April 29, 2020

Hello Everyone. Today the Microbiome Digest brings you the latest on Plant-microbial interaction together with a variety of your usual microbiome related research publications. Enjoy.

COVID-19:

Neuraxial anaesthesia and peripheral nerve blocks during the COVID-19 pandemic: a literature review and practice recommendations. – Uppal V. et al. – Anaesthesia.

General microbiome:

The link between autism spectrum disorder and gut microbiota: A scoping review – Nitschke A. et al. – Autism.

General microbiome:

Combined bacterial and fungal targeted amplicon sequencing of respiratory samples: Does the DNA extraction method matter? – Angebault C. et al. – PloS One.

Randomised clinical trial: faecal microbiota transplantation versus autologous placebo administered via colonoscopy in irritable bowel syndrome. – Lahtinen et al. – Aliment Pharmacol Ther.

High L-carnitine ingestion impairs liver function by disordering gut bacteria composition in mice. – Wu Q. et al. – J Agric Food Chem.

Plant, root, and soil microbiome:

Microbiome-Mediated Stress Resistance in Plants – Liu H et al. – Trends in Plant Science.

A plant genetic network for preventing dysbiosis in the phyllosphere – Chen T et al. – Nature.

Harnessing rhizosphere microbiomes for drought-resilient crop production – de Vries et al. – Science

Plant nutrient‐acquisition strategies drive topsoil microbiome structure and function – Bahram et al. – New Phytologist.

Impacts of directed evolution and soil management legacy on the maize rhizobiome – Schmidt JE et al. – Soil Biology and Biochemistry.

Environmental microbiome:

Metal-induced bacterial interactions promote diversity in river-sediment microbiomes. – Cyriaque V. et al. – FEMW Microbiol Ecol.

April 27, 2020

Hi from the UK,

Apart from a couple of animal microbiology papers and a review of the current status of fungal cell wall biotechnology, today’s review focuses on the human microbiome, particularly within the context of medicine and surgery. Recent posts, especially Shirin’s post on the 24th April was so comprehensive that I hope if you are looking for other areas of microbiology you will find plenty there until tomorrow 🙂

Covid-19

A short review on antibody therapy for COVID-19 – New Microbes and New Infections

Structure of Mpro from COVID-19 virus and discovery of its inhibitors – Nature.com

Human gut microbiome

Faecal microbiota transplantation in the treatment of Clostridioides difficile infection – Human Microbiome Journal

Children with autism spectrum disorder: Pilot studies examining the salivary microbiome and implications for gut metabolism and social behaviour – Human Microbiome Journal

Parasitology

Prevalence and genotypic characterization of Giardia duodenalis isolates from asymptomatic school-going children in Lusaka, Zambia – Food and Water Parasitology

Shedding and characterization of gastrointestinal nematodes of growing beef heifers in Central Texas – Veterinary Parasitology X

Phages and viruses

Phylodynamic analysis of HIV-1 subtypes B, C and CRF 02_AG in Senegal – Epidemics

Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next-generation sequencing – Papillomavirus Research

Pacemaker-associated infection caused by ST81/SCCmec IV methicillin-resistant, vancomycin-intermediate Staphylococcus aureus in Japan – New Microbes and New Infections

Prevalence of metallo-β-lactamase–producing Pseudomonas aeruginosa isolated from diabetic foot infections in Iraq – New Microbes and New Infections

Mycobiome

 The fungal cell wall: Biology, biosynthesis and biotechnology – The Cell Surface

Animal microbiome

Dynamics of livestock-associated methicillin-resistant Staphylococcus aureus in pig movement networks: Insight from mathematical modelling and French data – Epidemics

Comparison of gut microbiome from Sulawesi carrion and burying beetles (Coleoptera: Silphidae) through metagenomic analysis of 16S rRNA gene – Bio-conferences.org

As we are saying in the UK: stay home and stay safe.

Karen

 

 

April 19, 2020

Hello from the Sonoran Desert! It is a lovely morning here in Tucson, Az. I am excited to send my first contribution to Microbiome Digest! There is a lot of diversity in today’s digest, but if microbes thriving in all kinds of strange, extreme conditions are your area of interest, you’ll be interested in the book compiling the latest studies on extremophiles. Each chapter focuses on a particular environment, ranging from deserts to deep-sea hydrothermal vents, and explores the mechanisms allowing microbes to survive these incredible conditions.

If like me, you are a computational biology enthusiast and a machine learning addict, you should enjoy reading the last entry, a short perspective paper examining how machine learning algorithms can help predict antibiotic resistance!

Have a great Sunday!


General microbiology

Preprint: Interspecies interactions in bacterial colonies are determined by physiological traits and the environment – Booth – BioRxiv


Human gut and mouth microbiome

Bacterial colonization reprograms the neonatal gut metabolome – Bittinger – Nature microbiology

Salivary Factors that Maintain the Normal Oral Commensal Microflora – Carpenter – Journal of dental research

Preprint: Metagenomic Next-Generation Sequencing of Rectal Swabs for the Surveillance of Antimicrobial Resistant Organisms on the Illumina Miseq and Oxford MinION Platforms – Yee – bioRxiv


Animal microbiome

Preprint: Mortality of native and invasive ladybirds co-infected by ectoparasitic and entomopathogenic fungi – Haelewaters – bioRxiv

Built environment

Preprint: The Microbes Found in the Honey of New York City Beehives – Weiss – BioRxiv

Correlation between autochthonous microbial communities and key odorants during the fermentation of red pepper (Capsicum annuum L.) – Xu – Food Microbiology

Water and extremophile microbiome

Book: Microbial Versatility in Varied Environments: Microbes in Sensitive Environments – collective – Springer Nature

The seasonal variation of microbial communities in drinking water sources in Shanghai – Guo – Journal of Cleaner Production

Plant, root and soil microbiome

LDPE microplastics significantly alter the temporal turnover of soil microbial communities –  Wang – Science of the total environment

High abundance of Ralstonia solanacearum changed tomato rhizosphere microbiome and metabolome – Wen – BMC Plant Biology


Phages and viruses

Preprint : First evidence of virus-like particles in the bacterial symbionts of Bryozoa – Vishnyakov – BioRxiv

Microbial community development on model particles in the deep sulfidic waters of the Black Sea – Suominen – Environmental microbiology

The stepwise assembly of the neonatal virome is modulated by breastfeeding – Liang – Nature

Bioinformatics

Preprint: Bayesian classification, anomaly detection, and survival analysis using network inputs with application to the microbiome – Josephs – arXiv

Techniques

Preprint: Expanding the diversity of bacterioplankton isolates and modeling isolation efficacy with large scale dilution-to-extinction cultivation – Henson – bioRxiv

Catalyzing rapid discovery of gold-precipitating bacterial lineages with university students – Riley – PeerJ


COVID-19 and career

Science-ing from home – Powell – Nature

My non-microbiology pick

Perspective: Lean, mean, learning machines – Wheeler – Nature review microbiology

COVID-19 and Staying Sane

We are living in an insane time. Within the past month, a lot of the world has been put on lockdown, toilet paper is a rare commodity for some reason, and a new virus is sweeping across the world. For many researchers such as myself, this pandemic has shut down a huge portion of our lives since labs are no longer open. Terrifyingly enough, we now have to get used to doing all our work cooped up in our homes. 

When working at home, it’s extremely easy to beat yourself up for not being as driven or productive as you would like, but here is the most important thing for you to remember: NO ONE IS EXPECTING YOU TO BE AS PRODUCTIVE AS YOU WERE BEFORE. This pandemic is unprecedented, and it is causing everyone’s lives to go haywire. Adjusting to such big changes is hard, and it’s unreasonable for anyone to expect you to maintain the same level of productivity during these crazy times. 

For many researchers, lab work is a huge portion of time that is suddenly gone. At least for me, filling the hole that the lack of research has left has been challenging. However, there are still plenty of opportunities to keep working and building research skills while social distancing. Here are a couple of things: 

  1. Learn a new programming language. Codeacademy is giving away Pro memberships to the first 10,000 people to sign up with a .edu email address because of the COVID-19 pandemic. Although Codeacademy is meant for absolute beginners, it does provide a good jumping-off point into further work with programming. Aside from Codeacademy, Datacamp has courses in data analysis languages as well, covering Python, R, Command-Line, and more. 
  2. Brush up on your bioinformatics. There are some really great resources available online tutorials and example datasets to practice on. Bioconductor, for instance, has a huge list of online resources and example data sets to practice using R as a biologist. There are also many tutorials online for using the command-line in bioinformatics, one of which can be found here
  3. Use your social media as a scientist. Now more than ever, Twitter is the perfect place to read about the newest advances in science and jump in on the discussion as well. Even on topics unrelated to COVID-19, scientific discussion is thriving now more than ever. 
  4. Work on your writing skills. While this is probably obvious to any researcher, I still feel like I should mention this. Take this time to write a new review, finally write that Materials and Methods section, or just finish up anything that might be waiting on your to-do list. 
  5. Find out more about something random that interests you by reading a bunch of literature. Whether this is just catching up on a huge backlog of papers you need to get around to reading, or diving headfirst into a completely new field, 

But what can you do outside of work to stay sane? One great thing you can do to fill this new time is to find a new hobby! Baking bread is weirdly popular now, with my coworkers sharing recipes and pictures of the masterpieces they made (if you’re looking for a neat recipe, check out this one). If you’re also looking to contribute to stopping the spread of COVID-19, you could do something simple like sewing face masks as well.

One of the most important things you have to do during this time is to take mental health days. This could be as simple as just taking one day a week to not think about work and focus on relaxing. These breaks can go a long way in reminding you that work doesn’t control your life, even though it might seem like that now more than ever. 

This is a difficult time for everyone, and learning how to adapt to a life without physical work is extremely challenging. By taking small steps, we can all learn to adapt and come out of this pandemic as stronger researchers.

April 15, 2020

Hi folks! Another new volunteer poster here up from the ranks of the recently recruited. Today’s post is pre-print and plant/soil heavy. If up until now you’ve focused your reading on animal microbiomes, now’s a great time to check in on our photosynthesizing friends. Lots of great work today on the affect of management strategies on plant-associated and soil microbes. If the transition into the world of the leafy green is too jarring, perhaps try easing yourself in with Marchese et al.’s work on the mycobiome of sea cucumbers – a critter which is plant in common name only.

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Pregnancy and early life

Review: Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health – Lyons et al. – Nutrients

Human respiratory microbiome

Comment: Microbiota and chronic lung disease in preterm infants. Where is the truth? – Nandakumar and Aly – Journal of Perinatology

Human skin microbiome

The cutaneous microbiome in hospitalized patients with pressure ulcers – de Wert Luuk et al. – Scientific Reports

Human vaginal microbiome

Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota – Manhanzva et al. – Scientific Reports

A meta-analysis of mannose-binding lectin gene polymorphisms with the risk of recurrent vulvovaginal infections – Kalia et al. – Scientific Reports

Human gut microbiome

Review: Gut Microbial Metabolites of Aromatic Amino Acids as Signals in Host–Microbe Interplay – Liu et al. – Trends in Endocrinology & Metabolism

Preprint: Computational Modeling of the Gut Microbiota Predicts Metabolic Mechanisms of Recurrent Clostridioides difficile Infection – Henson – bioRxiv

Preprint: Comparative genomics reveals different population structures associated with host and geographic origin in antimicrobial-resistant Salmonella enterica – Liao et al. – bioRxiv

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Animal experiments

Preprint: Validating the use of bovine buccal sampling as a proxy for the rumen microbiota using a time course and random forest classification approach – Young et al. – bioRxiv

Animal microbiome

Gut microbial diversity across a contact zone for California voles: implications for lineage divergence of hosts and mito‐nuclear mismatch in the assembly of the mammalian gut microbiome – Lin et al. – Molecular Ecology

Diversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli: disclosing the strains potential for biomedical applications – Marchese et al. – Journal of Applied Microbiology

Perspective: Microbiome maturation during a unique developmental window – Videvall – Molecular Ecology

Preprint: Microbial interactions in the mosquito gut determine Serratia colonization and blood feeding propensity – Kozlova et al. – bioRxiv

Plant, root, and soil microbiome

Reduced microbial diversity induces larger volatile organic compound emissions from soils – Abis et al. – Scientific Reports

Increased temperatures alter viable microbial biomass, ammonia oxidizing bacteria and extracellular enzymatic activities in Antarctic soils – Barnard et al. – FEMS Microbiology Ecology

One ring to rule them all: an ecosystem engineer fungus fosters plant and microbial diversity in a Mediterranean grassland – Zotti et al. – New Phytologist

Changes in soil fungal communities following anthropogenic disturbance are linked to decreased lodgepole pine seedling performance – Beck et al. – Journal of Applied Ecology

Long-term effects of stump removal and tree species composition on the diversity and structure of soil fungal communities – Modi et al. – FEMS Microbiology Ecology

Effect of treated sewage characteristics on duckweed biomass production and microbial communities – Iwano et al. – Water Science and Technology

Review: Functional roles of microbial symbionts in plant cold tolerance – Acuña‐Rodríguez et al. – Ecology Letters

Preprint: Dryland cropping system, weed communities, and disease status modulate the effect of climate conditions on wheat soil bacterial communities – Ishaq et al. – bioRxiv

Preprint: Cropping systems that improve richness convey greater resistance and resilience to soil fungal, relative to prokaryote, communities – Finn et al. – bioRxiv

Water and extremophile microbiome

Diverse groups of fungi are associated with plastics in the surface waters of the Western South Atlantic and the Antarctic Peninsula – Lacerda et al. – Molecular Ecology

Nitrification kinetics and microbial community dynamics of attached biofilm in wastewater treatment – Skoyles et al. – Water Science and Technology

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Techniques

Data normalization strategies in metabolomics: Current challenges, approaches, and tools – Misra – European Journal of Mass Spectrometry

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Microbes on the market

Blog: COVID-19: Impact of Coronavirus Pandemic on Human Microbiome Market 2020. Top Leaders are Dohme Corp, Seres Therapeutics, Synthetic Biologics Inc, Synlogic – Publicist 360

 

 

April 14, 2020

Hello,

Hope this finds you all well. This is my first post, and hopefully not the last. Here are some interesting articles for you to enjoy today. You may notice that I will end up posting a lot about stress, sleep, and mental illness. I’m pretty diverse and also have a soft spot for nature.

Happy reading.

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  • Pregnancy and early life

Review: Maternal Vertical Transmission Affecting Early-life Microbiota Development S. Wang, et al.

  • Human skin microbiome

Bacteriophage of the Skin Microbiome in Patients with Psoriasis and Healthy Family Controls H. Wang, et al.

  • Human vaginal microbiome

Clinical Research: A 28 Day Clinical Assessment of a Lactic Acid-containing Antimicrobial Intimate Gel Wash Formulation on Skin Tolerance and Impact on the Vulvar Microbiome E. Bruning, et al.

  • Human gut microbiome

Altered Fecal Microbiome Years after Traumatic Brain Injury R. J. Urban, et al.

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  • Animal experiments (mouse/rat experiments, chickens/pigs on different diets)

A Uniquely Altered Oral Microbiome Composition Was Observed in Pregnant Rats With Porphyromonas gingivalis Induced Periodontal Disease M.S. Walkenhorst, et al.

  • Animal microbiome (for animals in the wild)

Preprint: Gut microbiome modulates behaviour and life history in two wild rodents J. W. Jameson, et al.

  • Plant, root, and soil microbiome

Review: Recent advances in the role of plant metabolites in shaping the root microbiome R.P. Jacoby, et al.

April 13, 2020

Hi everyone!

In today’s digest there is a range of exciting papers. The first paper looks into using a COVID-19 RBD vaccine on rodents. You will also find a great study on utilising Myxobacterium to fight cucumber wilt – even as an avid cucumber hater I appreciated this one! A third highlight is an exciting clinical study of heat-inactivated Bifidobacterium in reducing patient IBD symptoms.

COVID-19

Preprint: The SARS-CoV-2 receptor-binding domain elicits a potent neutralizing response without antibody-dependent enhancement – Quinlan et al.

Preprint: Comparison of SARS-CoV-2 infections among 3 species of non-human primates – Lu et al.

Preprint: Three adjacent nucleotide changes spanning two residues in SARS-CoV-2 nucleoprotein: possible homologous recombination from the transcription-regulating sequence – Leary et al.

Human gut microbiome

Preprint: Short-chain fatty acid production by gut microbiota from children with obesity is linked to bacterial community composition and prebiotic choice – Holmes et al.

Short Review: In sickness and health: Effects of gut microbial metabolites on human physiology – Glowacki & Martens. PLOS Pathogens.

Animal experiments

Evaluation of microbiome-host relationships in the zebrafish
gastrointestinal system reveals adaptive immunity is a target of bis(2-ethylhexyl) phthalate (DEHP) exposure
– Adamovsky et al. Environmental Science & Technology.

Abnormal gut microbiota composition is associated with experimental autoimmune prostatitis‐induced depressive‐like behaviors in mice – Du et al. The Prostate.

Plant, root and soil microbiome

Impact of phages on soil bacterial communities and nitrogen availability under different assembly scenarios – P. Braga et al. Microbiome.

Predatory myxobacterium controls cucumber fusarium wilt by regulating soil microbial community – Ye et al. Microbiome.

Food Microbiology

Green tea polyphenols boost gut-microbiota-dependent mitochondrial TCA and urea cycles in Sprague–Dawley rats – Zhou et al. The Journal of Nutritional Biochemistry.

Probiotics

Heat-inactivated Bifidobacterium bifidum MIMBb75 (SYN-HI-001) in the treatment of irritable bowel syndrome: amulticentre, randomised, double-blind, placebo-controlled clinical trial – Andresen et al. The Lancet Gastroentrology & Hepatology.

April 12, 2020

¡Buenos dias!

Greetings from Spain as I write my first blog post, I am very excited to have been given this opportunity and I hope I can send a little bit of this enthusiasm to all of you in this sunday morning. In today’s menu we have a really interesting review of Microbiome-Neuronal Signaling along with another of human breast milk with a curius subsection of It’s miRNA composition. All this with many other publications from biorreactors to soil microbiome . I hope you enjoy and forgive my little mistakes.

Hope to see you soon. Adios!

COVID-19

Preprint: A virus that has gone viral: Amino acid mutation in S protein of Indian isolate of Coronavirus COVID-19 might impact receptor binding and thus infectivity Priyanka Saha in bioRxiv

Preprint: Potential host range of multiple SARS-like coronaviruses and an improved ACE2-Fc variant that is potent against both SARS-CoV-2 and SARS-CoV-1 Yujun Li et al. in bioRxiv

Preprint: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing Devon Chandler-Brown in bioRxiv

Preprint: Nonmedical Masks in Public for Respiratory Pandemics: Droplet Retention by two-layer Textile Barrier Fully Protects Germ-free Mice from Bacteria in Droplets Alexander Rodriguez-Palacios et al. in bioRxiv

General Microbiome

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213 Alice Senizza et al. in Scientific Reports

Human Gut Microbiome

Preprint: Not just ‘immunity’: how the microbiota can reshape our approach to cancer immunotherapy Federico Boem et al. in Immunotherapy

Review: Toward Understanding Microbiome-Neuronal Signaling K. G. Jameson et al. in Molecullar Cell.

Gut microbiota in children with juvenile idiopathic arthritis: characteristics, biomarker identification, and usefulness in clinical prediction Xubo Qian et al. in BMC Genomics

Fecal-associated microbiome differences between traditional Chinese medicine qi-deficiency and balanced constitutions Jianhua Zhen et al. in Journal of Traditional Chinese Medical Sciences

Gut Microbiota Modulate CD8 T Cell Responses to Influence Colitis-Associated Tumorigenesis Amy I.Yu et al. in Cell Reports

Human Vaginal Microbiome

Review: Recommendations on Screening for Asymptomatic Bacterial Vaginosis in Pregnancy Amanda L. et al. in JAMA

Human nearly-sterile sites

Preprint: Changes to the resistome of Pseudomonas aeruginosa clone ST308 associated with corneal infection over time Mahjabeen Khan et al. in bioRxiv

Pregnancy and early life

Review: Components of human breast milk: from macronutrient to microbiome and microRNA. Su Yeong Kim et al. in Clinical and experimental pediatrics

Animal experiments

The impact of short-term acute heat stress on the rumen microbiome of Hanwoo steers Youl Chang Baek et al. in Journal of Animal Science and Technology (JAST)

Effects of Dietary Microencapsulated Tannic Acid (extracted from Chinese gallnut) Supplementation on the Growth Performance, Intestinal Morphology, and Intestinal Microbiota in Weaning Piglets Meiwei Wang et al. in Journal of Animal Science

Association between oropharyngeal microbiome and weight gain in piglets during pre and post weaning life Andrew Wange Bugenyi et al. in Journal of Animal Science and Technology (JAST)

An early fecal microbiota transfer improves the intestinal conditions on microflora and immunoglobulin and antimicrobial peptides in piglets Teng Teng et al. in Journal of Agricultural and Food Chemistry

Microbial communities and food safety aspects of crickets (Acheta domesticus) reared under controlled conditions X. Fernandez-Cassi et al. in Journal of Insects as Food and Feed

Difference of gut microbiota composition based on the body condition scores in dogs Ju Lan Chun et al. in Journal of Animal Science and Technology (JAST)

Preprint: A Systematic Analysis of Mosquito-Microbiome Biosynthetic Gene Clusters Reveals Antimalarial Siderophores that Reduce Mosquito Reproduction Capacity Jack G Ganley et el. in bioRxiv

Plant, root, and soil microbiome

Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla Yakshi Ortiz et al. in PLOS ONE

Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil Ana Flavia Tonelli Fernandes et al. in Microbes and Environments

Prepint: An intensive multilocation temporal dataset of fungal communities in the root and rhizosphere of Brassica napus NavidBazghaleh in Data et al. in Brief

Comammox bacterial abundance, activity, and contribution in agricultural rhizosphere soils XiaominWang et al. in Science of The Total Environment

Diversity and potential biogeochemical impacts of viruses in bulk and rhizosphere soils Li Bi et al. in Environ Microbiology

Rhizosphere microbiome assemble and its impact on plant growth Qian Qu et al. in Journal of Agricultural and Food Chemistry

Water and extremophile microbiome

Patterns in extracellular enzyme activity and microbial diversity in deep-sea Mediterranean sediments NagissaMahmoudi et al. in Deep Sea Research

Preprint: Towards a probiotic approach for building plumbing – Nutrient-based selection during biofilm formation on flexible polymeric materials Lisa Neu in bioRxiv

Built environment

The biogeography and assembly of microbial communities in wastewater treatment plants in China Bing Zhang et al. in Environmental Science & Technology

Aerobic biodegradation of p-nitrophenol in a nitrifying sludge bioreactor: System performance, sludge property and microbial community shift MingrunLi et al. in Journal of Environmental Management

Microbes on the market

Blog: Microbiome Therapeutics Market 2020 Latest Trends, Technology Studies, Substantial Growth, Key Developments and Future Analysis till 2025 Anita in Science In Me

Blog: Microbiome Sequencing Services Market: Opportunities and Forecast Assessment, 2019-2025 supriya in Curius Desk

Techniques

preprint: Combining light sheet microscopy and expansion microscopy for fast 3D imaging of virus-infected cells with super-resolution Luca Mascheroni et al. in bioRxiv

April 11, 2020

Happy Saturday morning, y’all! I’m excited for my first contribution to the digest, and hope you enjoy my picks over a luxurious weekend meal. Today’s digest starts with some preprints about COVID including research on a drug that may inhibit viral replication. Other research highlights include an examination of the relationship between the vaginal microbiome and pre-term birth, a preprint on the oral microbiome in space, and a potential new technique for low cost, high-throughput metagenome profiling. Scroll all the way to the end for information about a fun project by a University of Wyoming student called The Microbestiary.

COVID-19
Preprint: Genomic determinants of pathogenicity in SARS-CoV-2 and other human coronaviruses. Auslander, N. et al.

Preprint: Nelfinavir inhibits replication of severe acute respiratory syndrome coronavirus 2 in vitro. Yamamoto, N. et al.

Preprint: Understanding the B and T cells epitopes of spike protein of severe respiratory syndrome coronavirus-2: A computational way to predict the immunogens. Vashi, Y. et al.

General Microbiome
Preprint: From iron to antibiotics: Identification of conserved bacterial-fungal interactions across diverse partners. Pierce, EC. et al.

Human Gut Microbiome
Review: Unraveling Host-Gut Microbiota Dialogue and Its Impact on Cholesterol Levels. Villette R. et al. Frontiers in Pharmacology.

Metagenomics analysis reveals features unique to Indian distal gut microbiota. Kaur, K. et al. PLOS ONE.

Gut Microbiota Modulate CD8 T Cell Responses to Influence Colitis-Associated Tumorigenesis. Yu, A.I. et al. Cell Reports.

Human Oral Microbiome
Preprint: Characterization of the oral microbiome of medically controlled type-2 diabetes patients. Santos, A. et al.

Human Vaginal Microbiome
Cervicovaginal microbiome and natural history of HPV in a longitudinal study. Usyk, M. et al. PLOS Pathogens.

Meta-Analysis of Vaginal Microbiome Data Provides New Insights Into Preterm Birth. Kosti, I. et al. Frontiers in Microbiology.

Water and Extremophile Microbiome
Microbiota characterization of Exaiptasia diaphana from the Great Barrier Reef. Hartman, L.M. et al. Animal Microbiome.

Microbial Diversity and Metabolic Potential in the Stratified Sansha Yongle Blue Hole in the South China Sea. He, P. et al. Scientific Reports.

Metabolic versatility of freshwater sedimentary archaea feeding on different organic carbon sources. Compte-Port, S. et al. PLOS ONE.

Animal Experiments and Animal Microbiome
The skin microbiome facilitates adaptive tetrodotoxin production in poisonous newts. Vaelli, P.M. et al. eLife.

Metatranscriptomic virome assessment of Rhipicephalus microplus from Colombia. Gomez, G.F. et al. Ticks and Tick-borne Diseases.

Preprint: Metabolic differences between symbiont subpopulations in the deep-sea tubeworm Riftia pachyptila. Hinzke, T. et al.

Preprint: Similar gut bacterial microbiota in two fruit-feeding moth pests collected from different host species and locations. Gong, Q et al.

Plant, Root, and Soil Microbiome
The preceding root system drives the composition and function of the rhizosphere microbiome. Zhou, Y. et al. Genome Biology.

Microbial Co-Occurrence in Floral Nectar Affects Metabolites and Attractiveness to a Generalist Pollinator. Rering, C.C. et al. Journal of Chemical Ecology.

Impact of phages on soil bacterial communities and nitrogen availability under different assembly scenarios. Braga, L.P.P. et al. Microbiome.

A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community. Ye, X. et al. Microbiome.

A plant genetic network for preventing dysbiosis in the phyllosphere. Chen, T. et al. Nature.

Fungal communities differentially respond to warming and drought in tropical grassland soil. de Oliveira, T.B. et al. Molecular Ecology.

Built Environment
An in-depth survey of the microbial landscape of the walls of a neonatal operating room. Derilus, D. et al. PLOS ONE.

Microbes in Space
Preprint: Defining the resilience of the human salivary microbiota by a 520 days longitudinal study in confined environment: the Mars500 mission. Bacci, G. et al.

Phages and Viruses
Phage cocktail containing Podoviridae and Myoviridae bacteriophages inhibits the growth of Pectobacterium spp. under in vitro and in vivo conditions. Zaczek-Moczydłowska, M.A. et al. PLOS ONE.

Dynamic genome evolution and complex virocell metabolism of globally-distributed giant viruses. Moniruzzaman M., et al. Nature Communications.

Preprint: Infection with novel Bacteroides phage BV01 alters host transcriptome and bile acid metabolism in a common human gut microbe. Campbell, D.E. et al.

Bioinformatics
Preprint: Integrative and Conjugative Elements (ICE) and Associated Cargo Genes within and across Hundreds of Bacterial Genera. Kaufman, J. et al.

Techniques
A restriction enzyme reduced representation sequencing approach for low-cost, high-throughput metagenome profiling. Hess, M.K. et al. PLOS ONE.

Industrial Microbiome
Open microbiome dominated by Clostridium and Eubacterium converts methanol into i-butyrate and n-butyrate. Huang, S. et al. Applied Microbiology and Biotechnology.

Fun with Microbes
University of Wyoming masters student Erin Bentley was selected for an Arctic Circle Expedition in 2021 where she will characterize microbes and share her discoveries on The Microbestiary.
“The Microbestiary strives to show the public, in welcoming and memorable ways, that the microbial world is populated by strange and beautiful characters,” says Bentley in an article published on the UW website.

April 08, 2020

Good morning. This is my first contribution to the Microbiome Digest. It is an interesting opportunity to survey some of the current literature and areas of research, and I hope that the literature summarized below will be of interest to readers. Today’s digest also includes articles on covid-19. There are two publications on metabolomic and/or proteomic profiling of covid-19 patients, and one which analyzes genetic variants of the ACE-2 protein across a database taken from the Italian population. A topic of personal interest to me is how and whether the level of glycosylation and sialylation on the ACE-2 protein and on the coronavirus spike protein may influence binding and covid-19 infectivity. However, this is not covered in the publication.

The two papers in the pregnancy and early life section discuss the development of the infant microbiome, with regard to dysbiosis in preterm infants and with regard to the contribution of maternal milk-derived microbes.

The extremophile paper by Roy et al. looks at the microbial population in boron-rich extremophile environments. Alkyl borates are known to form boronate complexes with saccharides and it would be interesting to profile the enzymatic functions of these strains for potential borate-metabolizing capability.

The techniques paper by Fergusson et al. describes the building of a mass spectral database by which Burkholderia and related bacterial strains can be identified from their MALDI-TOF metabolite profile, using the Bruker Biotyper platform. Both nucleic acid sequencing and metaproteomic tools were invoked by An et al., in their Ecotoxicology paper investigating the mechanism of microbial metabolism of toxic dye molecules in the environment.

Several papers discuss correlations between microbial populations and disease states. For example, Tengeler’s paper describes the use of MRI to to study the effects on the brains of germ-free mouse brains, following transplantation of gut bacteria from humans with and without ADHD, while Zheng et al. describe microbiome signatures which correlate with lung cancer.

Under the Market Analysis section, a $38M deal between Second Genome and Gilead has been announced.

Covid-19
Preprint:
The relationship of COVID-19 severity with cardiovascular disease and its traditional risk factors: A systematic review and meta-analysis
Matsushita, K. et al.

Preprint:
Repurposing Therapeutics for COVID-19: Rapid Prediction of Commercially available drugs through Machine Learning and Docking
Mahapatra, S. et al.

Preprint:
Proteomic and Metabolomic Characterization of COVID-19 Patient Sera
Bo Shen et al.

Preprint:
Plasma Metabolomic and Lipidomic Alterations Associated with COVID-19
Wu, D. et al.

Preprint:
Estimating false-negative detection rate of SARS-CoV-2 by RT-PCR
Wikramaratna, P. et al.

Preprint:
ACE2 variants underlie interindividual variability and susceptibility to COVID-19 in Italian population
Renieri, A. et al.

General Microbiome

MP10-18 FUNCTIONAL ANALYSIS OF THE URINARY TRACT MICROBIOME OF CALCIUM OXALATE STONE FORMERS
N Kachroo et al,
The Journal of Urology, 2020

Microbial Engineering Community (mce): Selecting Key Players in Microbiomes
CEG Camacho et al.
Chemical Engineering Transactions, 2020

Pregnancy and early life

Contributors to Dysbiosis in Very-Low-Birth-Weight Infants
MW Groer et al.

Journal of Obstetric, Gynecologic, & Neonatal Nursing, 2020

A cross-sectional pilot study of birth mode and vaginal microbiota in reproductive-age women
CA Stennett et al.
PLOS one, 2020

Milk Microbiome and Neonatal Colonization: Overview.
S Rautava
Nestle Nutrition Institute workshop series, 2020

Oral Microbiome

Single-Cell Genomics and the Oral Microbiome
M Podar et al.
Journal of Dental Research, 2020

Dysbiosis of saliva microbiome in patients with oral lichen planus
Fei Yan Yu et al. BMC Microbiology, 2020

Gut Microbiome

Specific gut microbiome signature predicts the early-stage lung cancer
Yajuan Zheng et al.
Gut Microbes, 2020

Complex influences of gut microbiome metabolism on various drug responses
Sihyun Chae et al.
Translational and Clinical Pharmacology, 2020

Gut Microbiota, Its Role in Induction of Alzheimer’s Disease Pathology, and Possible Therapeutic Interventions: Special Focus on Anthocyanins
MS Khan et al.
Cells, 2020

Faecal microbiota transplantation: indications, evidence and safety
WT Soo et al.
Australian Prescriber, 2020

PhD Thesis:
The effect of faecal microbiota transplantation in irritable bowel syndrome. A double blind, randomized placebo controlled single centre study
Peter Johnsen

Animal Experiments
Gut microbiota from persons with attention-deficit/hyperactivity disorder affects the brain in mice
Anouk C. Tengeler et al.
Microbiome 2020

Aberrant gut microbiota alters host metabolome and impacts renal failure in humans and rodents
Xifan Wang et al.
Gut, 2020

Animal Microbiome
The microbiota of dairy milk: a review
Eugenio Parente
International Dairy Journal, 2020

Plant, root and soil Microbiome
Comparative analysis of the total and active bacterial communities in the surface sediment of Lake Taihu
T Liu et al.
FEMS Microbiology Ecology, 2020

Recovery of fen peatland microbiomes and predicted functional profiles after rewetting
WJ Emsens et al.
The ISME Journal, 2020

Microbial assemblages associated with the rhizosphere and endosphere of an herbage, Leymus chinensis.
J Chen et al.
Microbial biotechnology, 2020

Insights into the earthworm gut multi-kingdom microbial communities
R Sapkota et al.
Science of the Total Environment, 2020

Prevalent root-derived phenolics drive shifts in microbial community composition and prime decomposition in forest soil
MJ Zwetsloot et al.
Soil Biology and Biochemistry, 2020

Extremophile Microbiomes
Abiotic factors influence patterns of bacterial diversity and community composition in the Dry Valleys of Antarctica
EM Bottos et al.
FEMS Microbiology Ecology, 2020

Structure and function of the Arctic and Antarctic marine microbiota as revealed by metagenomics
Weipeng Zhang et al.
Microbiome 2020

Integrated metagenomic and metaproteomic analyses reveal potential degradation mechanism of azo dye-Direct Black G by thermophilic microflora
X An et al.
Ecotoxicology and Environmental Safety, 2020

Microbiome and ecology of a hot spring-microbialite system on the Trans-Himalayan Plateau
C Roy et al.
Scientific Reports, 2020

Microbes in Built Environments

An in-depth survey of the microbial landscape of the walls of a neonatal operating room
D Derilus et al.
PLOS ONE, 2020

Phages and Viruses

Preprint:
Infection with novel Bacteroides phage BV01 alters host transcriptome and bile acid metabolism in a common human gut microbe
DE Campbell et al.

Bioinformatics and Techniques

Development of a Custom MALDI-TOF Mass Spectrometric Database for Identification of Environmental Burkholderia and Related Genera.
CH Fergusson et al.
Environmental Microbiology, 2020

MetaEuk—sensitive, high-throughput gene discovery, and annotation for large-scale eukaryotic metagenomics
Eli Levy Karin et al.
Microbiome 2020

Inference-based accuracy of metagenome prediction tools varies across sample types and functional categories
Shan Sun et al.
Microbiome 2020

Preprint:
DNA Thermo-Protection Facilitates Whole Genome Sequencing of Mycobacteria Direct from Clinical Samples by the Nanopore Platform
Sophie George et al.

Preprint:
Seeker: Alignment-free identification of bacteriophage genomes by deep learning
Noam Auslander et al.

Multiview learning for understanding functional multiomics
ND Nguyen et al.

PLOS Computational Biology, 2020

Microbes on the Market

News Article:
Gilead agrees microbiome deal with Second Genome
Ben Hargreaves, Biopharma Reporter

Market Report:
Human Microbiome Market Future Outlook 2020-2026. Major Players are Dohme Corp, Seres …
Science In Me

Market Report:
Europe Human Microbiome Market to Offer Increased Growth Prospects for Manufacturers up to 2025
Sameer Joshi, Curious Desk (blog)