Microbiome digest, November 19, 2014

Skin microbiome of infants, viruses can replace bacteria for proper development of gut anatomy, and Amazon reviews of note.

Human skin microbiome

Evolving Concepts of Neonatal Skin – Carrie C. Coughlin and Alain Taïeb – Pediatric Dermatology

Clinical Approaches to Skin Cleansing of the Diaper Area: Practice and Challenges – Carrie C. Coughlin – Pediatric Dermatology

Psoriasis is not an autoimmune disease? – Lionel Fry – Experimental Dermatology

Animal models of microbiome research

* An enteric virus can replace the beneficial function of commensal bacteria – Elisabeth Kernbauer, Yi Ding & Ken Cadwell – Nature

Microbiology: A backup for bacteria – Yao Wang & Julie K. Pfeiffer – Nature

Animal microbiome

An Experimental Test of whether the Defensive Phenotype of an Aphid Facultative Symbiont Can Respond to Selection within a Host Lineage – Ailsa H.C. McLean – PLOS ONE

Effect of yeast with bacteriocin from rumen bacteria on laying performance, blood biochemistry, faecal microbiota and egg quality of laying hens – H. T. Wang – Journal of Animal Physiology and Animal Nutrition

* Symbiotic bacteria enable olive flies (Bactrocera oleae) to exploit intractable sources of nitrogen – M. Ben-Yosef – Journal of Evolutionary Biology

Plant microbiome

* Bacterial Endophytic Communities in the Grapevine Depend on Pest Management – Andrea Campisano – PLOS ONE

Metagenomics, transcriptomics

Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics – Zheng-Shuang Hua – ISME Journal

Community transcriptomics reveals unexpected high microbial diversity in acidophilic biofilm communities – Daniela S Aliaga Goltsman – ISME Journal

Ecology

Evolution of species interactions determines microbial community productivity in new environments – Francesca Fiegna – ISME Journal

Microbes in the news

Viruses as a Cure – Carl Zimmer – New York Times

A Lack of Bacteria Can Make You Overweight – Melinda Beck – The Wall Street Journal

Science, publishing and career

Stereotyping: PhD costume slammed – Nature

* She still needs help from Ken: Amazon reviews trash Barbie computer engineering book – Kevin Allen – Ragan’s PR Daily

Science and Art

This looks more like a genome than a plasmid, but it is pretty: ”Plasmid 29″ – Sandra Culliton – Etsy

Bik’s Picks

A hip-hop state of mind – Akeem Sule, Becky Inkster – The Lancet

 

House Passes Bill That Makes It Harder For Scientists To Advise The EPA – Emily Atkin – Think Progress

Mother’s soothing presence makes pain go away, changes gene activity in infant brain – Science Daily

Science (and Quacks) vs. the Aging Process – Tracey Samuelson – New York Times

Sorting Fact From Fiction and What the Best Science Writing Can Teach Us – Barbara J. King – Time

[hr]

Microbiome digest, November 18, 2014

Pregnancy and birth microbiome

Fetal exposures and perinatal influences on the stool microbiota of premature infants – Diana A. Chernikova – Journal of Maternal-Fetal and Neonatal Medicine

The Origin of Human Milk Bacteria: Is There a Bacterial Entero-Mammary Pathway during Late Pregnancy and Lactation? – Juan M. Rodríguez – Advances in Nutrition

Human oral microbiome

Dysbiosis and alterations in predicted functions of subgingival microbiome in chronic periodontitis – Mariana E. Kirst – Applied and Environmental Microbiology

Human respiratory microbiome

Analysis of Changes in Diversity and Abundance of the Microbial Community in a Cystic Fibrosis Patient Over a Multi-Year Period – Joshua R. Stokell – Journal of Clinical Microbiology

Human gut microbiome

* Gut Microbes and the Brain: Paradigm Shift in Neuroscience – Emeran A. Mayer – Journal of Neuroscience

Fiber supplementation influences phylogenetic structure and functional capacity of the human intestinal microbiome: follow-up of a randomized controlled trial – Hannah D Holscher – American Journal of Clinical Nutrition

Enteric microbiota leads to new therapeutic strategies for ulcerative colitis – Wei-Xu Chen – World Journal of Gastroenterology

Intestinal microbiota: The explosive mixture at the origin of inflammatory bowel disease? – Roberto Bringiotti – – World Journal of Gastroenterology

Direct Detection and Quantification of Bacterial Genes Associated with Inflammation in DNA Isolated from Stool – Ramon Gomez-Moreno – Advances in Microbiology

Review: The gut microbiome in health and in disease – Shreiner, Andrew B – Current Opinion in Gastroenterology

Animal models of microbiome research

* Composition of Intestinal Microbiota in Immune-Deficient Mice Kept in Three Different Housing Conditions – Christa Thoene-Reineke – PLOS ONE

The Gut Microbiota Modulates Glycaemic Control and Serum Metabolite Profiles in Non-Obese Diabetic Mice – Thomas U. Greiner – PLOS ONE

Animal microbiome

* Convergent Bacterial Microbiotas in the Fungal Agricultural Systems of Insects – Frank O. Aylward – mBio

* Optimization of preservation and processing of sea anemones for microbial community analysis using molecular tools – Joana Rocha – Scientific Reports

Global discovery of colonization determinants in the squid symbiont Vibrio fischeri – John F. Brooks, II – PNAS US

Plant microbiome

It takes an individual plant to raise a community: TRFLP analysis of the rhizosphere microbial community of two pairs of high- and low-metal-accumulating plants – Melanie P. Columbus – Soil Biology and Biochemistry

Soil and sediment microbiome

* Uncoupling of microbial community structure and function in decomposing litter across beech forest ecosystems in Central Europe – Witoon Purahong – Scientific Reports

Different Continuous Cropping Spans Significantly Affect Microbial Community Membership and Structure in a Vanilla-Grown Soil as Revealed by Deep Pyrosequencing – Wu Xiong – Microbial Ecology

Deforestation and cultivation with Maize (Zea mays L.) has a profound effect on the bacterial community structure in soil – Elizabeth Selene Gómez-Acata – Land Degradation & Development

Soil pH Determines Microbial Diversity and Composition in the Park Grass Experiment – Kateryna Zhalnina – Microbial Ecology

Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River – Anna Freixa – Fundamental and Applied Limnology / Archiv für Hydrobiologie

Salt effects on the soil microbial decomposer community and their role in organic carbon cycling: A review – Kristin M. Rath – Soil Biology and Biochemistry

Water microbiome

* Metagenome Sequencing of the Microbial Community of a Solar Saltern Crystallizer Pond at Cáhuil Lagoon, Chile – Alvaro M. Plominsky – Genome Announcements

Experimental evidence for strong stabilizing forces at high functional diversity of aquatic microbial communities – Francesco Carrara – ESA Ecology

Microbiome of the built environment

* Ecological succession and viability of human-associated microbiota on restroom surfaces – Sean M. Gibbons – Applied and Environmental Microbiology

* Seasonal Variability in Airborne Biotic Contaminants in Swine Confinement Buildings – Priyanka Kumari – PLOS ONE

Metabolomics

Bacterial microcompartments and the modular construction of microbial metabolism – Cheryl A. Kerfeld – Trends in Microbiology

Suppression of Microbial Metabolic Pathways Inhibits the Generation of the Human Body Odor Component Diacetyl by Staphylococcus spp – Takeshi Hara – PLOS ONE

Bioinformatics

A phylogeny-based sampling strategy and power calculator informs genome-wide
associations studydesign for microbial pathogens – Maha R. Farhat – Genome Medicine

* Poster: Assessment of eukaryotic communities in environmental samples: A workflow comparison for next-generation sequencing data – Kristin Hardge – ePIC

Archaea

Phylogenetically Driven Sequencing of Extremely Halophilic Archaea Reveals Strategies for Static and Dynamic Osmo-response – Erin A. Becker – PLOS Genetics

More microbiology papers

The commonly used antimicrobial additive triclosan is a liver tumor promoter – Mei-Fei Yueh – PNAS USA

Microbes in the News

Virus linked to mass die-off of sea stars – Craig Welch – The Seattle Times

Bik’s Picks

Wild chimpanzees plan their breakfast time, type, and location – Karline R. L. Janmaat – PNAS USA

Here Are 4 More Vulnerable Fish to Avoid Next Time You’re at the Sushi Bar – Dan Kedmey – Time

Comet landing: Organic molecules detected by Philae – Paul Rincon – BBC News

Correlation instead of causation?: Coffee Creamer, Crackers Linked To Worse Memory – Science2.0

[hr]

Microbiome digest, November 13, 2014

Microbiome of pregnancy and birth

Hyaluronan in cervical epithelia protects against infection-mediated preterm birth – Yucel Akgul – Journal of Clinical Investigation

The Impact of the Milk Glycobiome on the Neonate Gut Microbiota – Alline R. Pacheco – Annual Review of Animal Biosciences

Human oral microbiome

The oral microbiome and the immunobiology of periodontal disease and caries – Massimo Costalonga – Immunology Letters

Human gut microbiome

Intestinal microbiota as modulators of the immune system and neuro-immune system: impact on the host health and homeostasis – Carlos Magno da Costa Maranduba – Journal of Immunology Research

The role of the microbiota in inflammation, carcinogenesis, and cancer therapy – Amiran Dzutsev – European Journal of Immunology

Animal microbiome

The Insect Microbiome Modulates Vector Competence for Arboviruses – Natapong Jupatanakul – Viruses

Bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing – Prakaimuk Saraithong – Insect Science

Pezizomycotina dominates the fungal communities of South China Sea Sponges Theonella swinhoei and Xestospongia testudinaria – Liling Jin – FEMS Microbiology Ecology

Plant microbiome

Plant lignin content altered by soil microbial community – Alison E. Bennett – New Phytologist

Bioreactor microbiome

Ciprofloxacin Residues in Municipal Biosolid Compost Do Not Selectively Enrich Populations of Resistant Bacteria – Caitlin P. Youngquist – Applied and Environmental Microbiology

Food microbiology

Spatial Distribution of Bacterial Communities and Related Biochemical Properties in Luzhou-Flavor Liquor-Fermented Grains – Jia Zheng- Journal of Food Science

Bacterial Community Dynamics of Salted and Fermented Shrimp based on Denaturing Gradient Gel Electrophoresis – Kook-Il Han – Journal of Food Science

Metabolomics

Microbiota-dependent metabolite trimethylamine-N-oxide is associated with disease severity and survival of patients with chronic heart failure – Marius Trøseid – Journal of Internal Medicine

Techniques

* Performance Comparison of Illumina and Ion Torrent Next-Generation Sequencing Platforms for 16S rRNA-Based Bacterial Community Profiling – Stephen J. Salipante – Applied and Environmental Microbiome

Impact of T-RFLP data analysis choices on assessments of microbial community structure and dynamics – Nils Johan Fredriksson – BMC Bioinformatics

A Hidden Pitfall in the Preparation of Agar Media Undermines Microorganism Cultivability – Tomohiro Tanaka – Applied and Environmental Microbiome

Microbes in the news

Contamination plagues some microbiome studies – Elizabeth Pennisi – Science Magazine

37 Trillion Invaders: We Are Not Alone – Science 2.0

Spilling Our Guts: Decreased Diversity in the Human Microbiome (podcast) – ScienceFriday

Nature, as Well as Nurture, Shapes Human Gut Microbiome – Lara C. Pullen – Medscape

Enterome Enters into a Microbiome-Based Development Collaboration with AbbVie in Crohn’s Disease – PRNewsWire

Pillow germs: Testing revealed bacteria growing on bed pillows; how often you should replace them – KJRH

“The Lung Ranger” Cystic Fibrosis Device From Dundee University Wins 3 Awards – Anna Tan – CysticFibrosisNewsToday

Science and Career

The point of it all – Joseph Swift – Science Magazine

No sexism in science? Not so fast, critics say – Rachel Bernstein – Science Magazine

[hr]

Microbiome digest, November 7, 2014

General microbiome

Special issue of Cell Metabolism on Microbiome:

The Multifaceted Metabolic Microbiome—Marching toward Function – Nikla Emambokus – Cell Metabolism
http://www.sciencedirect.com/science/article/pii/S1550413114004653

A Bitter Aftertaste: Unintended Effects of Artificial Sweeteners on the Gut Microbiome – Nicholas A. Bokulich, Martin J. Blaser
http://www.sciencedirect.com/science/article/pii/S1550413114004598

Specialized Metabolites from the Microbiome in Health and Disease – Gil Sharon
http://www.sciencedirect.com/science/article/pii/S155041311400463X

Determining Microbial Products and Identifying Molecular Targets in the Human Microbiome – Regina Joice
http://www.sciencedirect.com/science/article/pii/S1550413114004501

Vitamin B12 as a Modulator of Gut Microbial Ecology – Patrick H. Degnan
http://www.sciencedirect.com/science/article/pii/S1550413114004495

γ-Butyrobetaine Is a Proatherogenic Intermediate in Gut Microbial Metabolism of L-Carnitine to TMAO – Robert A. Koeth
http://www.sciencedirect.com/science/article/pii/S1550413114004537

Human vaginal microbiome

Interplay Between the Temporal Dynamics of the Vaginal Microbiota and Human Papillomavirus Detection – Rebecca M. Brotman – Journal of Infectious Diseases
http://jid.oxfordjournals.org/content/210/11/1723.abstract

A novel role for maternal stress and microbial transmission in early life programming and neurodevelopment – Eldin Jašarević – Neurobiology of Stress
http://www.sciencedirect.com/science/article/pii/S2352289514000137

Human gut microbiome

Human Genetics Shape the Gut Microbiome – Julia K. Goodrich – Cell
http://www.sciencedirect.com/science/article/pii/S0092867414012410

The potential beneficial role of faecal microbiota transplantation in diseases other than Clostridium difficile infection – R. Singh – Clinical Microbiology and Infection
http://onlinelibrary.wiley.com/doi/10.1111/1469-0691.12799/abstract

Gastrointestinal microbiota in children with autism in Slovakia – Aleksandra Tomova – Physiology & Behavior
http://www.sciencedirect.com/science/article/pii/S0031938414005101

In vitro models

Comparative study of the in vitro fermentative characteristics of fenugreek gum, white bread and bread with fenugreek gum using Human faecal microbes – K.T. Roberts – Bioactive Carbohydrates and Dietary Fibre
http://www.sciencedirect.com/science/article/pii/S2212619814000515

Animal microbiome

The draft genome, transcriptome, and microbiome of Dermatophagoides farinae reveal a broad spectrum of dust mite allergens – Ting-Fung Chan – Journal of Allergy and Clinical Immunology
http://www.sciencedirect.com/science/article/pii/S0091674914013669

Soil microbiome

(not really microbiome, but of interest):

Pedodiversity – Rare earth – Michael Tennesen – Science
http://www.sciencemag.org/content/346/6210/692.summary

Water microbiome

Viral attack exacerbates the susceptibility of a bloom-forming alga to ocean acidification – Shanwen Chen – Global Change Biology
http://onlinelibrary.wiley.com/doi/10.1111/gcb.12753/abstract

Food microbiology

Microbiota and metabolites of aged bottled gueuze beers converge to the same composition
Freek Spitaels – Food Microbiology
http://www.sciencedirect.com/science/article/pii/S0740002014002548

Microbiological changes, shelf life and identification of initial and spoilage microbiota of sea bream fillets stored under various conditions using 16S rRNA gene analysis – Foteini F Parlapani – Journal of the Science of Food and Agriculture
http://onlinelibrary.wiley.com/doi/10.1002/jsfa.6957/abstract

More microbiology

The Evolution and Genetics of Virus Host Shifts – Ben Longdon – PLOS Pathogens
http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004395

Microbes in the news

When obesity is an inherited trait, maybe gut bacteria is the link – Melissa Healy – LA Times
http://www.latimes.com/science/sciencenow/la-sci-sn-obesity-genes-gut-bacteria-20141106-story.html

Norovirus: The Perfect Pathogen Emerges From the Shadows – Carl Zimmer – National Geographic
http://phenomena.nationalgeographic.com/2014/11/06/norovirus-the-perfect-pathogen-emerges-from-the-shadows/

Science, publishing and career

Make data sharing easy: PLOS launches its Data Repository Integration Partner Program – Jennifer Lin – PLOS Blogs

Make data sharing easy: PLOS launches its Data Repository Integration Partner Program

Satire: Manuscript Becomes Highly-Cited Without PR Campaign – The Allium
http://www.theallium.com/science-life/manuscript-becomes-highly-cited-without-pr-campaign/

Bik’s Picks

Origin of the unique ventilatory apparatus of turtles: How the tortoise’s ribs got embedded in its shell – Science Daily
http://www.sciencedaily.com/releases/2014/11/141107111042.htm

Killer Chairs: How Desk Jobs Ruin Your Health – James Levine – Scientific American
http://www.scientificamerican.com/article/killer-chairs-how-desk-jobs-ruin-your-health/

Allergic to Penicillin? You’re Probably Not – Judy Silverman and Maggie Fox – NBC News
http://www.nbcnews.com/health/health-news/allergic-penicillin-youre-probably-not-n243161

[hr]

Microbiome Digest, November 4, 2014

Tomorrow, Wednesday November 5, at 1:00pm USA-EST : Upcoming AMA (Ask Me Anything) in reddit/r/science: “Microbiota of the human gut” with my co-worker Les Dethlefsen. Ask him anything! You can submit questions, vote for the best questions, and the top questions will be answered by Les.

Human gut microbiome

* Rapid changes in the gut microbiome during human evolution – Andrew H. Moeller – PNAS USA

“To establish how the gut microbiome has changed since the diversification of human and ape species, we characterized the microbial assemblages residing within hundreds of wild chimpanzees, bonobos, and gorillas.”

and: Human Gut Bacteria Much Different Than Apes – Science 2.0

* Blastocystis Is Associated with Decrease of Fecal Microbiota Protective Bacteria: Comparative Analysis between Patients with Irritable Bowel Syndrome and Control Subjects – Céline Nourrisson – PLOS ONE

“Here, we first compared the prevalence of Blastocystis among 56 IBS patients (40 IBS with constipation (IBS-C), 9 IBS with diarrhea (IBS-D), 4 mixed IBS (IBS-M) and 3 unsubtyped IBS (IBS-U) according to the Rome III criteria) and 56 control (i.e. without any diagnosed chronic or acute gastrointestinal disorder) subjects. “

Animal models of microbiome research

* Bone Marrow Dendritic Cells from Mice with an Altered Microbiota Provide Interleukin 17A-Dependent Protection against Entamoeba histolytica Colitis – Stacey L. Burgess – mBio

“In studies utilizing a murine model, we demonstrated that colonization of the gut with the commensal Clostridia-related bacteria known as segmented filamentous bacteria (SFB) is protective during E. histolytica infection.”

Animal microbiome

Effect of Copper Treatment on the Composition and Function of the Bacterial Community in the Sponge Haliclona cymaeformis – Ren-Mao Tian – mBio

“16S rRNA gene sequencing results showed that the sponge Haliclona cymaeformis harbored symbiotic sulfur-oxidizing Ectothiorhodospiraceae and photosynthetic Cyanobacteria as dominant species. “

Bioinformatics tools

Massive fungal biodiversity data re-annotation with multi-level clustering – Duong Vu – Scientific Reports

“An implementation of the algorithm allowed clustering of all 344,239 ITS (Internal Transcribed Spacer) fungal sequences from GenBank”

Viruses and phages

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applied to virus identification – Adriana Calderaro – Scientific Reports

“The results revealed MALDI-TOF MS to be an effective and inexpensive tool for the identification of the three poliovirus serotypes. “

More microbiology

Occurrence and expression of bacterial human virulence gene homologues in natural soil bacteria – Ditte A. Søborg – FEMS Microbial Ecology

“About 25% of the bacterial isolates contained virulence gene homologues representing toxin (hblA, cytK2), adhesin (fimH), regulator (phoQ) and resistance (yfbI) determinants in pathogenic bacteria.”

Science, publishing, and career

* Don’t Worry Your Pretty Little Heads – Rebecca Schuman – Slate

“I don’t understand how they wrote the paper or the op-ed they did while looking at the same results I see in their paper. … Where I come from,” she concludes, “we call that institutional bias.”

Bik’s Picks

Birds found using human musical scales for the first time – Virginia Morell – Science Blog

“The flutelike songs of the male hermit thrush (Catharus guttatus) are some of the most beautiful in the animal kingdom.”

Vaccine-resistant polio strain discovered – Science Daily

“The DNA sequence shows two mutations, unknown until now, of the proteins that form the “shell” (capsid) of the virus.”

The appliance of science: home experiments with your kids – Louise Holden – Irish Times

“Next week is Science Week, and one of the messages that McCarthy is hoping to put out there is that science is for everybody, not just those hoping to work in the sector. Helping your child to become science-literate is a great gift.”

The top 20 catchiest songs of all time, according to science – Michelle Starr – CNET

“A year-long survey by the Museum of Science and Industry in Manchester has revealed the top 10 most persistent earworms — with the Spice Girls topping the list.”

[hr]

Microbiome digest, October 30, 2014

Bacteriocins in human metagenomic libraries, freezing/thawing effects on community composition, microbiome of preterm neonates, and bacteria in soil.
Multiple-site human microbiome

Diversity and dynamics of bacteriocins from human microbiome – Jinshui Zheng – Environmental Microbiology

“We employed bioinformatic analyses to identify putative bacteriocin sequences in metagenomic sequences obtained from different human body sites. “

Human respiratory microbiome

* Implications of multiple freeze-thawing on respiratory samples for culture-independent analyses – Leah Cuthbertson – Journal of Cystic Fibrosis

“Following each cycle, an aliquot was removed and treated with propidium monoazide (PMA) prior to DNA extraction and 16S rRNA gene pyrosequencing.”

Human gut microbiome

Immune Homeostasis, Dysbiosis and Therapeutic Modulation of the Gut Microbiota – Christine T. Peterson – Clinical & Experimental Immunology

“This review discusses host-microbiota homeostasis, consequences of its perturbation, and the associated challenges in therapeutic developments that lie ahead.”

Microbiome of pregnancy and birth

* Intestinal Microbiota Development in Preterm Neonates and Effect of Perinatal Antibiotics – Silvia Arboleya – The Journal of Pediatrics

“We used 16S ribosomal RNA gene sequence-based microbiota analysis and quantitative polymerase chain reaction to evaluate the establishment of the intestinal microbiota. “

Animal microbiome

Hidden Population Structure and Cross-species Transmission of Whipworms (Trichuris sp.) in Humans and Non-human Primates in Uganda – Ria R. Ghai – PLOS Neglected Tropical Diseases

“We examined the phylogenetic structure of whipworms in a multi-species community of non-human primates and humans in Western Uganda, using both traditional microscopy and molecular methods.”

Soil microbiome

Metagenomic analysis reveals microbial functional redundancies and specificities in a soil under different tillage and crop-management regimes – Renata Carolini Souza – Applied Soil Ecology

“in this study we used a shotgun metagenomic approach to compare different soil…, and crop … managements in a 13-year-old field experiment in southern Brazil. “

Evaluating T-RFLP protocols to sensitively analyze the genetic diversity and community changes of soil alkane degrading bacteria – Julia Giebler – European Journal of Soil Biology

“This study aimed at identifying a reliable, highly resolving fingerprinting method to monitor alkane degrading communities in soil.”

Contrasting land uses in Mediterranean agro-silvo-pastoral systems generated patchy diversity patterns of vascular plants and below-ground microorganisms – Simonetta Bagella – Comptes Rendus Biologies

“Species richness decreased with increasing land use intensity, the Shannon index showed the highest values in grasslands and hay crops.”

Food microbiology

Characterization and use of microbial communities in Doenjang to control the unpleasant odor of Ginkgo epicarp – Sunhyun Park – Food Science and Biotechnology

“Microbes in doenjang were probably responsible for the reduction in the butyric acid content. Microbial communities were analyzed during fermentation.”

Bioreactor microbiology

Thermophilic anaerobic digestion of thermal pretreated sludge: role of microbial community structure and correlation with process performances – M.C. Gagliano – Water Research

“16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. “

Bioinformatics

Fast dendrogram-based OTU clustering using sequence embedding – Thuy-Diem Nguyen – Proceedings of the 5th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics

“In this paper, we introduce a new hierarchical clustering method called CRiSPy-Embed which aims to produce high-quality clustering results at a low computational cost. “

Focus: a new multilayer graph model for short read analysis and extraction of biologically relevant features – Julia Warnke – Proceedings of the 5th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics

“We present Focus, a new multilayer graph model for short read analysis and extraction of biologically relevant features. “

The UniFrac significance test generates different outputs given semantically equivalent inputs – Jeffrey Long – Proceedings of the 5th ACM Conference on Bioinformatics, Computational Biology, and Health Informatics

“We identify a discrepancy in the UniFrac methodology that causes semantically equivalent inputs to produce different outputs in tests of statistical significance.”

Microbes in the news

SpaceX 4 splashes down, returns bacteria samples to Earth – Josh Barrett – WaayTV

“HudsonAlpha’s Genomic Sciences Lab will be sequencing the genomes of E. coli bacteria that were aboard the ISS. For some reason, bacteria are not as susceptible to antibiotics in space.”

Bik’s Picks

Why Millennials &%#@! Love Science – Alexandra Ossola – The Atlantic

“Today’s young adults see new discoveries both as a source of awe and a means for innovation.”

1,000 Flavors of Banana: the New Science of Food Additives – Annie Gasparro – The Wall Street Journal

“Rather than sourcing the jalapeño or poblano pepper, you can create it with a flavor house, and just apply it over a corn chip,” said Jared Simon, marketing director for Hain Celestial Group Inc. ’s snacks and bakery division, which uses natural flavors for its foods such as Terra vegetable chips.”

Spooky Science: The Sounds of Halloween – Science Buddies – Scientific American

“In this science activity, you will investigate what goes into the composition of a perfect creepy soundtrack. “

[hr]

Non-human microbiome digest, October 27, 2014

Microbiomes of camels, cheetahs, jackals, birds and reptiles, limpets, corals, plant roots, soils, and sea water.

Animal microbiome

* In-depth diversity analysis of the bacterial community resident in the camel rumen – Javad Gharechahi – Systematic and Applied Microbiology

“In this study, 16S rRNA gene amplicon pyrosequencing was applied in order to explore the structure of the bacterial community inhabiting the camel rumen.”

Oligotyping reveals differences between gut microbiomes of free-ranging sympatric Namibian carnivores (Acinonyx jubatus, Canis mesomelas) on a bacterial species-like level – Sebastian Menke – Frontiers in Microbiology

“Here, we used Illumina MiSeq to sequence amplicons generated from the V4 region of the 16S rRNA gene to investigate the gut microbiome of two free-ranging sympatric Namibian carnivore species, the cheetah (Acinonyx jubatus) and the black-backed jackal (Canis mesomelas).”

Diet is a major factor governing the fecal butyrate-producing community structure across Mammalia, Aves and Reptilia – Marius Vital – ISME Journal

“We investigated the butyrate-producing community in hindgut-fermenting Mammalia (n=38), Aves (n=8) and Reptilia (n=8) using a gene-targeted pyrosequencing approach of the terminal genes of the main butyrate-synthesis pathways”

Decadal changes in common reef coral populations and their associations with algal symbionts (Symbiodinium spp.) – Timothy R. McClanahan – Marine Ecology

“Decadal populations changes in four coral taxa and their patterns of association with algal symbionts (Symbiodinium spp. – 10 years of sampling) were examined on Kenyan back reefs over a period of climatic disturbances (1991−2009). “

Metaphylogenomic and Potential Functionality of the Limpet Patella pellucida’s Gastrointestinal Tract Microbiome – Magda Dudek – International Journal of Molecular Sciences

“This study investigated the microbial diversity associated with the digestive tract of the seaweed grazing marine limpet Patella pellucida.”

Plant microbiome

* Distinct root-associated bacterial communities on three wild plant species growing in a common field – Kristin Aleklett – PeerJ Preprints

“We sampled endophytic and epiphytic bacteria in root tissues from a population of a wild, clonal plant… as well as two neighboring plant species … to determine if plant species hosted unique root microbiota”

The fungal leaf endophyte Paraconiothyrium variabile specifically metabolizes the host-plant metabolome for its own benefit – Yuan Tian – Phytochemistry

“..we have investigated the action of the endophytic fungus Paraconiothyrium variabile, isolated from the leaves of Cephalotaxus harringtonia, on the secondary metabolome of the host-plant. “

Soil microbiome

Late winter under ice pelagic microbial communities in the high Arctic Ocean and the impact of short-term exposure to elevated CO2 levels – Adam Monier – Frontiers in Microbiology

“Phylogenetic diversity was greater in the attached fraction compared to the free-living fraction in situ, in the controls and in the treatments.”

The ecological dichotomy of ammonia-oxidizing archaea and bacteria in the hyper-arid soils of the Antarctic Dry Valleys – Catarina M. Magalhães – Frontiers in Microbiology

“Here we showed that AOB and AOA amoA gene diversity was generally low; only four AOA and three AOB operational taxonomic units (OTUs) were identified from a total of 420 AOA and AOB amoA clones.”

Soil bacterial community composition altered by increased nutrient availability in Arctic tundra soils – Akihiro Koyama – Frontiers in Microbiology

“Fertilization significantly altered overall bacterial community composition and reduced evenness, to a greater degree in organic than mineral soils, and in the 1989 compared to the 2006 site.”

Effect of volatile organic compounds absorbed to fresh biochar on survival of Bacillus mucilaginosus and structure of soil microbial communities – Daquan Sun – Journal of Soils and Sediments

“The structure of a soil microbial community incubated in biochar was examined via denaturing gradient gel electrophoresis (DGGE). “

Broad host range plasmids can invade an unexpectedly diverse fraction of a soil bacterial community – Uli Klümper – ISME Journal

“We identified transfer to many different recipients belonging to 11 different bacterial phyla. “

Water microbiome

Genomes and gene expression across light and productivity gradients in eastern subtropical Pacific microbial communities – Chris L Dupont – ISME Journal

“Relative to surface waters, bacterial SCM communities displayed greater genetic diversity and enrichment in putative sulfur oxidizers, multiple actinomycetes, low-light-adapted Prochlorococcus and cell-associated viruses.”

Aerobic methanotrophic communities at the Red Sea brine-seawater interface – Rehab Z. Abdallah – Frontiers in Microbiology

“The current study analyzed the aerobic free–living methane-oxidizing bacterial communities that potentially contribute to methane oxidation at the brine-seawater interfaces of the three aforementioned brine pools, using metagenomic pyrosequencing, 16S rRNA pyrotags and pmoA library constructs. “

Bacterial community structure is indicative of chemical inputs in the Upper Mississippi River – Christopher Staley – Frontiers in Microbiology

“Bacterial communities were characterized by Illumina sequencing of the V6 region of 16S rDNA and compared to >40 chemical and nutrient concentrations. “

Semiconductor Sequencing Reveals the Diversity of Bacterial Communities in an Amazonian Reservoir – Diego Assis das Graças – Aquatic Science and Technology

“In this study, we used a next-generation sequencing platform, the Ion Torrent Personal Genome Machine, and employed a barcode library to investigate the bacterial diversity along a 70-m deep water column in the proximity of the dam. “

Seasonal changes of microbial communities in two shallow peat bog lakes – Sylwia Lew – Folia Microbiologica

“We investigated the seasonal dynamics of the microbial community in two small peat bog lakes, Kuźnik Olsowy and Kuźnik Bagienny, located in western Poland.”

Bioreactor microbiome

Methane-fed microbial microcosms show differential community dynamics and pinpoint taxa involved in communal response – Igor Y Oshkin – ISME Journal

“We report observations on the dynamics of bacterial communities in response to methane stimulus in laboratory microcosm incubations prepared with lake sediment samples.”

[hr]

Human microbiome digest, October 27, 2014

Development of gut microbiome in infants, understanding oral microbiome through high-throughput sequencing, gut bacteria in deceased alcoholics, and bacteria after stroke.

Microbiome general

The symbiont side of symbiosis: do microbes really benefit? – Justine R. Garcia and Nicole M. Gerardo – Frontiers in Microbiology

“We evaluate these hypotheses based on available evidence, which indicate reduced competition and predation are not universal benefits for symbionts. “

Pregnancy and birth microbiome

Review: Diet and the development of the human intestinal microbiome – Noah Voreades – Frontiers in Microbiology

“This review focuses primarily on diet, as it is one of the most pivotal factors in the development of the human gut microbiome from infancy to the elderly.”

Human oral microbiome

Review: Application of high-throughput sequencing in understanding human oral microbiome related with health and disease – Hui Chen and Wen Jiang – Frontiers in Microbiology

“This review describes the application of high-throughput sequencing for characterization of oral microbiota and analyzing the changes of the microbiome in the states of health or disease.”

Human gut microbiome

Cellular and molecular pathways through which commensal bacteria modulate sensitization to dietary antigens – Taylor Feehley, Cathryn R Nagler – Current Opinion in Immunology

“This review will discuss the cellular and molecular pathways activated by commensal bacteria to protect against allergic sensitization.”

Microbial Therapy in Liver Disease: Probiotics Probe the Microbiome–Gut–Liver–Brain Axis – David W. Victor 3rd, Eamonn M.M. Quigley – Gastroenterology

“It is timely, therefore, that Dhiman et al should embark on an ambitious study, reported in this issue of Gastroenterology, designed to assess the efficacy of the probiotic cocktail, VSL#3, as secondary prophylaxis in patients with OHE.”

Changes in gut bacterial populations and their translocation into liver and ascites in alcoholic liver cirrhotics – Sari Tuomisto – BMC Gastroenterology

“Relative ratios of major commensal bacterial communities (Bacteroides spp., Bifidobacterium spp., Clostridium leptum group, Enterobactericaea and Lactobacillus spp.) were determined in faecal samples from post mortem examinations performed on 42 males”

Animal models of microbiome research

Age-related changes in intestinal permeability and gut microbiota after ischemic stroke – Joshua Crapser – Journal of Neuroimmunology

“This study aimed to elucidate the role of systemic factors, such as intestinal stress, underlying the poor outcomes of aging mice after stroke.”

Spatial and temporal colonization dynamics of segmented filamentous bacteria is influenced by gender, age and experimental infection with Helicobacter hepaticus in Swiss Webster mice – Zhongming Ge – Microbes and Infection

“Hh infection in both genders did not alter SFB levels in the jejunum and ileum, but increased SFB levels in the cecum and colon of males compared to the controls”

[hr]

Microbiome digest, October 24, 2014

Microbiome of placentas, infant respiratory microbiome, metabolomics, mutipartite genomes, microbes in the news, and some weekend picks.
Human microbiome general

Microbiome/microbiota and allergies – Yuzaburo Inoue, Naoki Shimojo – Seminars in Immunopathology

“In this review, we summarize the recent findings regarding the importance of the microbiome/microbiota in the development of allergic diseases and also the results of interventional studies using probiotics or prebiotics to prevent allergies.”

Pregnancy and birth

Term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery – Ronan M. Doyle – Placenta

“In this study, 16S rDNA pyrosequencing was used to identify bacteria in placental membranes. Caesarean sections and vaginal deliveries at term were found to harbour common genera”

Early Respiratory Microbiota Composition Determines Bacterial Succession Patterns and Respiratory Health in Children – Giske Biesbroek – Am J Respir Crit Care Med.

“Upper respiratory microbiota profiles of 60 healthy children at the ages of 1.5, 6, 12 and 24 months were characterized by 16S-based pyrosequencing”

From the “old NEC” to the “new NECs” – Melania Puddu – Journal of Pediatric and Neonatal Individualized Medicine

“This review describes in detail the new NECs especially those which affect preterm infants: contagion or lymphocytosis associated, transfusion associated and cow’s milk allergy associated.”

Metagenomics

The effects of variable sample biomass on comparative metagenomics – Meghan Chafee – Environmental Microbiology

“We investigated the interacting effects of DNA input and library amplification by PCR on comparative metagenomic analysis using dilutions of a single complex template from an Arabidopsis-associated microbial community.”

Metabolomics 

The nexus of syntrophy-associated microbiota in anaerobic digestion revealed by long-term enrichment and community survey – Takashi Narihiro – Environmental Microbiology

“16S pyrotag analysis revealed core populations of known syntrophs (six clades) and methanogens (nine clades) associated with acid degradation, and evidence for substrate- and/or inoculum-dependent specificity in syntrophic partnerships.”

In Vitro Fermentation of Lactulose by Human Gut Bacteria – Bingyong Mao – Journal of Agricultural and Food Chemistry

“This study aimed to identify lactulose-metabolizing bacteria in the human gut, using in-silico and traditional culture methods. “

Epigenomic regulation of host–microbiota interactions – Theresa Alenghat, David Artis – Trends in Immunology

“We review recent advances in understanding the interplay between the microbiota and mammalian epigenomic pathways, and highlight emerging findings that implicate a central role for histone deacetylases (HDACs) in orchestrating host–microbiota interactions.”

More microbiology

Examination of Prokaryotic Multipartite Genome Evolution through Experimental Genome Reduction – George C. diCenzo – PLOS Genetics

“To gain insight into the role and evolutionary history of these replicons, we have ‘reversed evolution’ by constructing a S. meliloti strain consisting solely of the chromosome and lacking the pSymB chromid and pSymA megaplasmid.”

Microbes in the news

How An Antibiotic Gene Jumped All Over The Tree of Life – Ed Yong – National Geographic

“Metcalf showed that it has also jumped from bacteria into every other major branch of life. It’s in animals, plants, fungi, archaea, and even some viruses.”

Research investigates bacteria on banknotes – Harper Adams University

“This study investigates how easy it is for bacteria and pathogens to survive on currency and whether the material the notes are made of, has an effect on the transfer.”

How millennials will save us all – Jennifer Block – Quartz

“Millennials get a bad rap for loading up on debt, mooching off their parents, and hiding behind technology. But there’s one problem that they may be particularly suited to tackling: our shrinking microbiome.“

Bik’s Picks

World’s Most Asked Questions: How Much Water Should I Drink a Day? – SciShow – Youtube

“People ask Google everything under the sun. One of the most commonly searched questions in the world is “How much water should I drink a day?” SciShow has the answer!”

Ten Simple Rules for Writing a PLOS Ten Simple Rules Article – Harriet Dashnow – PLOS Computational Biology

“This search left us wanting, until we discovered the PLOS Ten Simple Rules collection. We have found them to be a series of concise articles that capture the professional zeitgeist of being a scientist in an approachable manner.”

Satire: Ambitious New High-Speed Rail Plan Will Fly Americans To Japan To Use Their Trains – The Onion

“The transit system that the U.S. has needed for so long is now just a 7,000-mile plane journey to Tokyo’s Narita International Airport and a brief passage through Japanese customs away,” Obama continued.”

Microbiome Digest, October 23, 2014

Not much microbiome news today – which was good because I got time to watch the solar eclipse and a huge solar storm at the same time.  Enjoy the read!

Animal models of microbiome research

Lyn Deficiency Leads to Increased Microbiota-Dependent Intestinal Inflammation and Susceptibility to Enteric Pathogens – Morgan E. Roberts – Journal of Immunology

“Lyn2/2 mice exhibited profound cecal inflammation, bacterial dissemination, and morbidity following S. Typhimurium challenge and greater colonic inflammation throughout the course of C. rodentium infection.”

Rock microbiome

* Halophilic Archaea Cultivated from Surface Sterilized Middle-Late Eocene Rock Salt Are Polyploid – Salla T. Jaakkola – PLOS ONE

“We used real-time PCR to show that our isolates are polyploid, with genome copy numbers of 11–14 genomes per cell in exponential growth phase.”

More Microbiology

Zooming in to see the bigger picture: Microfluidic and nanofabrication tools to study bacteria – Felix J. H. Hol, Cees Dekker – Science

“Here, we review the new scientific insights gained by using a diverse set of nanofabrication and microfluidic techniques to study individual bacteria and multispecies communities.”

Review: Theory and Empiricism in Virulence Evolution – James J. Bull, Adam S. Lauring – PLOS Pathogens

“Our purpose here is to offer a brief introduction to virulence theory, explain some of its strengths and weaknesses, and suggest how theory might be united with empiric data”

Techniques

Comparing the new 16S rRNA V4 and ITS primers to the old primers-RESULTS! – Embriette Hyde – MicroBEnet

“The Knight lab has been working hard testing new primers for 16S rRNA amplicon production and its time to share our progress.”

Microbes in the News

As permafrost soils thaw, soil microbes amplify global climate change – Science Daily

“Now, research by an international team of scientists from the U.S., Sweden and Australia, led by University of Arizona scientists, shows that a single species of microbe, discovered only very recently, is an unexpected key player in climate change.”

* The Scientist: Prof. Esther Angert Studies Extreme Bacteria – Siddesh Ramesh – Cornell Sun

“While the majority of bacteria cannot be seen without a microscope, there exists a group of bacterial species that is visible to the naked eye. Known as Epulopiscium, they are “an extreme on the spectrum of diversity of bacterial life in existence,” according to Prof. Esther Angert, microbiology.”

Bik’s Picks

Flare Alert: Monster Sunspot Turns Toward Earth – Ian O’Neill – Discovery

“Amateur astronomers have been wowed by a vast sunspot that has rotated to face Earth, the largest since this solar cycle began in 2008, and solar observatories (on the ground and orbiting Earth) are closely monitoring the region.“

Watch cell division with new, remarkable resolution: Nobel winner Betzig strikes again – Tech Times

“Just weeks after sharing the Nobel Prize in chemistry for his work involving high-resolution microscopes, a U.S. researcher has again stunned the scientific world with a new technique that peers deeply into living cells.

Ancient Europeans do not drink milk according to new study – BetaWired

“Almost everyone drinks milk today but a new study shows that ancient Europeans were not milk lovers back in the old days. According to a DNA analysis result, ancient Europeans are lactose intolerant.”

[hr]