 2692 sequences/sample

Vaginal Microbiome and Epithelial Gene Array in Post-Menopausal Women with Moderate to Severe Dryness – Ruben Hummelen – PLOS ONE – November 2011

  • Subjects: 32 post-menopausal women, some sampled over 10 weeks
  • DNA extraction: Instagene (Bio-Rad)
  • Sequencing: V6 region, Illumina, 1,758,430, 17,410 reads/sample

Vaginal microbiome of reproductive-age women – Jacques Ravel – PNAS USA – March 2011

  • Subjects: 396 North American women representing four ethnic groups
  • DNA extraction: Bead beating combined with QIAamp DNA mini kit
  • Sequencing: V1-V2 region, 454 FLX, 897,345 good-quality reads, 2200 reads/sample

Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women – Xia Zhou – The ISME Journal – May 2007

  • Subjects: 144 women, 2 ethnicities
  • DNA extraction: not listed
  • Sequencing: T-RFLP, clone library , 6000 clones, 90 clones/sample

Microbes on the human vaginal epithelium – Richard W. Hyman – PNAS USA – April 2005

  • Subjects: 20 women
  • DNA extraction: QIAgen DNAeasy
  • Sequencing: 8F x 1492R, ~1000-2000 clones per subject

Review papers

The vaginal microbiome: rethinking health and diseases – Bing Ma – Annual Reviews in Microbiology – September 2013

Understanding vaginal microbiome complexity from an ecological perspective – Roxana J. Hickey – Translational Research – October 2012

A New Era of the Vaginal Microbiome: Advances Using Next-Generation Sequencing – Jennifer M. Fettweis – Chemistry & Biodiversity – May 2012

The vaginal microbiome in health and disease – Bryan A. White – Trends in Endocrinology & Metabolism – October 2011

The vaginal microbiome: new information about genital tract flora using molecular based techniques – RF Lamont – BJOG: An International Journal of Obstetrics & Gynaecology
– April 2011

News articles

Sweet Peach won’t make vaginas smell like fruit or taste of Diet Coke – Hannah Jane Parkinson – The Guardian – November 2014

What’s in Your Vagina? A healthy microbiome, hopefully. Moises Velasquez-Manoff – Slate – January 2013

Characterizing the “Healthy” Vagina – Jef Akst – The Scientist – May 2014

[hr]

March 16, 2015

After a busy day showing three motivated high school students how to extract DNA and detect microbes from shoe soles, floor tiles, and mouths, finally time to catch up with the literature. Be sure to scroll down for the Microbiome Game.

Conferences and other events

March 19th, 2PM ET: Cheese Microbe Communities – Google Hangouts

29 June – 1 July 2015: Exploring Human Host-Microbiome Interactions in Health and Disease,  Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

Human gut microbiome

A randomised, double- blind, cross-over study investigating the prebiotic effect of agave fructans in healthy human subjects – P. Ramnani – Journal of Nutritional Science

Water microbiome

Global dispersion and local diversification of the methane seep microbiome – S. Emil Ruff – PNAS USA

CRISPRs, phages, and viruses

Targeting Enterococcus faecalis biofilm using phage therapy – Leron Khalifa – Applied and Environmental Microbiology

Metagenomics

Quality control of microbiota metagenomics by k-mer analysis – Florian Plaza Onate – BMC Genomics

Expression of multiple horizontally acquired genes is a hallmark of both vertebrate and invertebrate genomes – Alastair Crisp – Genome Biology

Microbes in the news

Satire: Hipsterome – Pap Py – Megabiome

Satire: Hungry researchers have hard time replicating “Subway” microbiome study – Leaf E. Greens – Megabiome

Baby Microbiomes: Christi Investigates – Alexandra Carmichael – uBiome

Our viral companions – David Woodland – Summit Daily

Microbiome Editing with CRISPR/Cas9 Has Research, Diagnostic Potential – Andrew P. Han

Agave prebiotic significantly increases some faecal friendly bacteria, say researchers – Annie-Rose Harrison-Dunn – Nutraingredients

Horse dung has scientists on scent of antibiotic success – Jim Drury – Business Insider

Connection with bacteria found in sewage and weight gain – Tim Sandle – Digital Journal

Microbes and art

Gut Check: The Microbiome Game – David Coil – Microbe.net

“Gut Check is a game for 2-4 players where each player attempts to develop a healthy microbiome while interfering with the microbiomes of their opponents.”

Contaminant snowman – MicrobeWorld

Science, publishing, and career

Why you can’t always believe what you read in scientific journals – Julia Belluz – Vox

To Apply or Not to Apply: A Survey Analysis of Grant Writing Costs and Benefits – Ted von Hippel, Courtney von Hippel – PLOS ONE

Bik’s Picks

Your Eyes Are Wired Backwards: Here’s Why – Science 2.0

DNA is packaged like a yoyo, scientists find – Science Daily

Happy St. Patrick’s Day! Here’s the chemistry that makes your beer taste good (or bad) – Rachel Feltman – Washington Post

[hr]

March 15, 2015

Microbiome of pregnancy and birth

The vaginal microbiome during pregnancy and the postpartum period in a European population – David A. MacIntyre – Scientific Reports

Probiotic potential of Enterococcus faecalis strains isolated from meconium – Ahmed Khassaf Al Atyaa – Frontiers in Microbiology

Review: The human neonatal gut microbiome: a brief review – Emily C. Gritz and Vineet Bhandari – Frontiers in Pediatrics

Human gut microbiome

Gut microbiome of the Hadza hunter-gatherers – Stephanie L. Schnorr – Nature Communications

Gut microbiome variations during hematopoietic stem cell transplant in severe combined immunodeficiency – Jonathan P. Lane – Journal of Allergy and Clinical Immunology

Despite the title, this is 16S rRNA gene-based analysis: Metagenomic analysis of the impact of nitrofurantoin treatment on the human faecal microbiota – Jascha Vervoort – Journal of Antimicrobial Chemotherapy

Literature review: Mast Cell Activation Disease and Microbiotic Interactions – Lawrence B. Afrin, Alexander Khoruts- Clinical Therapeutics

Review: Autoimmune host–microbiota interactions at barrier sites and beyond – William E. Ruff, Martin A. Kriegel – Trends in Molecular Medicine

Review: Microbiota Regulation of the Mammalian Gut–Brain Axis – Aurelijus Buroka – Advances in Applied Microbiology

Review: IBD and the Gut Microbiota—from Bench to Personalized Medicine – Emanuelle Bellaguarda, Eugene B. Chang – Current Gastroenterology Reports

Review: Mutualistic presence of normal Flora in Human Intestine: A review – Karishma Kabir Nipa- Journal of Bio-Science

Animal models

Monosodium L-Glutamate and Dietary Fat Differently Modify the Composition of the Intestinal Microbiota in Growing Pigs – Feng Z-M – Obesity Facts

Altered profile of gut microbiota after subchronic exposure to neochamaejasmin A in rats – Lifeng Yan – Environmental Toxicology and Pharmacology

Effects of mulberry (Morus alba L.) leaf polysaccharides on growth performance, diarrhea, blood parameters, and gut microbiota of early-weanling pigs – Xiangjie Zhao – Livestock Science

Effect of grinding or pelleting high grain maize- or barley-based concentrates on rumen environment and microbiota of beef cattle – A. Gimeno – Animal Feed Science and Technology

Animal microbiome

Earthworms: Taxonomic and functional annotation of gut bacterial communities of Eisenia foetida and Perionyx excavatus – Arjun Singh – Microbiological Research

Plant microbiome

Diversity and community ecology of forest epiphyte testate amoebae from European Russia – Richard J. Payne – European Journal of Protistology

Soil and plant root microbiome

Effects of single-root-grafting, double-root-grafting and compost application on microbial properties of rhizosphere soils in Chinese protected cucumber (Cucumis sativus L.) production systems – Yinan Gao – Scientia Horticulturae

Response of belowground communities to short-term phosphorus addition in a phosphorus-limited woodland – Uffe N. Nielsen – Plant and Soil

Composition of soil microbiome along elevation gradients in southwestern highlands of Saudi Arabia – Muhammad Yasir – BMC Microbiology

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas – Chen Hong – Environmental Science and Pollution Research

Geomicrobiology of Iron Layers in the Sediment of Lake Superior – M. Dittrich – Aquatic Geochemistry

Water and ice microbiome

Microbial abundance in surface ice on the Greenland Ice Sheet – Marek Stibal – Frontiers in Microbiology

Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing – Chia Sing Chan – Frontiers in Microbiology

Review: New perspectives in benthic deep-sea microbial ecology – Cinzia Corinaldesi – Frontiers in Marine Science

Bioreactor microbiome

The microbial community of a passive biochemical reactor treating arsenic, zinc, and sulfate-rich seepage – Susan Anne Baldwin – Frontiers in Bioengineering and Biotechnology

Food microbiology

Process-driven bacterial community dynamics are key to cured meat colour formation by coagulase-negative staphylococci via nitrate reductase or nitric oxide synthase activities – María Sánchez Mainar, Frédéric Leroy – International Journal of Food Microbiology

Metagenomics and Bioinformatics

Modelling Computational Resources for Next Generation Sequencing Bioinformatics Analysis of 16S rRNA Samples – Matthew J. Wade – ArXiv

Accurate multiplexing and filtering for high-throughput amplicon-sequencing – Philippe Esling – Nucleic Acids Research

Transmission and Microevolution of USA300 MRSA in U.S. Households: Evidence from Whole-Genome Sequencing – Md Tauqeer Alam – mBio

Single cell genomics indicates horizontal gene transfer and viral infections in a deep subsurface Firmicutes population – Jessica Labonté – Frontiers in Microbiology

ISMapper: Identifying insertion sequences in bacterial genomes from short read sequence data – Jane Hawkey – BioRXiv

Perspective: Wiretapping into microbial interactions by single cell genomics – Ramunas Stepanauskas – Frontiers in Microbiology

Perspective: Bridging the knowledge gap: from microbiome composition to function – Jeremiah J Faith – Molecular Systems Biology

Metabolomics and Transcriptomics

Clostridium difficile heterogeneously impacts intestinal community architecture but drives stable metabolome responses – David Rojo – ISME Journal

Study of the diversity and short-chain fatty acids production by the bacterial community in overweight and obese Mexican children – S. Murugesan – European Journal of Clinical Microbiology & Infectious Diseases

Genomic Analysis of Xylose Metabolism in Members of the Deinoccocus-Thermus Phylum from Thermophilic Biomass-Deconstructing Bacterial Consortia – Yu-Wei Wu – BioEnergy Research

Proteomics as the final step in the functional metagenomics study of antimicrobial resistance – Fiona Fouhy – Frontiers in Microbiology

Fish: Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata) – Juan Jurado – Journal of Proteomics

Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals – Thuy Do – Frontiers in Cellular and Infection Microbiology

The transcriptional response of microbial communities in thawing Alaskan permafrost soils – M J. Coolen and William D. Orsi – Frontiers in Microbiology

Review: Mucin glycan foraging in the human gut microbiome – Louise Tailford – Frontiers in Genetics

More microbial science

Untangling the origin of viruses and their impact on cellular evolution – Arshan Nasir – Annals of the New York Academy of Sciences

The Great Escape: Pathogen Versus Host – Eric Kong, Mary Ann Jabra-Rizk – PLOS Pathogens

A fantastic voyage for sliding bacteria – Joshua D. Shrout – Trends in Microbiology

CRISPR-Cas Adaptive Immune Systems of the Sulfolobales: Unravelling Their Complexity and Diversity – Roger A. Garrett – Life

Etiology of Pericarditis in a prospective cohort of 1162 cases – Frédérique Gouriet – The American Journal of Medicine

Microbes in the news

Overselling the microbiome award: Dr. Raphael Kellman at MindBodyGreen – Jonathan Eisen – The Tree of Life
belongs to:
Why The Microbiome Is Your Key To Glowing Skin & Healthy Weight – Dr. Raphael Kellman – MindBodyGreen

The Era of the Microbiome – SXSW Session on Monday March 16

Mind Altering Microbes: How your gut microbiome may influence your mood – Jill Carnahan

CDC: Misuse of garments may have led to release of bacteria at Tulane lab – Debra Goldschmidt and Ed Payne – CNN

The dawn of the cyborg bacteria is here – Robert Boos – PRI

Famous Microbiologists – Paul Ehrlich – Today we honor Paul Ehrlich who was born on March 14 in 1912 – LumiByte

Science and art – microbial edition

Bacterial Paintings? New Art Uses Tiny Life Forms – Tia Ghose – LiveScience

How microbes inspire art (Video) – BBC

Fungi on Tea – Microbe World

Louis Pasteur T-shirt – The Strange company

Bik’s Picks

Five research papers that revolutionised health – Deborah Cohen – BBC News Health

Folic acid plus blood pressure drug lowers stroke risk: study – Daily Mail

Man Credits Hot Chili Sauce Challenge for Brain Cancer Diagnosis – Youth Health Magazine

[hr]

March 13, 2015

Pregnancy and birth

Comparative proteomics of human and macaque milk reveals species-specific nutrition during post-natal development – Kristen Lina Beck – Journal of Proteome Research

Human gut microbiome
Beta-diversity metrics of the upper digestive tract microbiome are associated with body mass index – Shih-Wen Lin – Obesity

Diversity of duodenal and rectal microbiota in biopsy tissues and luminal contents in healthy volunteers – Journal of Microbiology and Biotechnology

Perspective: Towards large-cohort comparative studies to define the factors influencing the gut microbial community structure of ASD patients – Daniel McDonald – Microbial Ecology in Health & Disease

Review: Gut microbiota: a key player in health and disease. A review focused on obesity
M. J. Villanueva-Millán – Journal of Physiology and Biochemistry

Review: Being human is a gut feeling – Thiago Hutter

Review: Hirschsprung’s Disease and the Intestinal Microbiome
Li Hong and Valeriy Poroyko

Animal and other models of microbiome research

Microbial communities and nutrient dynamics in experimental microcosms are altered after application of a high dose of Bti – Dagne Duguma – Journal of Applied Ecology

Animal microbiome

Review: Tales from the crypt and coral reef: the successes and challenges of identifying new herpesviruses using metagenomics – Charlotte J. Houldcroft and Judith Breuer – Frontiers in Microbiology

Soil and plant root microbiome

ReviewPlant-endophyte symbiosis, an ecological perspective – Zahoor Ahmed Wani – Applied Microbiology and Biotechnology

Niche partitioning in arbuscular mycorrhizal communities in temperate grasslands: a lesson from adjacent serpentine and non-serpentine habitats – Petr Kohout – Molecular Ecology

Effects of Forest Management Practices in Temperate Beech Forests on Bacterial and Fungal Communities Involved in Leaf Litter Degradation – Witoon Purahong – Microbial Ecology

Water microbiome

Antibiotics, Antibiotic Resistance Genes, and Bacterial Community Composition in Fresh Water Aquaculture Environment in China – Wenguang Xiong – Microbial Ecology

Grazer and viral impacts on microbial growth and mortality in the southern California Current Ecosystem – Alexis L. Pasulka – Journal of Plankton Research

Microstructure and cyanobacterial composition of microbial mats from the High Arctic – Asunción de los Ríos – Biodiversity and Conservation

Cyanobacterial diversity in benthic mats of the McMurdo Dry Valley lakes, Antarctica – L. Zhang – Polar Biology

Bioreactor microbiome
Responses of Microbial Communities to Single-Walled Carbon Nanotubes in Phenol Wastewater Treatment Systems – Yuanyuan Qu – Environmental Science & Technology

Microbiome of the built environment
Evolution of the indoor biome – NESCent Working Group on the Evolutionary Biology of the Built Environment – Trends in Ecology & Evolution

Metabolomics and proteomics

Metabolic Phenotyping of an Adoptive Transfer Mouse Model of Experimental Colitis and Impact of Dietary Fish Oil Intake – Francois-Pierre J. Martin – Journal of Proteome Research

Comparative proteomics of human and macaque milk reveals species-specific nutrition during post-natal development – Kristen Lina Beck – Journal of Proteome Research

Techniques

Flow cytometry of microbial communities: Facilitated gate setting by sequential dot plot scanning – Susanne Günther and Susann Müller – Cytometry Part A

Microbes in the news

Some genes ‘foreign’ in origin and not from our ancestors – Science Daily

MoBio Laboratories giving Research Package awards of microbiome analysis

Evotec, Second Genome To Collaborate In Microbiome Discovery And Development – Nasdaq

Why you shouldn’t be freaked out by the bacteria in public bathrooms – Megan Thielking – Vox

Bringing together science and design: Can changing how we wash change our skins microbiome? (2m video) – Experiment – Vimeo

Study: How microbiome affects liver’s response to medication – Samuel Million-Weaver – HS NewsBeat

New study may lead to microbiome-based colon cancer diagnostic – American Microbiome Institute

Nature’s inbuilt immune defense could protect industrial bacteria from viruses – Phys.org

Bacteria do the work at water reclamation plant – Lee Shearer – OnlineAthens

Paleo Cookbook Tells Moms to Swap Breast Milk for Bone Broth – Josh Scherer – TakePart

#BacteriaHysteria: NZ pre-schoolers carry potentially harmful bacteria – TVNZ

Science, publishing, and career

Why Science Fairs Aren’t So Fair – These K-12 events are hardly more than a competition among over-involved parents – Hana Schank – The Atlantic

Springer journal on hold for “pattern of inappropriate and compromised peer review” – Ivan Oransky – RetractionWatch

Bik’s Picks

American Pi: Why the Day Belongs to the U.S. (and Belize) – Philip Yam – Scientific American

How the Penguin Got Its Waddle – Ed Yong – New Yorker

How to make powdered booze at home – Paul Adams – Popular Science

[hr]