Short digest for today, but I’d like to draw attention to two studies on creative techniques to reduce contamination by host DNA sequences: one using PCR blocking primers to improve 16S rDNA sequencing, and the other on using CRISPR-Cas systems to assist with 18S rDNA sequencing. Happy reading!
Human gut microbiome
Review: Gut Microbial Metabolites and Blood Pressure Regulation: Focus on SCFAs and TMAO – Poll et al. – Physiology
Review: Trial Watch: the gut microbiota as a tool to boost the clinical efficacy of anticancer immunotherapy – Daillerè et al. – OncoImmunology
Human oral microbiome
Well‐maintained patients with a history of periodontitis still harbor a more disbiotic microbiome than health – Lu et al. – Periodontology
Plant, root, and soil microbiome
Contrasting soil microbial abundance and diversity on and between pasture drill rows in the third growing season after sowing – Hayes et al. – Renewable Agriculture and Food Systems
A novel PCR clamping assay reducing plant host DNA amplification significantly improves prokaryotic endo-microbiome community characterization – Lefèvre et al. – FEMS Microbiology Ecology
Water and extremophile microbiome
Diverse viruses have restricted biogeography in deep-sea hydrothermal vent fluids – Thomas et al. – bioRxiv
Mercury methylation by metabolically versatile and cosmopolitan marine bacteria – Lin et al. – bioRxiv
Phages and viruses
A tissue level atlas of the healthy human virome – Kumata et al. – BMC Biology
Review: Deep Roots and Splendid Boughs of the Global Plant Virome – Dolja et al. – Annual Review of Phytopathology
Bioinformatics
An empirical Bayes approach to normalization and differential abundance testing for microbiome data – Liu et al. – BMC Bioinformatics
Techniques
The use of CRISPR-Cas Selective Amplicon Sequencing (CCSAS) to reveal the eukaryotic microbiome of metazoans – Zhong et al. – bioRxiv